Search results for "Laboratory"
showing 10 items of 923 documents
Primary Cytomegalovirus Infection in Seronegative Kidney Transplant Patients Is Associated with Protracted Cold Ischemic Time of Seropositive Donor O…
2017
Human Cytomegalovirus (CMV) can lead to primary infection or reactivation in CMV-seronegative or -seropositive kidney transplant recipients, respectively. Complications comprise severe end-organ diseases and acute or chronic transplant rejection. Risk for CMV manifestation is stratified according to the CMV-IgG-serostatus, with donor+/recipient- (D+/R-) patients carrying the highest risk for CMV-replication. However, risk factors predisposing for primary infection in CMV-seronegative recipients are still not fully elucidated. Therefore, we monitored D+/R- high-risk patients undergoing kidney transplantation in combination with antiviral prophylaxis for the incidence of CMV-viremia for a med…
STR analysis of artificially degraded DNA--results of a collaborative European exercise.
2003
Degradation of human DNA extracted from forensic stains is, in most cases, the result of a natural process due to the exposure of the stain samples to the environment. Experiences with degraded DNA from casework samples show that every sample may exhibit different properties in this respect, and that it is difficult to systematically assess the performance of routinely used typing systems for the analysis of degraded DNA samples. Using a batch of artificially degraded DNA with an average fragment size of approx. 200 bp a collaborative exercise was carried out among 38 forensic laboratories from 17 European countries. The results were assessed according to correct allele detection, peak heig…
Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans.
1994
A genetic transformation system for the aflatoxin-producing fungus Aspergillus parasiticus using two autonomously replicating plasmids from A. nidulans (ARp1 and pDHG25) is reported. Transformation frequencies using the plasmid pDHG25 were from 5 x 10(2) to 2.5 x 10(4) transformants per 10(6) viable protoplasts and microgram DNA. The stability of the plasmids in the transformants was also studied. This transformation system offers a new opportunity to clone genes related to aflatoxin production using appropriate aflatoxin-defective mutants.
Global data on earthworm abundance, biomass, diversity and corresponding environmental properties
2021
Earthworms are an important soil taxon as ecosystem engineers, providing a variety of crucial ecosystem functions and services. Little is known about their diversity and distribution at large spatial scales, despite the availability of considerable amounts of local-scale data. Earthworm diversity data, obtained from the primary literature or provided directly by authors, were collated with information on site locations, including coordinates, habitat cover, and soil properties. Datasets were required, at a minimum, to include abundance or biomass of earthworms at a site. Where possible, site-level species lists were included, as well as the abundance and biomass of individual species and ec…
Optical remote sensing and the retrieval of terrestrial vegetation bio-geophysical properties – A review
2015
Abstract: Forthcoming superspectral satellite missions dedicated to land monitoring, as well as planned imaging spectrometers, will unleash an unprecedented data stream. The processing requirements for such large data streams involve processing techniques enabling the spatio-temporally explicit quantification of vegetation properties. Typically retrieval must be accurate, robust and fast. Hence, there is a strict requirement to identify next-generation bio-geophysical variable retrieval algorithms which can be molded into an operational processing chain. This paper offers a review of state-of-the-art retrieval methods for quantitative terrestrial bio-geophysical variable extraction using op…
Role of nitric oxide and nitric oxide synthases in experimental models of denervation and reinnervation.
2001
Nitric oxide (NO) is a short-living free molecule synthesized by three different isoforms of nitric oxide synthases (NOS)—neuronal NOS, endothelial NOS, and inducible NOS—associated with neuromuscular transmission, muscle contractility, mitochondrial respiration, and carbohydrate metabolism in skeletal muscle. Neuronal NOS is constitutively expressed at the muscle fiber sarcolemma linked to the dystrophin-glycoprotein complex and concentrated at the neuromuscular endplate. There is increasing evidence that altered expression of neuronal NOS plays a role in muscle fiber damage in neuromuscular diseases such as dystrophinopathies and denervating disorders. Although there have been some previo…
Taenia asiatica: the Most Neglected Human Taenia and the Possibility of Cysticercosis
2013
Not only Taenia solium and Taenia saginata, but also Taenia asiatica infects humans. The last species is not included in the evaluation of the specificity of the immunodiagnostic techniques for taeniasis/cysticercosis. There is currently no specific immunodiagnostic method for T. asiatica available. Therefore, due to the fact that molecular techniques (the only tool to distinguish the 3 Taenia species) are normally not employed in routine diagnostic methods, the 2 questions concerning T. asiatica (its definite geographic distribution and its ability to cause human cysticercosis), remain open, turning T. asiatica into the most neglected agent of human taeniasis-cysticercosis.
Aperiodic Diffract: Study of diffraction gratings
2014
In this work we introduce a virtual laboratory, APERIODIC DIFFRACT, developed in Matlab GUI (Graphical User Interface) as an informatics tool for teaching the diffractive properties of aperiodic gratings. This GUI allows the student to generate aperiodic sequences by iterating and lets to study the spectra for different iterating orders.<br /><br />
Performance of Spectral Fitting Methods for vegetation fluorescence quantification
2010
The Fraunhofer Line Discriminator (FLD) principle has long been considered as the reference method to quantify solar-induced chlorophyll fluorescence (F) from passive remote sensing measurements. Recently, alternative retrieval algorithms based on the spectral fitting of hyperspectral radiance observations, Spectral Fitting Methods (SFMs), have been proposed. The aim of this manuscript is to investigate the performance of such algorithms and to provide relevant information regarding their use. FLD and SFMs were used to estimate F starting from Top Of Canopy (TOC) fluxes at very high spectral resolution (0.12 nm) and sampling interval (0.1 nm), exploiting the O2-B (687.0 nm) and O2-A (760.6 …
Calcification is not the Achilles'heel of cold-water corals in an acidifying ocean
2015
Ocean acidification is thought to be a major threat to coral reefs: laboratory evidence and CO2 seep research has shown adverse effects on many coral species, although a few are resilient. There are concerns that cold-water corals are even more vulnerable as they live in areas where aragonite saturation (Omega ara) is lower than in the tropics and is falling rapidly due to CO2 emissions. Here, we provide laboratory evidence that net (gross calcification minus dissolution) and gross calcification rates of three common cold-water corals, Caryophyllia smithii, Dendrophyllia cornigera, and Desmophyllum dianthus, are not affected by pCO2 levels expected for 2100 (pCO2 1058 µatm, Omega ara 1.29),…