Search results for "PAM"

Extracellular site of action of phenylalkylamines on L-type calcium current in rat ventricular myocytes.

1995

The effects of the phenylalkylamines verapamil, gallopamil, and devapamil on L-type calcium currents (ICa) were studied in ventricular myocytes from rat hearts using the whole-cell patch-clamp technique. In particular, the question was addressed, whether the pharmacological binding sites for these drugs were located at the inner and/or at the outer surface of the cell membrane. Therefore, tertiary verapamil, gallopamil, and devapamil and their corresponding quaternary derivatives were applied either from the outside or the inside of the cell membrane. Extracellular application of verapamil, gallopamil and devapamil (each at 3 microM) reduced ICa to 16.1 +/- 8.6%, 11 +/- 8.9%, and 9.3 +/- 6%…

Análisis de la sostenibilidad del sector exterior en la OCDE con técnicas de multicointegración

2022

Este artículo analiza la sostenibilidad externa de un grupo de 23 países de la OCDE durante el periodo 1970-2020. La estrategia empírica adoptada muestra la importancia del desequilibrio acumulado como desencadenante del ajuste externo, en línea con la propuesta de Gourinchas y Rey (2007). Para ello, contrastamos la sostenibilidad externa a través de la relación acumulado-flujo entre las variables que representan las relaciones externas de una economía abierta utilizando varios tipos de contrastes de cointegración y multicointegración. Los resultados obtenidos apuntan hacia la sostenibilidad en sentido débil en el análisis de los flujos, mientras que desde el punto de vista del enfoque acum…

On-line photoreaction and fluorimetric determination of diazepam.

1993

Prenatal diazepam exposure alters neurosteroid modulation of NMDA receptors regulating noradrenaline release from rat hippocampus

2002

Ģeometrijas mācīšanas optimizācija pamatskolā

2005

A Diet for Dopaminergic Neurons?

2009

Parkinson's disease (PD) is the second most common neurodegenerative disease, which unfortunately is still fatal. Since the discovery of dopamine (DA) neuronal cell loss within the substantia nigra in PD, the past decades have seen the understanding of the pathophysiological mechanisms underlying the degenerative process advance at a very impressive rate. Nevertheless, there is at present no cure for PD. Although there are no proven therapies for prevention, a large body of evidence from animal studies has highlighted the paramount role of dietary factors in counteracting DA degeneration. Consistently, associations between the risk of developing PD and the intake of nutrients, individual fo…

Cytotoxicity of cardiotonic steroids in sensitive and multidrug-resistant leukemia cells and the link with Na(+)/K(+)-ATPase.

2015

Cardiotonic steroids have long been in clinical use for treatment of heart failure and are now emerging as promising agents in various diseases, especially cancer. Their main target is Na(+)/K(+)-ATPase, a membrane protein involved in cellular ion homeostasis. Na(+)/K(+)-ATPase has been implicated in cancer biology by affecting several cellular events and signaling pathways in both sensitive and drug-resistant cancer cells. Hence, we investigated the cytotoxic activities of 66 cardiotonic steroids and cardiotonic steroid derivatives in sensitive CCRF-CEM and multidrug-resistant CEM/ADR5000 leukemia cells. Data were then subjected to quantitative structure-activity relationship analysis (QSA…

Induction of Human P-Glycoprotein in Caco-2 cells: Development of a Highly Sensitive Assay System for P-Glycoprotein-Mediated Drug Transport

2006

The aim of this work is to develop a highly sensitive assay system for P-gp-mediated transport by using two methods, induction of P-gp and short-term culture of Caco-2 cells. To induce P-gp in Caco-2 cells, cells were cultured in vinblastine-containing medium. The mRNA level of P-gp was approximately 7-fold higher in Caco-2 cells cultured with vinblastine (P-gp-induced Caco-2 cells) than in control cells. Western blot analysis showed a significant increase in P-gp expression. After cell differentiation, the mRNA level of P-gp was downregulated, however, P-gp-induced Caco-2 cells still possessed a 5.6-fold higher mRNA level of P-gp compared to control cells. Polarized transport of substrate …

Oxidation of dobutamine and dopamine by horseradish peroxidase

2022

Dobutamine and dopamine have been previously shown to interfere with enzymatic diagnostic tests and different mechanisms responsible for this effect have been postulated. We have studied the oxidation by horseradish peroxidase (HRP) of dopamine, dobutamine, and its analog with the phenol group blocked by methylation. Oxidation of dobutamine was much faster than dopamine, as reported before, whereas the methylated analog of dobutamine was oxidized at intermediate rate. This demonstrates that the phenol group in dobutamine is oxidized preferentially by HRP and acts as an electron-transfer mediator in oxidation of the catechol group. Different oxidation rates of catechol groups in dopamine and…

Preiļu novada Salas pamatskolas vēsture dokumentos un mutvārdu vēstures stāstos

2019

Bakalaura darba “Preiļu novada Salas pamatskolas vēsture dokumentos un mutvārdu vēstures stāstos” mērķis ir izpētīt vēl līdz šim neizpētītu vēsturi par Preiļu novada Salas pamatskolu, veikt vēstures dokumentu izpēti un ierakstīt mutvārdu vēstures liecības, rezultātā iegūt vērtīgus materiālus un saglabāt tos. Pētījuma teorētisko pamatu veido dokumentu analīzes, mutvārdu vēstures un interviju apskats, kā arī sociālā kapitāla teorijas apskats. Pētījumā izmantotas tādas pētniecības metodes kā dokumentu analīze un nestrukturētā intervija. Pētījuma rezultātā tika iegūta informācija par Preiļu novada Salas pamatskolas vēsturi – tās pirmsākumiem, attīstību, cilvēkiem, notikumiem līdz pat mūsdienām.…