Search results for "APT"

PTC124-mediated translational readthrough of a nonsense mutation causing Usher syndrome type 1C.

2011

We investigated the therapeutic potential of the premature termination codon (PTC) readthrough-inducing drug PTC124 in treating the retinal phenotype of Usher syndrome, caused by a nonsense mutation in the USH1C gene. Applications in cell culture, organotypic retina cultures, and mice in vivo revealed significant readthrough and the recovery of protein function. In comparison with other readthrough drugs, namely the clinically approved readthrough-inducing aminoglycoside gentamicin, PTC124 exhibits significant better retinal biocompatibility. Its high readthrough efficiency in combination with excellent biocompatibility makes PTC124 a promising therapeutic agent for PTCs in USH1C, as well a…

(A,B) In vivo GCaMP6f signals recorded in layers M1, M5 and M9/10 of Mi1 (A) and Tm3 (B) neurons, before (blue, green) and after (gray, red) applicat…

2019

Sensory systems sequentially extract increasingly complex features. ON and OFF pathways, for example, encode increases or decreases of a stimulus from a common input. This ON/OFF pathway split is thought to occur at individual synaptic connections through a sign-inverting synapse in one of the pathways. Here, we show that ON selectivity is a multisynaptic process in the Drosophila visual system. A pharmacogenetics approach demonstrates that both glutamatergic inhibition through GluClα and GABAergic inhibition through Rdl mediate ON responses. Although neurons postsynaptic to the glutamatergic ON pathway input L1 lose all responses in GluClα mutants, they are resistant to a cell-type-specifi…

Hidalgo ja käsikirjoittaja : tekijän näkökulma adaptaatioon ja visuaaliseen kerrontaan laajassa sarjakuvassa

2006

Empathy, Entrainment, and Perceived Interaction in Complex Dyadic Dance Movement

2019

The current study explores how individuals' tendency to empathize with others (trait empathy) modulates interaction and social entrainment in dyadic dance in a free movement context using perceptual and computationally derived measures. Stimuli consisting of 24 point-light animations were created using motion capture data selected from a sample of 99 dyads, based on self-reported trait empathy. Individuals whose Empathy Quotient (EQ) scores were in the top or bottom quartile of all scores were considered to have high or low empathy, respectively, and twelve dyads comprised of four high-high, four low-low, and four high-low empathy combinations were identified. Animations of these dyads were…

Adaptētas galda spēles angļu valodas vārda krājuma apguvei 10. klasē

2017

Vārda krājuma bagātināšana ir viens no pamatuzdevumiem komunikatīvu prasmju attīstībai svešvalodā. Skolēniem var rasties grūtības angļu valodas vārdu izmantošanā runā un rakstos, un dažiem no viņiem trūkst motivācijas mācīties jaunus vārdus. Lai pārbaudītu, kā adaptētas galda spēles var veicināt angļu valodas vārda krājuma apguvi, tika veikts gadījuma pētījums. Pēc nodarbību cikla ar adaptēto galda spēļu izmantošanu tika veikta skolēnu zināšanu pārbaude. Kontroldarba rezultāti parādīja, ka mācīšanās ar adaptētu galda spēļu palīdzību veicināja augstu vārdu atpazīšanas un kontrolētas izmantošanas līmeni. Skolēnu mutvārdu uzstāšanās un rakstu darbu satura analīze atklāja, ka brīvā valodas liet…

Development of an immunochromatographic assay based on carbon nanoparticles for the determination of the phytoregulator forchlorfenuron

2013

Rapid analytical methods enabling the determination of diverse targets are essential in a number of research areas, from clinical diagnostics to feed and food quality and safety. Herein, the development of a quantitative immunochromatographic assay for the detection of the synthetic phytoregulator forchlorfenuron (CPPU) is described. The competitive lateral flow immunoassay (LFIA) was based on the immobilization onto a nitrocellulose membrane of an ovalbumin-CPPU conjugate (test line) and on the use of an immunodetection ligand consisting of carbon nanoparticles labeled with an anti-CPPU monoclonal antibody through interaction with a secondary antibody. The presence of CPPU in horticultural…

Videowikis for improved problem-based collaborative learning: Engaging information systems science students

2013

The contemporary era of social media and web 2.0 has enabled a bottom-up on-line collaborative approach with easy content creation and subsequent knowledge sharing. The technically literate students of today and the changes in pedagogy towards a user-centred approach, where learners engage in the learning process by constructing new ideas and concepts based on their current or past knowledge facilitate the use of social media in learning environments. This paper describes the combination of a wiki and screen capture videos as a complementary addition to conventional lectures in an information management and information systems development course. The basis for our approach was collaborative…

L’amica geniale in Inghilterra: il caso dell’adattamento radio di Timberlake Wertenbaker

2019

This paper takes into consideration Timberlake Wertenbaker's adaptation of Ferrante's My Brilliant Friend (2016) for the radio. In particular, the focus of the paper is on the first of the four radioplays and on the dramatic strategies used and adaptation choices made by Wertenbaker. We shall focus on the use of two main adult voices (Lena and Lila), the use of the voice over, and the representation of time. The chapter also considers the image of "neapolitanity" offered in the radioplay, the reception with the British audience with respect to the meridionist idea of Southern Italy as poor and violent. I aim to show how, with some exceptions, Wertenbaker somehow manages to push beyond the c…

Impact of Energy Availability, Health and Sex on Hemoglobin Mass Responses Following Live-High–Train-High Altitude Training in Elite Female and Male …

2018

Purpose: The authors investigated the effects of sex, energy availability (EA), and health status on the change in hemoglobin mass (ΔHbmass) in elite endurance athletes over ∼3–4 wk of live-high–train-high altitude training in Flagstaff, AZ (2135 m; n = 27 women; n = 21 men; 27% 2016 Olympians). Methods: Precamp and postcamp Hbmass (optimized carbon monoxide rebreathing method) and iron status were measured, EA was estimated via food and training logs, and a Low Energy Availability in Females Questionnaire (LEAFQ) and a general injury/illness questionnaire were completed. Hypoxic exposure (h) was calculated with low (600 h) groupings. Results: Absolute and relative percentage ΔHbmass was si…

Excess electronic recoil events in XENON1T

2020

We report results from searches for new physics with low-energy electronic recoil data recorded with the XENON1T detector. With an exposure of 0.65 t-y and an unprecedentedly low background rate of $76\pm2$ events/(t y keV) between 1 and 30 keV, the data enables sensitive searches for solar axions, an enhanced neutrino magnetic moment, and bosonic dark matter. An excess over known backgrounds is observed at low energies and most prominent between 2 and 3 keV. The solar axion model has a 3.4$\sigma$ significance, and a 3D 90% confidence surface is reported for axion couplings to electrons, photons, and nucleons. This surface is inscribed in the cuboid defined by $g_{ae}<3.8 \times 10^{-12}$,…