Bakteriofāga Enc34 replikācijas moduļa proteīna ORF6 struktūras noteikšana un analīze

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Bakteriofāga Enc34 replikācijas moduļa proteīna ORF6 struktūras noteikšana un analīze

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Pašlaik genoma datubāzēs ir deponētas vairāk nekā 1200 bakteriofāgu (fāgu) genomu sekvences. Vairums fāgu proteīnu tiek apzīmēti kā konservatīvi vai hipotētiski, un to struktūra un funkcijas pašlaik nav zināmas. Darba mērķis bija noteikt fāga Enc34 proteīna ORF6 terciāro struktūru un prognozēt tā funkciju. Pētījumā sekmīgi tika veikta natīva un ar selenometionīnu iezīmēta ORF6 proteīna ekspresija, attīrīšana un kristalizācija. Ar rentgenstaru kristalogrāfiju noteiktajai ORF6 struktūrai ir ssDNS saistošiem proteīniem (SSB) raksturīga uzbūve ar oligonukleotīdu/oligosaharīdu saistīšanas folda domēnu un nestrukturētu C-terminālo segmentu. Par ORF6 piederību SSB saimei liecina arī strukturālā homoloģija ar fāga T7 SSB.

year	journal	country	edition	language
2014-01-01

https://dspace.lu.lv/dspace/handle/7/26914

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