Search results for "Gel"

showing 10 items of 3561 documents

A Direct Comparison of Seprafilm, Adept, Intercoat, and Spraygel for Adhesion Prophylaxis

2010

Background Commercially available agents for adhesion prophylaxis are legion but there is a lack of direct comparisons between them. Here we compare four of the most commonly used adhesion barriers against a control group in a clinically relevant rat model. Material and Methods Standardized lesions were created in Wistar rats using electrocautery and suturing. Subsequently, the experimental lesions were treated with Seprafilm (n = 30), Adept (n = 30), Intercoat (n = 30), Spraygel (n = 30), or no barrier (n = 30). The resulting adhesions were examined 14 d postoperatively. Results The mean area covered by adhesion was 77% in the control group, 46% in animals treated with Seprafilm, 54% in an…

medicine.medical_specialtySuturesbusiness.industryCauteryRat modelAdhesion (medicine)Tissue AdhesionsAdeptmedicine.diseaseIcodextrinRatsSurgeryGlucoseModels AnimalCell AdhesionmedicineAnimalsWounds and InjuriesSurgeryHyaluronic AcidRats WistarbusinessGelsGlucansJournal of Surgical Research
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Identification of Insulin in Chick Embryo Retina During Development and Its Inhibitory Effect on DNA Synthesis

1992

Incubation of chick embryo retinal explants with insulin resulted in a pronounced inhibition of thymidine uptake and incorporation into trichloroacetic acid-insoluble fraction. The inhibitory effect was highest with explants from embryos at day 7 and day 8, and thereafter it declined markedly with the age of embryos until day 11. A time-course study of the effect revealed that the inhibition occurred after a lag time; both thymidine uptake and incorporation were not altered significantly after 2-6 h of incubation with insulin, but began to decrease thereafter, reaching the maximum after 16 h. The effect was also dose dependent. After 16 h of incubation, the maximal inhibition (65%) was foun…

medicine.medical_specialtyThymidine kinase activitymedicine.medical_treatmentRadioimmunoassayChick EmbryoBiologyThymidine KinaseBiochemistryRetinaEmbryonic and Fetal DevelopmentCellular and Molecular Neurosciencechemistry.chemical_compoundInternal medicinemedicineAnimalsInsulinUridineIncubationChromatography High Pressure LiquidPancreatic hormoneDNA synthesisInsulinEmbryoRetinalDNAGlutamineEndocrinologychemistryChromatography GelThymidineJournal of Neurochemistry
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Localization of the bradykinin B2 receptor in uterus, bladder and Madin-Darby canine kidney cells

1997

Kinins are biologically active peptides that act through specific receptors, B1 and B2. Here we describe the localization of the bradykinin B2 receptor in Madin-Darby canine kidney cells and in the uterus and urinary bladder of rat or human origin. We discuss the suitability of anti-peptide antibodies to assess the tissue distribution of bradykinin B2 receptors.

medicine.medical_specialtyTissue FixationReceptor Bradykinin B2Urinary BladderUterusBradykininKidneyRadioligand Assaychemistry.chemical_compoundDogsAntibody SpecificityInternal medicineTumor Cells CulturedmedicineAnimalsHumansTissue DistributionReceptorPharmacologyKidneyParaffin EmbeddingUrinary bladderbiologyurogenital systemReceptors BradykininUterusImmunohistochemistryRadioligand AssayRatsmedicine.anatomical_structureEndocrinologyMicroscopy Fluorescencechemistrybiology.proteinCancer researchAutoradiographyImmunohistochemistryElectrophoresis Polyacrylamide GelFemaleAntibodyCell DivisionImmunopharmacology
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Sustained safety and efficacy of ligelizumab in patients with chronic spontaneous urticaria: A one‐year extension study

2021

Background: Ligelizumab, a next-generation, humanized anti-immunoglobulin E (IgE) monoclonal antibody is in development as a treatment for patients with chronic spontaneous urticaria, whose symptoms are inadequately controlled with standard-of-care therapy. Objective: To evaluate the long-term safety and re-treatment efficacy of ligelizumab 240 mg in patients who completed the core study and extension study. Methods: This open-label, single-arm, long-term Phase 2b extension study was designed to assess patients who were previously administered various doses of ligelizumab, omalizumab or placebo in the Phase 2b, dose-finding core study and who presented with active disease after Week 32. In …

medicine.medical_specialtyUrticariaImmunologyLigelizumab610OmalizumabOmalizumabImmunoglobulin EPlaceboAntibodies Monoclonal HumanizedInternal medicineActive diseasemedicineImmunology and AllergyHumansIn patientChronic UrticariaAdverse effectbiologybusiness.industryExtension studyChronic spontaneous urticariaTreatment OutcomeLigelizumabbiology.proteinIgEbusiness600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheitmedicine.drug
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Local Formula with Mucoadhesive Property: A Randomized Clinical Trial of a Therapeutic Agent for the Treatment of Oral Aphthous Ulcers

2020

Background Recurrent or occasional aphthous lesions represent a painful oral condition with high prevalence. Since the etiology is still unclear and most likely related to a dysfunction in the local immune system, several treatment strategies have been proposed, including systemic agents, local agents, and laser therapy, to reduce the pain and discomfort for the patient without acting on the causes. Materials and methods The purpose of the present randomized study was to assess the clinical efficacy of a new topical gel with mucoadhesive property to reduce the pain and the dimension of the aphthosis lesions. Fifty patients presenting at least one minor ulcer were randomized to a control gro…

medicine.medical_specialtyVisual analogue scalePainPlacebolaw.invention03 medical and health sciencesOral aphthous ulcers0302 clinical medicineRandomized controlled triallawAdjuvant therapyHumansMedicineClinical significanceMedical prescriptionGeneral DentistryWound Healingbusiness.industry030206 dentistryDermatologyTreatment Outcome030220 oncology & carcinogenesisEtiologyStomatitis AphthousbusinessGelsThe Journal of Contemporary Dental Practice
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Regulation by oestrogens of tachykinin NK3 receptor expression in the rat uterus.

1997

The expression of the tachykinin NK3 receptor and its regulation by ovarian steroids were analyzed by reverse transcription-polymerase chain reaction (RT-PCR) in uteri from ovariectomized rats. A single transcript corresponding to the 325-bp product expected for the tachykinin NK3 receptor was detected in uteri from olive oil-treated (control) ovariectomized rats. The level of tachykinin NK3 receptor mRNA in progesterone-treated animals was similar to that observed in uteri from control ones. Tachykinin NK3 receptor mRNA levels were significantly smaller in uteri from oestrogen-treated ovariectomized rats, with approximately a 32-fold decrease. These findings suggest that oestrogen, but not…

medicine.medical_specialtyanimal structuresNk3 receptorDNA Complementarymedicine.drug_classOvariectomyUterusBiologydigestive systemcomplex mixturesPolymerase Chain ReactionInternal medicineGene expressionmedicineAnimalsRNA MessengerRats WistarProgesteronePharmacologyElectrophoresis Agar GelMessenger RNAurogenital systemmusculoskeletal neural and ocular physiologyUterusEstrogensReceptors Neurokinin-3RatsEndocrinologymedicine.anatomical_structureGene Expression RegulationEstrogenIn uteroRat uterusOvariectomized ratFemaleEuropean journal of pharmacology
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Evaluation of early and late histamine release by three standard volume substitutes

2001

Following meta-analysis [1], the use of colloidal solutions in intensive care medicine is under discussion. Although polygeline [2] and human albumin are known to cause histamine release, other substances eg hydroxyethyl starch were thought to be without effect. Histamine release depends on the clinical scenario, concomitant drugs, infusion speed, duration and amount of infusion. However, there is no information about their effect regarding time periods longer than immediate reactions. This study investigated the histamine releasing effects of three plasma substitutes administered and observed over a longer period of time, simulating many of the clinical scenarios.

medicine.medical_specialtybusiness.industryHuman albuminPharmacologyHydroxyethyl starchCritical Care and Intensive Care MedicinePlasma Substituteschemistry.chemical_compoundchemistryConcomitantPolygelineEmergency medicineMeeting AbstractmedicinebusinessClinical scenarioHistaminemedicine.drugCritical Care
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Protective effects of plasma replacement fluids on erythrocytes exposed to mechanical stress

2000

Haemoglobin release from 40 suspensions of packed red blood cells in modified fluid gelatin, 4% albumin solution, 6% hydroxyethyl starch and normal saline was investigated in vitro during circulation with a roller pump from a heart-lung machine for 120 min at a flow rate of 2.5 l.min-1 at room temperature. The lowest haemoglobin release was obtained with erythrocytes in modified fluid gelatin, whereas free haemoglobin concentrations became progressively higher with albumin, hydroxyethyl starch and normal saline [median free haemoglobin (interquartile range) after 120 min circulation: gelatin 493 (360-601) mg.l-1, albumin 692 (590-1111) mg.l-1, hydroxyethyl starch 1121 (692-1518) mg.l-1, nor…

medicine.medical_specialtyfood.ingredientChromatographybusiness.industrymedicine.medical_treatmentExtracorporeal circulationAlbuminHydroxyethyl starchmedicine.diseaseHaemolysisGelatinHemolysisSurgeryRed blood cellAnesthesiology and Pain Medicinefoodmedicine.anatomical_structureMedicinebusinessSalinemedicine.drugAnaesthesia
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Human antiphospholipid antibodies induce TNFα in monocytes via Toll-like receptor 8

2009

The antiphospholipid syndrome (APS) is characterized by recurrent arterial and/or venous thromboses, pregnancy loss and the presence of antiphospholipid antibodies (aPL). One of the discussed mechanisms of this thrombotic activity in APS patients is attributed to TNFalpha secretion in monocytes after aPL stimulation. To investigate this mechanism in detail, we employed a monoclonal aPL and IgG fractions of APS patients for stimulation of human peripheral monocytes. Stimulation with this monoclonal aPL resulted in an increased expression and secretion of TNFalpha, caused by specific upregulation of TLR8 mRNA and protein expression levels. To confirm the specificity of this finding we could d…

medicine.medical_specialtymedicine.drug_classBlotting WesternImmunologyEnzyme-Linked Immunosorbent AssayStimulationCell SeparationBiologyMonoclonal antibodyPeripheral blood mononuclear cellMonocytesProinflammatory cytokineDownregulation and upregulationimmune system diseasesAntiphospholipid syndromeInternal medicinemedicineHumansImmunology and AllergyReverse Transcriptase Polymerase Chain ReactionTumor Necrosis Factor-alphaAntibodies MonoclonalHematologyAntiphospholipid SyndromeFlow Cytometrymedicine.diseaseEndocrinologyToll-Like Receptor 8MonoclonalImmunologyAntibodies AntiphospholipidElectrophoresis Polyacrylamide GelTumor necrosis factor alphaImmunobiology
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Impact of single-dose application of TGF-β, copper peptide, stanozolol and ascorbic acid in hydrogel on midline laparatomy wound healing in a diabeti…

2012

Despite numerous advances and improvements in surgical techniques the incidence of incisional hernias after laparotomy remains high. The aim of this study was to investigate possible effects of single application of ascorbic acid, stanozolol, a synthetic anabolic steroid, copper peptide and transforming growth factor-β (TGF-β) on laparotomy wound healing in an incisional wound model in diabetic mice. After diabetes induction with streptozotozin in Balb-c mice, midline laparatomies were carried out. Closure of the linea alba was followed by single-dose application of the agents dissolved in a hydrogel before skin closure. The functional outcome was assessed in terms of maximum tensile streng…

medicine.medical_specialtymedicine.medical_treatmentFibrillar CollagensAscorbic AcidBiologyDiabetes Mellitus ExperimentalCollagen Type IIICicatrixMiceTransforming Growth Factor betaInternal medicineDiabetes mellitusLaparotomyTensile StrengthGeneticsmedicineAnimalsStanozololLaparotomyMice Inbred BALB CWound HealingHydrogelsGeneral MedicineAscorbic acidmedicine.diseaseDisease Models Animalmedicine.anatomical_structureEndocrinologyLinea alba (abdomen)FemaleWound healingPeptidesAnabolic steroidCopperStanozololmedicine.drugInternational journal of molecular medicine
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