Search results for "ML"
showing 10 items of 1465 documents
Role of interleukin 8 in exosome-mediated crosstalk between chronic myelogenous leukaemia cells and bone marrow stromal cells
2013
In vitro and in vivo effect of CarboxyAmido-Triazole Orotate (CTO) on Imatinib resistant Chronic Myelogenous Leukemia cells
2012
Role of CML exosomes in the crosstalk between chronic myelogenous leukemia and bone-marrow derived exosomes
2012
Crosstalk between chronic myelogenous leukemia (CML) and bone marrow stromal cells: role of interleukin 8 and CML derived- exosomes
2013
Chronic myelogenous leukemia (CML) is a myeloproliferative disorder characterized by the t(9:22) (q34:q11) reciprocal translocation, resulting in the expression of the chimeric Bcr–Abl oncoprotein with constitutive tyrosine kinase activity. Exosomes (Exo) are small vesicles of endosomal origin and of 40-100 nm diameter released by many cell types including cancer cells. Several data indicate that Exo play an important role in cell-to-cell communication and tumor-stroma interaction, thus potentially affecting cancer progression. It is well known that stromal microenvironment contributes to disease progression through the establishment of a bi-directional crosstalk with cancer cells. In the b…
Molecular profiling of imatinib resistant chronic myelogenous leukemia cells
2010
Crosstalk between chronic myelogenous leukemia (CML)and bone marrow stromal cells: role of exosomes in the IL8- dependent signalling mediated by EGFR…
2014
Introduction: CML is a myeloproliferative disorder characterized by Bcr-Abl oncoprotein with a constitutive tyr-kinase activity. Exosomes (exo) shed by cancer cells potentially affect tumor-stroma interaction through the establishment of a bi-directional crosstalk. Interleukin-8 (IL8) is a proinflammatory chemokine that regulate proliferation and survival of cancer cells. We previously demonstrated that CML-derived exo modulate bone marrow microenvironment through the IL8 secretion from stromal cells. EGFR, as well as IL8, regulate cell proliferation and survival; it has been recently demonstrated that EGFR ligands can signal via exosomes shed by cancer cells. We hypothesized that the effec…
Exosomes mediate a paracrine interplay between Chronic Myelogenous Leukemia and stromal cells: a role for interleukin 8
2013
Immunohistochemical Localization of Epithelial, Endothelial and Muscular Cell Differentiation Markers in Human Umbilical Cord
2008
Introduction. Since time [1], our group studies through immunohistochemistry and RT-PCR analysis the expression of many genes and the localization of the corresponding proteins in the human umbilical cord [2], with the aim to create a map of the presence of the different molecules in the different territories of this organ. In this work we investigate the expression and localization of several cell differentiation markers in the different territories of the human umbilical cord. Materials and methods. The presence of E-Cadherin, CD31, CD68, AE1-AE3, Desmin, Actin ML, HHF35 and MIB-1 was studied through immunohistochemistry. Results. The expression of E-Cadherin (epithelial cell marker) has …
Metilazione del DNA in artrite reumatoide
2005
Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…
L’evoluzione delle tecniche di rilevamento negli ultimi quindici anni. Il globo celeste di Mattheus Greuter
2011
Un primo rilevamento del globo celeste di Mattheus Greuter è stato effettuato dallo scrivente nel 1994 con l’impiego di tecniche topografiche e fotogrammetriche tradizionali, prima delle operazioni di restauro che hanno restituito al mappamondo il suo aspetto originario. Nel mese di giugno di quest’anno è stato eseguito un secondo rilevamento con l’impiego delle tecniche più recenti (laser scanning, fotogrammetria digitale). Il confronto fra i due rilevamenti fornisce interessanti indicazioni per valutare l’evoluzione delle tecniche in questi quindici anni.