Search results for " DNA"
showing 10 items of 2475 documents
Physical activity specifically evokes release of cell-free DNA from granulocytes thereby affecting liquid biopsy
2022
Clinical epigenetics 14, 29 (2022). doi:10.1186/s13148-022-01245-3
Reviewing the identity of the Maltese Polypodium (Polypodiaceae) – new evidence from morphology and flow cytometry
2020
The first record of Polypodium from Gozo (Maltese Islands) was described as a new endemic taxon, Polypodium vulgare subsp. melitense, based on its unique set of morphological characters. It was treated as a novelty and designated as a subspecies of P. vulgare mainly due to the lack of paraphyses, the presence of 10–16 annular cells, and a mean spore length of 64 μm. The fern was reassessed by us employing a more rigid morphological analysis and the application of flow cytometry. The absence of paraphyses was confirmed, but the number of annular cells (5–11) and the spore length (70–79 μm) differed from the previous study. These and other morphological traits, the phenology (leaf-shedding in…
Genetic Cell Ablation Reveals Clusters of Local Self-Renewing Microglia in the Mammalian Central Nervous System
2015
SummaryDuring early embryogenesis, microglia arise from yolk sac progenitors that populate the developing central nervous system (CNS), but how the tissue-resident macrophages are maintained throughout the organism’s lifespan still remains unclear. Here, we describe a system that allows specific, conditional ablation of microglia in adult mice. We found that the microglial compartment was reconstituted within 1 week of depletion. Microglia repopulation relied on CNS-resident cells, independent from bone-marrow-derived precursors. During repopulation, microglia formed clusters of highly proliferative cells that migrated apart once steady state was achieved. Proliferating microglia expressed …
Tenectin, a novel extracellular matrix protein expressed during Drosophila melanogaster embryonic development
2006
1567-133X (Print) Journal Article Research Support, Non-U.S. Gov't; During Drosophila embryonic development, various morphogenetic processes require the remodeling of the extracellular matrix. In a previous study, we have identified and characterized a cDNA encoding a novel putative extracellular matrix protein named tenebrin, in the beetle Tenebrio molitor. Here, we examine the expression of the Drosophila ortholog, referred to as Tenectin (Tnc), during embryonic development. Tnc is expressed in the majority of tissues of neuroectodermic origin such as hindgut, foregut, tracheal system, anal plate, and CNS. In the CNS, the Tnc transcript is restricted to a few cells, whereas the protein is…
GAL4-responsive UAS- tau as a tool for studying the anatomy and development of the Drosophila central nervous system
1997
To improve the quality of cytoplasmic labelling of GAL4-expressing cells in Drosophila enhancer-trap and transgenic strains, a new GAL4-responsive reporter UAS-tau, which features a bovine tau cDNA under control of a yeast upstream activation sequence (UAS), was tested. Tau, a microtubule-associated protein, is distributed actively and evenly into all cellular processes. Monoclonal anti-bovine Tau antibody reveals the axonal structure of the labelled cells with detail similar to that of Golgi impregnation. We demonstrate that the UAS-tau system is especially useful for studying processes of differentiation and reorganisation of identified neurones during postembryonic development.
Triatomine vectors of Trypanosoma cruzi: a molecular perspective based on nuclear ribosomal DNA markers.
2002
Chagas disease (American trypanosomiasis) is mainly transmitted by blood-sucking bugs of the reduviid subfamily Triatominae (Hemiptera: Prosorrhyncha). Control strategies are directed mainly against these insect vectors, as no vaccine is available and, except in the very early stage of infection, there is no effective chemotherapy. Studies of deoxyribonucleic acid (DNA) will lead to major advances in our knowledge of Triatominae and their relationships to Chagas disease transmission, epidemiology and control. Analyses of complete sequences of nuclear genes coding for ribosomal ribonucleic acid (rRNA) (rRNA genes) and spacers furnish significant information at the levels of higher taxons, ge…
Combination of molecular data support the existence of three main lineages in the phylogeny of aphids (Hemiptera: Aphididae) and the basal position o…
2010
Abstract The first molecular studies on the phylogeny of aphids (Hemiptera: Aphididae) bumped into a striking lack of phylogenetic structure for taxa levels higher than tribe, probably as a consequence of the rapid adaptive radiation that this group of insects went through during the Late Cretaceous. Here we present a new attempt to infer the relationships between major aphid taxa by the separate and combined analysis of two nuclear sequences (the long-wavelength opsin gene and the elongation factor 1α gene) and two mitochondrial sequences (the genes encoding the subunit 6 of the F-ATPase and the subunit II of the cytochrome oxidase). Our results confirm previous results with the grouping o…
Aliens Coming by Ships: Distribution and Origins of the Ocellated Skink Populations in Peninsular Italy
2022
The ocellated skink (Chalcides ocellatus) is a widespread lizard, naturally distributed between the Maghreb and coastal Pakistan, with few insular populations in the Mediterranean coastal area. Some populations of this species have also been recorded in peninsular Italy, Campania and Southern Tuscany due to accidental introductions via touristic and commercial routes. In this work, we conducted genetic analyses on mitochondrial DNA COXI, cytb and 16S mtDNA genes on a sample of Italian insular and peninsular populations. Differently from what previously suggested, the nucleus in Portici (Southern Italy) may have originated from Sardinia. The intense trade and touristic traffic between Sardin…
Nuevo registro y caracterización molecular de la tortuga verde Chelonia mydas (Testudines, Cheloniidae) en las aguas costeras sicilianas
2017
A juvenile specimen of the Green Turtle, Chelonia mydas, was recovered in Sicilian coastal waters close to Avola (Syracuse province, south-eastern Sicily, Italy). Before being released, the specimen was measured and inspected for the presence of ingested hooks or other possible harms to its health. A fragment of the mtDNA marker COI was amplified and sequenced in order to provide the first molecular data for the species from the central Mediterranean area. The possible influence of climate change on the occurrence of this thermophilic species in the central Mediterranean area is briefly discussed.
A new record and molecular characterization of the Green Turtle Chelonia mydas (Testudines, Cheloniidae) in Sicilian coastal waters
2017
A juvenile specimen of the Green Turtle, Chelonia mydas, was recovered in Sicilian coastal waters close to Avola (Syracuse province, south-eastern Sicily, Italy). Before being released, the specimen was measured and inspected for the presence of ingested hooks or other possible harms to its health. A fragment of the mtDNA marker COI was amplified and sequenced in order to provide the first molecular data for the species from the central Mediterranean area. The possible influence of climate change on the occurrence of this thermophilic species in the central Mediterranean area is briefly discussed.