Search results for "G2"

Role and importance of polymorphisms with respect to DNA methylation for the expression of CYP2E1 enzyme

2014

Different individuals possess slightly different genetic information and show genetically-determined differences in several enzyme activities due to genetic variability. Following an integrated approach, we studied the polymorphisms and methylation of sites contained in the 5' flanking region of the metabolizing enzyme CYP2E1 in correlation to its expression in both tumor and non-neoplastic liver cell lines, since to date little is known about the influence of these (epi)genetic elements in basal conditions and under induction by the specific inductor and a demethylating agent. In treated cells, reduced DNA methylation, assessed both at genomic and gene level, was not consistently associate…

Parvovirus B19 Genotype Specific Amino Acid Substitution in NS1 Reduces the Protein's Cytotoxicity in Culture

2010

A clinical association between idiopathic liver disease and parvovirus B19 infection has been observed. Fulminant liver failure, not associated with other liver-tropic viruses, has been attributed to B19 in numerous reports, suggesting a possible role for B19 components in the extensive hepatocyte cytotoxicity observed in this condition. A recent report by Abe and colleagues (Int J Med Sci. 2007;4:105-9) demonstrated a link between persistent parvovirus B19 genotype I and III infection and fulminant liver failure. The genetic analysis of isolates obtained from these patients demonstrated a conservation of key amino acids in the nonstructural protein 1 (NS1) of the disease-associated genotyp…

German bank lending to industrial and non-industrial countries: driven by fundamentals or different treatment?

2005

This paper shows that the substantial disparity in German bank lending towards industrial (IC) and non-industrial (Non-IC) countries is largely explained by differences in countries' endowments and only to a minor extent by German banks' different treatment of these country groups. This is demonstrated by applying a decomposition technique to an augmented gravity model that is estimated for German foreign lending using a new micro panel data-set on individual claims from the Deutsche Bundesbank covering the period from 1996 to 2002.

POLYMERIC FLUORESCENT NANOPARTICLES BASED ON A POLYASPARTAMIDE FOR IMAGING APPLICATIONS: EVALUATION OF GALACTOSE TARGETING ON HEPG2 CELL INTERNALIZAT…

2014

Biological properties of carotenoids extracted from Halobacterium halobium isolated from a Tunisian solar saltern

2013

Abstract Background Bioactive molecules have received increasing attention due to their nutraceutical attributes and anticancer, antioxidant, antiproliferative and apoptosis-inducing properties. This study aimed to investigate the biological properties of carotenoids extracted from Archaea. Methods Halophilic Archaea strains were isolated from the brine of a local crystallizer pond (TS7) of a solar saltern at Sfax, Tunisia. The most carotenoid-producing strain (M8) was investigated on heptoma cell line (HepG2), and its viability was assessed by the MTT-test. The cells were incubated with different sub-lethal extract rates, with carotenoid concentrations ranging from 0.2 to 1.5 μM. Antioxida…

Bu2Sn(N-acetyl-L-cysteinate) antitumor activity on HepG2 cells

2010

Comparative cytotoxic study of silica materials functionalised with essential oil components in HepG2 cells

2020

[EN] This work evaluated the cytotoxic effect of different EOCs-functionalised silica particle types. The in vitro toxicity of eugenol and vanillin-immobilised SAS, MCM-41 microparticles and MCM-41 nanoparticles was evaluated on HepG2 cells, and compared to free EOCs and pristine materials. The results revealed that free essential oil components and bare silica had a mild cytotoxic effect on HepG2 cells. However, the comparative study showed that free eugenol and vanillin had a milder cytotoxic effect than the equivalent concentrations of immobilised components on the different silica particles, while differences in cell viability between the bare and functionalised particles relied on the …

Efectos tóxicos de la zearalenona y sus metabolitos en líneas celulares

2017

La zearalenona (ZEA) y sus metabolitos α-zearalenol (α-ZOL) y β-zearalenol (β-ZOL), son metabolitos secundarios de hongos del genero Fusarium. La ZEA se absorbe y se metaboliza a α-ZOL y β-ZOL. Tanto la ZEA como sus metabolitos producen sus efectos tóxicos por la interacción con los receptores endocrinos aunque no es el único mecanismo por el cual pueden producir toxicidad. Se sabe que, además de disruptores endocrinos la ZEA y sus metabolitos son inmunotóxicos, hepatotóxicos y hematotóxicos causando alteraciones en el sistema reproductivo. La citotoxicidad de la ZEA y sus metabolitos ha sido determinada por distintos tiempos de exposición en diferentes células de mamíferos obteniéndose dif…

The human liver fatty acid binding protein (FABP1) gene is activated by FOXA1 and PPARα; and repressed by C/EBPα: Implications in FABP1 down-regulati…

2013

Liver fatty acid binding protein (FABP1) prevents lipotoxicity of free fatty acids and regulates fatty acid trafficking and partition. Our objective is to investigate the transcription factors controlling the human FABP1 gene and their regulation in nonalcoholic fatty liver disease (NAFLD). Adenovirus-mediated expression of multiple transcription factors in HepG2 cells and cultured human hepatocytes demonstrated that FOXA1 and PPARα are among the most effective activators of human FABP1, whereas C/EBPα is a major dominant repressor. Moreover, FOXA1 and PPARα induced re-distribution of FABP1 protein and increased cytoplasmic expression. Reporter assays demonstrated that the major basal activ…

N=2 topological gauge theory, the Euler characteristic of moduli spaces, and the Casson invariant

1991

We discuss gauge theory with a topological N=2 symmetry. This theory captures the de Rham complex and Riemannian geometry of some underlying moduli space $\cal M$ and the partition function equals the Euler number of $\cal M$. We explicitly deal with moduli spaces of instantons and of flat connections in two and three dimensions. To motivate our constructions we explain the relation between the Mathai-Quillen formalism and supersymmetric quantum mechanics and introduce a new kind of supersymmetric quantum mechanics based on the Gauss-Codazzi equations. We interpret the gauge theory actions from the Atiyah-Jeffrey point of view and relate them to supersymmetric quantum mechanics on spaces of…