Search results for " CAP"

Association between ribs shape and pulmonary function in patients with Osteogenesis Imperfecta

2019

Graphical abstract

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

2017

Growth phenotypes of microorganisms are a strong indicator of their underlying genetic fitness and can be segregated into 3 growth regimes: lag-phase, log-phase, and stationary-phase. Each growth phase can reveal different aspects of fitness that are related to various environmental and genetic conditions. High-resolution and quantitative measurements of all 3 phases of growth are generally difficult to obtain. Here we present a detailed method to characterize all 3 growth phases on solid media using an assay called One-cell Doubling Evaluation of Living Arrays of Yeast (ODELAY). ODELAY quantifies growth phenotypes of individual cells growing into colonies on solid media using time-lapse mi…

Tumor Heterogeneity, Single-Cell Sequencing, and Drug Resistance

2016

Tumor heterogeneity has been compared with Darwinian evolution and survival of the fittest. The evolutionary ecosystem of tumors consisting of heterogeneous tumor cell populations represents a considerable challenge to tumor therapy, since all genetically and phenotypically different subpopulations have to be efficiently killed by therapy. Otherwise, even small surviving subpopulations may cause repopulation and refractory tumors. Single-cell sequencing allows for a better understanding of the genomic principles of tumor heterogeneity and represents the basis for more successful tumor treatments. The isolation and sequencing of single tumor cells still represents a considerable technical ch…

Single-Cell Transcriptional Profiling of Cells Derived From Regenerating Alveolar Ducts

2020

Lung regeneration occurs in a variety of adult mammals after surgical removal of one lung (pneumonectomy). Previous studies of murine post-pneumonectomy lung growth have identified regenerative “hotspots” in subpleural alveolar ducts; however, the cell-types participating in this process remain unclear. To identify the single cells participating in post-pneumonectomy lung growth, we used laser microdissection, enzymatic digestion and microfluidic isolation. Single-cell transcriptional analysis of the murine alveolar duct cells was performed using the C1 integrated fluidic circuit (Fluidigm) and a custom PCR panel designed for lung growth and repair genes. The multi-dimensional data set was …

2020

Telomeres have the ability to adopt a lariat conformation and hence, engage in long and short distance intra-chromosome interactions. Budding yeast telomeres were proposed to fold back into subtelomeric regions, but a robust assay to quantitatively characterize this structure has been lacking. Therefore, it is not well understood how the interactions between telomeres and non-telomeric regions are established and regulated. We employ a telomere chromosome conformation capture (Telo-3C) approach to directly analyze telomere folding and its maintenance inS.cerevisiae. We identify the histone modifiers Sir2, Sin3 and Set2 as critical regulators for telomere folding, which suggests that a disti…

2020

Abstract A burgeoning body of literature suggests that poor childhood health leads to adverse health outcomes, lower educational attainment and weaker labour market outcomes in adulthood. We focus on an important but under-researched topic, which is the role played by infection-related hospitalization (IRH) in childhood and its links to labour market outcomes later in life. The participants aged 24–30 years in 2001 N = 1706 were drawn from the Young Finns Study, which includes comprehensive registry data on IRHs in childhood at ages 0–18 years. These data are linked to longitudinal registry information on labour market outcomes (2001–2012) and parental background (1980). The estimations wer…

Rachats d'actions et augmentation de capital : une analyse de leur combinaison appliquée au marché français

2013

Noting that over 22% of capital increases thrown at the French financial market are made when a share repurchase program is in progress, we propose an original study of the combination of the two operations. From 50 operations for the period 1999-2006, we test if the buybacks "prepare" the market before launching the capital increase or means to change the distribution of voting rights. Our results infirm these two hypotheses and confirm rather the proposition of a fortuitous concomitance.

An Easy Game? Experiences of ‘Homecoming’ in the Post-Socialist Context of Croatia and the Czech Republic

2016

The obstacles that often accompany remigration, planned and imagined as a ‘homecoming’, are seldom the topic of investigation in migration studies. Returning is not always an ‘easy game’. To explore this aspect of remigration, this chapter intends to focus on narratives of return produced mainly by so-called co-ethnic migrants who moved back to Croatia and the Czech Republic during the past two decades of post-socialist transformation. The empirical base of the chapter draws on the experiences and struggles accompanying remigration, and of arrival and acceptance in the respective society as described by returnees in biographical interviews. Attention is given to everyday social interaction …

Knowledge Sharing Among Tourists via Social Media: A Comparison Between Facebook and TripAdvisor

2016

This paper examines tourists' knowledge sharing behavior in social media. Based on social capital theory, we aim to examine the effects of three dimensions of social capital—structural (social interaction ties), cognitive (shared vision) and relational (trust)—for two different types of social media: Facebook and TripAdvisor. We propose a structural model that connects an antecedent (homophily) and a consequence (knowledge sharing through posting) of these main dimensions of social capital. An online survey is conducted with 1200 Spanish consumers. Based on the full sample, our structural equation modeling supports most of the hypothesized paths, while trust does not affect either social in…

Towards the realization of a basic professional profile model for Science, Technology and Mathematics (STEM) teachers

2017

[EN] In this work it is both discussed and provided a framework of speci c competences that may serve asa guide for setting up an ongoing process in the professional development of Science, Technology andMathematics (STEM) teachers. The fundamentals of the TPACK model serve to base the theoreticalbackground of this scheme, to which we have incorporated an additional feature which means to considerthe linguistic (L) dimension, thus transforming it into the TPACLK model. The di erent detailed profes-sional STEM capacities have been classi ed into six main sections. The two rst points discussed establishthe STEM disciplinary and didactic capacities; the third section corresponds to the role of…