Search results for "ECL"
showing 10 items of 1262 documents
A new mathematical approach for the estimation of the AUC and its variability under different experimental designs in preclinical studies
2011
The aim of the present work was to develop a new mathematical method for estimating the area under the curve (AUC) and its variability that could be applied in different preclinical experimental designs and amenable to be implemented in standard calculation worksheets. In order to assess the usefulness of the new approach, different experimental scenarios were studied and the results were compared with those obtained with commonly used software: WinNonlin® and Phoenix WinNonlin®. The results do not show statistical differences among the AUC values obtained by both procedures, but the new method appears to be a better estimator of the AUC standard error, measured as the coverage of 95% confi…
Multicellular tumor spheroids: an underestimated tool is catching up again.
2009
The present article highlights the rationale, potential and flexibility of tumor spheroid mono- and cocultures for implementation into state of the art anti-cancer therapy test platforms. Unlike classical monolayer-based models, spheroids strikingly mirror the 3D cellular context and therapeutically relevant pathophysiological gradients of in vivo tumors. Some concepts for standardization and automation of spheroid culturing, monitoring and analysis are discussed, and the challenges to define the most convenient analytical endpoints for therapy testing are outlined. The potential of spheroids to contribute to either the elimination of poor drug candidates at the pre-animal and pre-clinical …
Preeclampsia: a defect in decidualization is associated with deficiency of Annexin A2.
2020
Background Decidualization defects in the endometrium have been demonstrated at the time of delivery in women with severe preeclampsia and to linger for years, which suggests a maternal contribution to the pathogenesis of this condition. Global transcriptional profiling reveals alterations in gene expression, which includes down-regulation of Annexin A2 in severe preeclampsia patients with decidualization resistance. Objective We investigated the functional role of Annexin A2 deficiency during endometrial decidualization and its potential contribution to shallow trophoblast invasion during implantation and subsequent placentation using in vitro and in vivo modeling. Study Design Annexin A2 …
Digital Image Analysis Applied to Tumor Cell Proliferation, Aggressiveness, and Migration-Related Protein Synthesis in Neuroblastoma 3D Models
2020
Patient-derived cancer 3D models are a promising tool that will revolutionize personalized cancer therapy but that require previous knowledge of optimal cell growth conditions and the most advantageous parameters to evaluate biomimetic relevance and monitor therapy efficacy. This study aims to establish general guidelines on 3D model characterization phenomena, focusing on neuroblastoma. We generated gelatin-based scaffolds with different stiffness and performed SK-N-BE(2) and SH-SY5Y aggressive neuroblastoma cell cultures, also performing co-cultures with mouse stromal Schwann cell line (SW10). Model characterization by digital image analysis at different time points revealed that cell pro…
Conclusion: University Ambiguities and Analytic Eclecticism
2018
This volume has examined six cases of university engagement in peripheral regions. While these regions have often been overlooked in the mainstream literature on university-region dynamics because they do not readily offer up success stories, they do facilitate an exploration into the challenges and difficulties that arise at the intersection of the university and region. Beginning with a theory rooted in institutionalist literature that depicts the university as a set of five ambiguities rather than as a coherent whole, the chapters have sought to apply the ambiguities of intention, causality, history, structure, and meaning to their regional context. In this conclusion, we pull together a…
RETA A TU MENTE, DESAFÍA A TU CEREBRO: COMPLEJIDAD AMBIENTAL Y SALUD CEREBRAL
2016
Abstract.According to the World Health Organization, it is estimated that more than 30 million people suffer from Alzheimer’s disease in the world. Currently, there is great interest in developing strategies to counteract the cognitive decline that accompanies aging and, to a greater extent, neurodegenerative diseases. The aim of the current study is to analyze the experimental evidence supporting the hypothesis that interventions based on novelty and complexity are more effective in order to achieve successful aging. In animal models it has shown that enriched environments and performing voluntary exercise can improve performance in learning and memory tasks, decrease anxiety response and …
Polymeric drug delivery micelle-like nanocarriers for pulmonary administration of beclomethasone dipropionate
2017
In this paper, the potential of novel polymeric micelles as drug delivery systems for Beclomethasone Dipropionate (BDP) administration into the lung is investigated. These nanostructures are obtained starting from α,β-poly(N-2-hydroxyethyl)-D,L-aspartamide (PHEA), which was subsequently functionalized with O-(2-aminoethyl)-Oâ-methylpolyethylenglycole (PEG2000), ethylenediamine (EDA) and lipoic acid (LA), obtaining PHEA-PEG2000-EDA-LA graft copolymer. Empty and drug-loaded micelles possess adequate chemical-physical characteristics for pulmonary administration such as spherical shape, slightly positive surface charge and mean size of about 200 nm. Besides, BDP-loaded micelles, obtained …
Education for Sustainable Development: A University Perspective
2021
Universities have a number of functions in developing Education for Sustainable Development (ESD): conducting study/teaching process themselves, participation in the development of study materials for other fields of studies, involvement in the development of study methods, as well as a responsibility for Sustainable Development Science. An equally important role of universities in the ESD process is the “training of trainers”—preparation of new teachers, who are aware of the need to promote the sustainable development process, are equipped with a systemic understanding of processes in the society and natural world, and know the local culture and traditions. The Aichi-Nagoya Declaration on …
THE HUMAN RIGHT TO HEALTH: A RETROSPECTIVE ANALYSIS AFTER 70 YEARS OF INTERNATIONAL RECOGNITION
2018
In 1948, the General Assembly of the United Nations adopted the first international text recognising universal human rights for all; the Universal Declaration of Human Rights. Article 25 recognises the right to an adequate standard of living, which includes the right to health and medical care. On the occasion of the 70th anniversary of the Declaration, this article presents an overview of the main developments that have been made towards understanding the content and implications of the right to health, as well as an analysis of some specific advancements that aim to facilitate the enforcement thereof. These include: a) the implication of private entities as responsible for right to health…
Tecla Navarra Masi (1892-1936): una giovane siciliana nel catalogo di Piero Gobetti
2021
Tecla Navarra Masi, giovane scrittrice siciliana, prematuramente scomparsa, è stata rappresentativa di quella cultura siciliana che, agli inizi del XX secolo, tenta di uscire dal ristretto ambito regionale. Testimonianza ne è l'inserimento e la pubblicazione di uno dei suoi saggi presso la casa editrice di Torino, fondata e diretta da Piero Gobetti. Tecla Navarra Masi, a young Sicilian writer, who died prematurely, was representative of that Sicilian culture which, at the beginning of the twentieth century, tried to escape from the restricted regional context. Testimony is the inclusion and publication of one of his essays at the Turin publishing house, founded and directed by Piero Gobetti.