Search results for "BIOS"
showing 10 items of 2557 documents
Transcriptional responses of Medicago truncatula upon sulfur deficiency stress and arbuscular mycorrhizal symbiosis
2014
International audience; Sulfur plays an essential role in plants' growth and development and in their response to various abiotic and biotic stresses despite its leachability and its very low abundance in the only form that plant roots can uptake (sulfate). It is part of amino acids, glutathione (GSH), thiols of proteins and peptides, membrane sulfolipids, cell walls and secondary products, so reduced availability can drastically alter plant growth and development. The nutritional benefits of symbiotic interactions can help the plant in case of S deficiency. In particular the arbuscular mycorrhizal (AM) interaction improves N, P and S plant nutrition, but the mechanisms behind these exchang…
Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis
2013
International audience; The mutualistic symbiosis involving Glomeromycota, a distinctive phylum of early diverging Fungi, is widely hypothesized to have promoted the evolution of land plants during the middle Paleozoic. These arbuscular mycorrhizal fungi (AMF) perform vital functions in the phosphorus cycle that are fundamental to sustainable crop plant productivity. The unusual biological features of AMF have long fascinated evolutionary biologists. The coenocytic hyphae host a community of hundreds of nuclei and reproduce clonally through large multinucleated spores. It has been suggested that the AMF maintain a stable assemblage of several different genomes during the life cycle, but thi…
Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula
2004
To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…
Acclimatised rumen culture for raw microalgae conversion into biogas: Linking microbial community structure and operational parameters in anaerobic m…
2019
[EN] Ruminal fluid was inoculated in an Anaerobic Membrane Reactor (AnMBR) to produce biogas from raw Scenedesmus. This work explores the microbial ecology of the system during stable operation at different solids retention times (SRT). The 16S rRNA amplicon analysis revealed that the acclimatised community was mainly composed of Anaerolineaceae, Spirochaetaceae, Lentimicrobiaceae and Cloacimonetes fermentative and hydrolytic members. During the highest biodegradability achieved in the AnMBR (62%) the dominant microorganisms were Fervidobacterium and Methanosaeta. Different microbial community clusters were observed at different SRT conditions. Interestingly, syntrophic bacteria Gelria and …
Implications of the EFSA Scientific Opinion on Site Directed Nucleases 1 and 2 for Risk Assessment of Genome-Edited Plants in the EU
2021
Genome editing is a set of techniques for introducing targeted changes in genomes. It may be achieved by enzymes collectively called site-directed nucleases (SDN). Site-specificity of SDNs is provided either by the DNA binding domain of the protein molecule itself or by RNA molecule(s) that direct SDN to a specific site in the genome. In contrast to transgenesis resulting in the insertion of exogenous DNA, genome editing only affects specific endogenous sequences. Therefore, multiple jurisdictions around the world have exempted certain types of genome-edited organisms from national biosafety regulations completely, or on a case-by-case basis. In the EU, however, the ruling of the Court of J…
Tackling invasive alien species in Europe II: threats and opportunities until 2020
2017
2nd Freshwater Invasives - Networking for Strategy II (FINS-II) Conference -- JUL 11-14, 2016 -- Zagreb, CROATIA Skora, Michal Edward/0000-0002-9121-1318; Groom, Quentin/0000-0002-0596-5376; Lukas, Juliane/0000-0003-3336-847X; Skora, Michal/0000-0002-9121-1318; Piria, Marina/0000-0001-6324-9282; Smith, Emily/0000-0003-2767-9933; Simonovic, Predrag/0000-0002-4819-4962; Pastorino, Paolo/0000-0002-0585-1168; Koutsikos, Nicholas/0000-0003-0680-4504; Vilizzi, Lorenzo/0000-0001-8103-885X; Dobrzycka-Krahel, Aldona/0000-0002-4252-895X; Tarkan, Ali Serhan/0000-0001-8628-0514 WOS: 000412582000002 Invasive alien species (IAS) are a significant and growing problem worldwide. In Europe, some aspects of …
Female mate choice in convict cichlids is transitive and consistent with a self-referent directional preference
2013
10 pages; International audience; INTRODUCTION: One of the most important decisions that an animal has to make in its life is choosing a mate. Although most studies in sexual selection assume that mate choice is rational, this assumption has not been tested seriously. A crucial component of rationality is that animals exhibit transitive choices: if an individual prefers option A over B, and B over C, then it also prefers A over C. RESULTS: We assessed transitivity in mate choice: 40 female convict cichlids had to make a series of binary choices between males of varying size. Ninety percent of females showed transitive choices. The mean preference index was significantly higher when a female…
Parasite-induced behavioral change: mechanisms.
2010
Animal behavior and parasitism are more tightly linked than commonly thought. One of the most astonishing phenomena in host–parasite antagonistic interactions is ‘host manipulation,’ that is, the ability of a parasite to alter the behavior of its host in ways that appear to increase parasite fitness at the expense of host fitness. The mechanisms by which a parasite hijacks the behavior of its host have been explored using ethopharmacological and immunocytochemical approaches or carrying out a large-scale proteomic study on manipulated host’s brain. These few mechanistic studies have confirmed both the complexity of host manipulation by parasites and the importance of understanding the molec…
Effects of Arbuscular Mycorrhizal Fungi on the Vegetative Vigor of Ailanthus altissima (Mill.) Swingle Seedlings under Sustained Pot Limitation
2018
In order to invade new ecosystems, invasive alien plants need to cope with different microbial communities. Whilst the ability to avoid antagonists is well recognized, the opportunity to establish mutualistic associations is less known, even in widespread invasive species such as Ailanthus altissima (Mill.) Swingle. We sought to evaluate whether the beneficial effects of arbuscular mycorrhizal fungi (AMF) on Ailanthus seedlings are maintained over time, under prolonged pot limitation. We compared three-month-, three-year- and four-year-old mycorrhizal seedlings grown in natural forest soil (NT) with seedlings grown in sterilized (ST) and non-mycorrhizal (NM) soils, in pots of 3.4 L (22 &tim…
25S rDNA-based molecular monitoring of glomalean fungi in sewage sludge-treated field plots
2001
Recycling of sewage wastes in agriculture is likely to affect the biological activity of soils through contamination of ecosystems by pathogens and metallic or organic micropollutants. The impact of sewage sludge spreading under field conditions on arbuscular mycorrhiza (AM) formation by a community of glomalean fungi was evaluated using a nested polymerase chain reaction (PCR) and discriminating primers based on 25S rDNA polymorphisms to detect different fungal species within root systems. Medicago truncatula was grown in soil of field plots amended or not with a composted sewage sludge, spiked or not with organic or metallic micropollutants. Overall AM development in roots decreased with …