Search results for "Precipitation"

showing 10 items of 826 documents

Intelligent system for electrical heating control based on optical precipitation sensor

2018

Abstract The paper presents testing results of the SCADA system IntelHeat introduced at the Latvian Railways for automated heating control of railway points during winters with the purpose of electricity saving by optimized real-time assignment of heating levels. The system is based on innovative optical sensors of precipitation detecting rain and snow particles using diffuse and specular reflection channels. The results of trial exploitation demonstrated the ability of the IntelHeat system to manage points heating flexibly in accordance with changes of weather conditions and correspondence with archived meteorological data.

SCADAbusiness.industry020209 energy0202 electrical engineering electronic engineering information engineeringEnvironmental science02 engineering and technologySpecular reflectionElectricityPrecipitationbusinessRain and snow mixedRemote sensingEnergy Procedia
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Functional Connection Between the Clb5 Cyclin, the Protein Kinase C Pathway and the Swi4 Transcription Factor in Saccharomyces cerevisiae

2005

Abstract The rsf12 mutation was isolated in a synthetic lethal screen for genes functionally interacting with Swi4. RSF12 is CLB5. The clb5 swi4 mutant cells arrest at G2/M due to the activation of the DNA-damage checkpoint. Defects in DNA integrity was confirmed by the increased rates of chromosome loss and mitotic recombination. Other results suggest the presence of additional defects related to morphogenesis. Interestingly, genes of the PKC pathway rescue the growth defect of clb5 swi4, and pkc1 and slt2 mutations are synthetic lethal with clb5, pointing to a connection between Clb5, the PKC pathway, and Swi4. Different observations suggest that like Clb5, the PKC pathway and Swi4 are in…

Saccharomyces cerevisiae ProteinsMitotic crossoverBlotting WesternMutantSaccharomyces cerevisiaeSaccharomyces cerevisiaeInvestigationsCyclin BBiologymedicine.disease_causeGeneticsmedicineHydroxyureaImmunoprecipitationDNA FungalFluorescent Antibody Technique IndirectTranscription factorProtein Kinase CProtein kinase CCyclinRecombination GeneticGeneticsMutationKinaseCell CyclefungiFlow Cytometrybiology.organism_classificationMolecular biologyCell biologyDNA-Binding ProteinsMutationChromosomes FungalTranscription FactorsGenetics
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Functional distinction between Cln1p and Cln2p cyclins in the control of the Saccharomyces cerevisiae mitotic cycle.

2004

Abstract Cln1p and Cln2p are considered as equivalent cyclins on the basis of sequence homology, regulation, and functional studies. Here we describe a functional distinction between the Cln1p and Cln2p cyclins in the control of the G1/S transition. Inactivation of CLN2, but not of CLN1, leads to a larger-than-normal cell size, whereas overexpression of CLN2, but not of CLN1, results in smaller-than-normal cells. Furthermore, mild ectopic expression of CLN2, but not of CLN1, suppresses the lethality of swi4swi6 and cdc28 mutant strains. In the absence of Cln1p, the kinetics of budding, initiation of DNA replication, and activation of the Start-transcription program are not affected; by cont…

Saccharomyces cerevisiae ProteinsMutantSaccharomyces cerevisiaeBlotting WesternMitosisSaccharomyces cerevisiaeBiologyInvestigationsmedicine.disease_causeS PhaseCyclinsGeneticsmedicineImmunoprecipitationFluorescent Antibody Technique IndirectMitosisCyclinCell SizeGeneticsCyclin-dependent kinase 1MutationDNA replicationbiology.organism_classificationBlotting NorthernBridged Bicyclo Compounds HeterocyclicFlow CytometryMolecular biologyThiazolesMutationThiazolidinesEctopic expressionGenetics
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Trx2p-dependent Regulation of Saccharomyces cerevisiae Oxidative Stress Response by the Skn7p Transcription Factor under Respiring Conditions

2013

The whole genome analysis has demonstrated that wine yeasts undergo changes in promoter regions and variations in gene copy number, which make them different to lab strains and help them better adapt to stressful conditions during winemaking, where oxidative stress plays a critical role. Since cytoplasmic thioredoxin II, a small protein with thiol-disulphide oxidoreductase activity, has been seen to perform important functions under biomass propagation conditions of wine yeasts, we studied the involvement of Trx2p in the molecular regulation of the oxidative stress transcriptional response on these strains. In this study, we analyzed the expression levels of several oxidative stress-related…

Saccharomyces cerevisiae ProteinsSaccharomyces cerevisiaeBlotting WesternMolecular Sequence Datalcsh:MedicineWineOxidative phosphorylationSaccharomyces cerevisiaemedicine.disease_causePolymerase Chain ReactionThioredoxinsGene Expression Regulation FungalGene expressionmedicineImmunoprecipitationPhosphorylationlcsh:ScienceTranscription factorHeat-shock responseDNA PrimersRegulation of gene expressionMultidisciplinarybiologyBase Sequencelcsh:RPromoterbiology.organism_classificationCatalasebeta-GalactosidaseYeastGene regulationDNA-Binding ProteinsOxidative StressBiochemistryOxidative stresslcsh:QGene expressionThioredoxinTranscription factorOxidative stressGene DeletionResearch ArticlePlasmidsTranscription FactorsPLoS ONE
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A short-range gradient of histone H3 acetylation and Tup1p redistribution at the promoter of the Saccharomyces cerevisiae SUC2 gene.

2003

Chromatin immunoprecipitation assays are used to map H3 and H4 acetylation over the promoter nucleosomes and the coding region of the Saccharomyces cerevisiae SUC2 gene, under repressed and derepressed conditions, using wild type and mutant strains. In wild type cells, a high level of H3 acetylation at the distal end of the promoter drops sharply toward the proximal nucleosome that covers the TATA box, a gradient that become even steeper on derepression. In contrast, substantial H4 acetylation shows no such gradient and extends into the coding region. Overall levels of both H3 and H4 acetylation rise on derepression. Mutation of GCN5 or SNF2 lead to substantially reduced SUC2 expression; in…

Saccharomyces cerevisiae ProteinsTATA boxMutantGene ExpressionSaccharomyces cerevisiaeBiologyBiochemistryPolymerase Chain ReactionHistonesNucleosomeRNA MessengerHistone H3 acetylationDNA FungalPromoter Regions GeneticMolecular BiologyDerepressionHistone AcetyltransferasesAdenosine Triphosphatasesbeta-FructofuranosidaseWild typeChromosome MappingNuclear ProteinsCell BiologyMolecular biologyDNA-Binding ProteinsRepressor ProteinsAcetylationMutagenesisChromatin immunoprecipitationProtein KinasesTranscription FactorsThe Journal of biological chemistry
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Yeast HAT1 and HAT2 deletions have different life-span and transcriptome phenotypes

2005

AbstractHAT-B is a yeast histone acetyltransferase composed of Hat1, Hat2 and Hif1 proteins. We demonstrate that a hat2 mutant or a hat1hat2 double mutant, but not a hat1 mutant, have an extended life-span. Transcriptome analysis shows that the single hat mutants are not very different from wild type. However, the comparison of the hat1 and hat2 transcriptomes shows that they are different. The hat1hat2 double mutant shows a transcriptional phenotype similar to that of the hat1 mutant but strongly enhanced. These results indicate that Hat2p could have additional functions in the cell to those of Hat1p.

Saccharomyces cerevisiae ProteinsTranscription GeneticHAT-BMutantBiophysicsSaccharomyces cerevisiaeBiochemistryTranscriptomeDNA-chipAcetyltransferasesStructural BiologyHat2Life-spanGeneticsImmunoprecipitationSirtuinsMolecular BiologyHistone AcetyltransferasesGeneticsbiologyWild typeCell BiologyHistone acetyltransferaseTelomereHat1PhenotypeYeastPhenotypebiology.proteinHistone deacetylaseHAT1Gene DeletionFEBS Letters
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2018

Abstract. Mineral dust is an important component of the climate system, affecting the radiation balance, cloud properties, biogeochemical cycles, regional circulation and precipitation, as well as having negative effects on aviation, solar energy generation and human health. Dust size and composition has an impact on all these processes. However, changes in dust size distribution and composition during transport, particularly for coarse particles, are poorly understood and poorly represented in climate models. Here we present new in situ airborne observations of dust in the Saharan Air Layer (SAL) and the marine boundary layer (MBL) at the beginning of its transatlantic transport pathway, f…

Saharan Air LayerEarth's energy budgetAtmospheric Sciencefood.ingredient010504 meteorology & atmospheric sciencesSea saltMineral dustSedimentation010502 geochemistry & geophysicsAtmospheric sciences01 natural sciencesAerosolfoodAltitude13. Climate actionEnvironmental sciencePrecipitation0105 earth and related environmental sciencesAtmospheric Chemistry and Physics
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Using Satellite precipitation data to support local climatology and weather monitoring. Application to Sicilia.

2012

SatelliteSettore ICAR/02 - Costruzioni Idrauliche E Marittime E Idrologiaprecipitation
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Isolation of the silicatein-α interactor silintaphin-2 by a novel solid-phase pull-down assay.

2011

The skeleton of siliceous sponges consists of amorphous biogenous silica (biosilica). Biosilica formation is driven enzymatically by means of silicatein(s). During this unique process of enzymatic polycondensation, skeletal elements (spicules) that enfold a central proteinaceous structure (axial filament), mainly comprising silicatein, are formed. However, only the concerted action of silicatein and other proteins can explain the genetically controlled diversity of spicular morphotypes, from simple rods with pointed ends to intricate structures with up to six rays. With the scaffold protein silintaphin-1, a first silicatein interactor that facilitates the formation of the axial filament and…

Scaffold proteinSpiculeImmunoprecipitationMolecular Sequence DataNanotechnologyBiologyFlagellumBiochemistry03 medical and health sciencesSponge spiculePhase (matter)Two-Hybrid System TechniquesProtein Interaction MappingAnimalsInteractorAmino Acid Sequence030304 developmental biology0303 health sciences030302 biochemistry & molecular biologySilicon DioxideCathepsinsYeastProtein TransportSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationBiophysicsAutoradiographyCalciumSuberitesProtein BindingBiochemistry
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Superparameterised cloud effects in the EMAC general circulation model (v2.50) – influences of model configuration

2020

A new module has been implemented in the fifth generation of the ECMWF/Hamburg (ECHAM5)/Modular Earth Submodel System (MESSy) Atmospheric Chemistry (EMAC) model that simulates cloud-related processes on a much smaller grid. This so-called superparameterisation acts as a replacement for the convection parameterisation and large-scale cloud scheme. The concept of embedding a cloud-resolving model (CRM) inside of each grid box of a general circulation model leads to an explicit representation of cloud dynamics. The new model component is evaluated against observations and the conventional usage of EMAC using a convection parameterisation. In particular, effects of applying different configurat…

Scale (ratio)Meteorologybusiness.industryCloud coverlcsh:QE1-996.5Cloud computingSensible heatGridlcsh:GeologyDiurnal cycleEnvironmental scienceClimate statePrecipitationbusinessGeoscientific Model Development
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