Latvijā sastopamo Mycobacterium tuberculosis izolātu visa genoma ģenētiskās dažādības analīze

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RESEARCH PRODUCT

Latvijā sastopamo Mycobacterium tuberculosis izolātu visa genoma ģenētiskās dažādības analīze

Ivars Silamiķelis

subject

description

Tuberkuloze ir plaušu infekcijas slimība, kuru izraisa baktērija Mycobacterium tuberculosis. Starp dažādiem Mycobacterium tuberculosis celmiem novērota atšķirīga ietekme uz infekcijas norises gaitu. Pilna genoma sekvencēšana ir īpaši piemērota metode šīs baktērijas ģenētiskās piederības analīzei. Pētījumā tika analizēta ģenētiskā piederība septiņiem Latvijā sastopamiem Mycobacterium tuberculosis izolātiem, pamatojoties uz viena nukleotīda variācijām un insercijas sekvences IS6110 integrācijas pozīcijām genomā. Dati tika iegūti, izmantojot Ion Torrent nākamās paaudzes sekvencēšanas tehnoloģijas. Datu analīze tika veikta ar datorprogrammu GATK, Samtools, snpEff, VarScan, BRAT, Trimmomatic, FastTree, Mega 5.0 un programmēšanas valodu Perl, Python un C palīdzību. Visiem septiņiem izolātiem tika noteikta ģenētiskā piederība pie LAM saimes LAM-RUS ģenētiskās apakšgrupas. Iegūtos rezultātus iespējams pielietot jaunu Mycobacterium tuberculosis celmu genotipēšanas algoritmu izstrādē. Atslēgvārdi: Nākamās paaudzes sekvencēšana, Mycobacterium tuberculosis, filoģenētiskā analīze

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/26913

PEPhysical Sciences and Engineering
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SHSocial Sciences and Humanities
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