No bakteriofāga GA atvasināto mozaikālo vīrusiem līdzīgo daļiņu sintēze raugos Saccharomyces cerevisiae

6533b832fe1ef96bd1299d3a

RESEARCH PRODUCT

No bakteriofāga GA atvasināto mozaikālo vīrusiem līdzīgo daļiņu sintēze raugos Saccharomyces cerevisiae

Dagnija ĀRgule

subject

description

Šajā bakalaura darbā ir parādīta no bakteriofāga GA atvasinātu vīrusiem līdzīgo daļiņu (VLD) sintēze raugos Saccharomyces cerevisiae, izmantojot komerciālo plazmīdu pESC-URA. Proteīnu ekspresijas līmenis tika paaugstināts izmantojot raugiem optimālākus aminoskābes kodējošus kodonus. Pēc VLD ieguvuma tika salīdzināta abu plazmīdā esošo promoteru aktivitāte. Izmantojot divus promoterus, tika veikta divu veidu mozaikālo VLD sintēze, kas sastāv no dabiskā GA apvalka proteīna un N-termināli ar HIV-1 Tat sekvenci vai C-termināli ar Rietumnīlas vīrus proteīna E DIII domēna sekvenci pagarināta GA apvalka proteīna. Tika veikta interleikīna-2 mRNS iepakošana no bakteriofāga GA atvasinātās VLD in vivo. Šis darbs izstrādāts Latvijas Biomedicīnas pētījumu un studiju centrā laika posmā no 2010. gada janvāra līdz 2011. gada maijam. Atslēgvārdi: VLD, iepakošana in vivo, HIV-1 Tat, Rietumnīlas vīruss

year	journal	country	edition	language
2011-01-01

https://dspace.lu.lv/dspace/handle/7/14873

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