Search results for " Stability"

Conformational equilibrium and intramolecular hydrogen bond of 4′X and 4X substituted 2′(OH)chalcones

1999

Abstract By using the AM1 method on 4X and 4′X substituted 2′(OH)chalcones (X=H, OH, F, Cl, CH 3 , CH 3 O, N(CH 3 ) 2 , and NO 2 ), certain currently unclear structural characteristics, conformational equilibria, intramolecular hydrogen bonds and UV spectroscopic properties had been clarified. The compounds studied have non-planar structures. The 2′(OH),4′Xchalcones only present the trans - s - cis conformation. trans - s - cis and trans - s - trans conformers of 2′(OH),4X-chalcones have a comparable thermodynamic stability. For these chalcones, a conformational equilibrium at 298 K exists, with 91% of the trans - s - cis form. The proposal of analyzing the intramolecular hydrogen bond of 2…

Binding properties of mono-6-amino-beta-cyclodextrin towards p-nitroaniline derivatives: a polarimetric study

Nuclear Translocation of Mismatch Repair Proteins MSH2 and MSH6 as a Response of Cells to Alkylating Agents

2000

Mammalian mismatch repair has been implicated in mismatch correction, the prevention of mutagenesis and cancer, and the induction of genotoxicity and apoptosis. Here, we show that treatment of cells specifically with agents inducing O(6)-methylguanine in DNA, such as N-methyl-N'-nitro-N-nitrosoguanidine and N-methyl-N-nitrosourea, elevates the level of MSH2 and MSH6 and increases GT mismatch binding activity in the nucleus. This inducible response occurs immediately after alkylation, is long-lasting and dose-dependent, and results from translocation of the preformed MutSalpha complex (composed of MSH2 and MSH6) from the cytoplasm into the nucleus. It is not caused by an increase in MSH2 gen…

Regulation of ribonucleotide reductase in response to iron deficiency

2011

Ribonucleotide reductase (RNR) is an essential enzyme required for DNA synthesis and repair. Although iron is necessary for class Ia RNR activity, little is known about the mechanisms that control RNR in response to iron deficiency. In this work, we demonstrate that yeast cells control RNR function during iron deficiency by redistributing the Rnr2–Rnr4 small subunit from the nucleus to the cytoplasm. Our data support a Mec1/Rad53-independent mechanism in which the iron-regulated Cth1/Cth2 mRNA-binding proteins specifically interact with the WTM1 mRNA in response to iron scarcity, and promote its degradation. The resulting decrease in the nuclear-anchoring Wtm1 protein levels leads to the re…

Gene expression is circular: factors for mRNA degradation also foster mRNA synthesis.

2013

SummaryMaintaining proper mRNA levels is a key aspect in the regulation of gene expression. The balance between mRNA synthesis and decay determines these levels. We demonstrate that most yeast mRNAs are degraded by the cytoplasmic 5′-to-3′ pathway (the “decaysome”), as proposed previously. Unexpectedly, the level of these mRNAs is highly robust to perturbations in this major pathway because defects in various decaysome components lead to transcription downregulation. Moreover, these components shuttle between the cytoplasm and the nucleus, in a manner dependent on proper mRNA degradation. In the nucleus, they associate with chromatin—preferentially ∼30 bp upstream of transcription start-sit…

c-Fos is required for excision repair of UV-light induced DNA lesions by triggering the re-synthesis of XPF

2006

Cells deficient in c-Fos are hypersensitive to ultraviolet (UV-C) light. Here we demonstrate that mouse embryonic fibroblasts lacking c-Fos (fos-/-) are defective in the repair of UV-C induced DNA lesions. They show a decreased rate of sealing of repair-mediated DNA strand breaks and are unable to remove cyclobutane pyrimidine dimers from DNA. A search for genes responsible for the DNA repair defect revealed that upon UV-C treatment the level of xpf and xpg mRNA declined but, in contrast to the wild type (wt), did not recover in fos-/- cells. The observed decline in xpf and xpg mRNA is due to impaired re-synthesis, as shown by experiments using actinomycin D. Block of xpf transcription resu…

Switching times in long-overlap Josephson junctions subject to thermal fluctuations and non-Gaussian noise sources

2014

We investigate the superconducting lifetime of long current-biased Josephson junctions, in the presence of Gaussian and non-Gaussian noise sources. In particular, we analyze the dynamics of a Josephson junction as a function of the noise signal intensity, for different values of the parameters of the system and external driving currents. We find that the mean lifetime of the superconductive state is characterized by nonmonotonic behavior as a function of noise intensity, driving frequency and junction length. We observe that these nonmonotonic behaviours are connected with the dynamics of the junction phase string during the switching towards the resistive state. An important role is played…

Interfacial water structure controls protein conformation.

2007

A phenomenological theory of salt-induced Hofmeister phenomena is presented, based on a relation between protein solubility in salt solutions and protein-water interfacial tension. As a generalization of previous treatments, it implies that both kosmotropic salting out and chaotropic salting in are manifested via salt-induced changes of the hydrophobic/hydrophilic properties of protein-water interfaces. The theory is applied to describe the salt-dependent free energy profiles of proteins as a function of their water-exposed surface area. On this basis, three classes of protein conformations have been distinguished, and their existence experimentally demonstrated using the examples of bacter…

A Boundary Element Formulation for Modelling Structural Health Monitoring Applications

2015

In this paper, a boundary element formulation for modelling pitch-catch damage detection applications is introduced. The current formulation has been validated by both finite element analyses and physical experiments. Comparing to the widely used finite element method, the current formulation does not only use less computational resources, but also demonstrates higher numerical stability. doi: 10.12783/SHM2015/221

Darcy–Forchheimer Magnetized Nanofluid flow along with Heating and Dissipation Effects over a Shrinking Exponential Sheet with Stability Analysis

2022

Nanoparticles have presented various hurdles to the scientific community during the past decade. The nanoparticles dispersed in diverse base fluids can alter the properties of fluid flow and heat transmission. In the current examination, a mathematical model for the 2D magnetohydrodynamic (MHD) Darcy–Forchheimer nanofluid flow across an exponentially contracting sheet is presented. In this mathematical model, the effects of viscous dissipation, joule heating, first-order velocity, and thermal slip conditions are also examined. Using similarity transformations, a system of partial differential equations (PDEs) is converted into a set of ordinary differential equations (ODEs). The problem is …