Search results for "Angioblast"

Ku factor is responsible of Hsp70 basal transcription in mouse mesoangioblasts

2008

H2O2 INDUCES NECROPTOSIS IN MESOANGIOBLAST STEM CELLS

2018

Stem cells are used in regenerative medicine, but their therapeutic efficacy is compromised by their huge death during the first days post-transplantation. Indeed, the microenvironment within damaged tissues is hostile for stem cell survival mainly due to oxidative stress. H2O2 may play a relevant role in inducing death of the injected cells. The aim of our study was to determine the mechanism of mesoangioblast (A6) cell death after an H2O2 treatment. FACS analysis with annV/PI showed that H2O2 induced a dose and time-dependent decrement in A6 viability. We have also found an increase in caspases 8, 9 and 3 activity after the treatment. To assess their involvement in cell death, the pan cas…

Mouse mesoangioblasts release Hsp70 in a controlled manner through membrane vesicle shedding

2009

Mesoangioblaststs (Mabs) are mesodermal stem cells associated with vessels which can differentiate into different mesoderm cell types. They have the property to cross endothelial barrier and when injected into circulation they localize into damaged tissues. A6 cells, a clone of mouse Mabs, express Hsp70 protein in physiological conditions and this synthesis may be required to answer to several intra- and/or extra-cellular needs. It was found that Hsp70 may be released outside from several type of cells, but its role remains still undefined. In order to clarify the reason of Hsp70 constitutive expression, we prepared several A6 clones with different levels of Hsp70 expression by stable RNA i…

A sub-population of mesoangioblasts displays features of resistance and proliferation confirmed by transcriptome analysis.

2014

Paracrine effect of membrane vesicles released by mouse mesoangioblast stem cells on non correlated cell types

2016

Introduction Mouse mesoangioblasts are vessel-associated multipotent progenitor stem cells, which are able to differentiate into different mesodermal cell types. In our previous paper we have demonstrated that these cells are able to shed in the extracellular environment membrane vesicles (EV), which contain both structural proteins and biological factors such as FGF2 and the two gelatinases MMP2/9. EV represent an important mediator of cell-to-cell communication and are involved in both autocrine and paracrine signalling. Interestingly, there is a bidirectional signalling exchange between stem cell EV and damaged cells. In particular, EV from injured cells can reprogram stem cells to acqui…

Heart infarct in NOD-SCID mice: therapeutic vasculogenesis by transplantation of human CD34+ cells and low dose CD34+KDR+ cells

2004

Hematopoietic (Hem) and endothelial (End) lineages derive from a common progenitor cell, the hemangioblast: specifically, the human cord blood (CB) CD34+KDR+ cell fraction comprises primitive Hem and End cells, as well as hemangioblasts. In humans, the potential therapeutic role of Hem and End progenitors in ischemic heart disease is subject to intense investigation. Particularly, the contribution of these cells to angiogenesis and cardiomyogenesis in myocardial ischemia is not well established. In our studies, we induced myocardial infarct (MI) in the immunocompromised NOD-SCID mouse model, and monitored the effects of myocardial transplantation of human CB CD34+ cells on cardiac function.…

High-density ZnO nanowires for cellular biointerfaces: a new role as myogenic differentiation switch

2019

The design of artificial platforms for expanding undifferentiated stem cells is of tremendous importance for regenerative medicine [1]. We have recently demonstrated that a ZnO nanowires (NWs) decorated glass support permits to obtain a differentiation switch during proliferation for mesoangioblasts (MABs)– i.e. multipotent progenitor cells which are remarkable candidates for the therapy of muscle diseases [2]. We have optimized the ZnO NWs synthesis on glass surfaces by numerical simulations and experimental systematic investigations, considering zinc speciation and supersaturation [3]. In particular, we demonstrated by numerical simulations that the ligand ethylenediamine, at the isoelect…

Mouse mesoangioblast behaviour when subjected to cellular stress

2009

Selezione di cloni più resistenti allo stress ossidativo dalla popolazione di cellule staminali mesoangioblasti

2012

Paracrine roles of extracellular vesicles released by mouse mesoangioblasts

2017

Extracellular vesicles (EV) represent an important mediator of cell-to-cell communication and are involved in both autocrine and paracrine signaling, with a critical role in a number of physiological and pathological conditions.1 The bioactive molecules contained within EV simultaneously activate several different pathways resulting in the synergistic stimulation of target cells. The discovery and characterization of EV have added a novel understanding to regenerative medicine, namely the finding that stem cells are an abundant source of EV.1-2 A6 mouse mesoangioblasts, vessel-associated multipotent progenitor stem cells that are capable of differentiating into different mesodermal cell typ…