Search results for "RNAS"
showing 10 items of 710 documents
Interplay Between MicroRNAs and Oxidative Stress in Ovarian Conditions with a Focus on Ovarian Cancer and Endometriosis
2019
Ovarian cancer and endometriosis are two distinct gynaecological conditions that share many biological aspects incuding proliferation, invasion of surrounding tissue, inflammation, inhibition of apoptosis, deregulation of angiogenesis and the ability to spread at a distance. miRNAs are small non-coding RNAs (19–22 nt) that act as post-transcriptional modulators of gene expression and are involved in several of the aforementioned processes. In addition, a growing body of evidence supports the contribution of oxidative stress (OS) to these gynaecological diseases: increased peritoneal OS due to the decomposition of retrograde menstruation blood facilitates both endometriotic lesion developmen…
Characterization of a profile of epigenetic alterations involved in the aetiopathogenesis of endometriosis. Validation of molecular biomarkers for di…
2018
Introduction: Endometriosis is an oestrogen-dependent inflammatory disorder defined by the presence of endometrial-like tissue in ectopic locations, which limits the quality of life of affected women. This pathology affects 10% of reproductive-age women from all ethnic and social groups, although the prevalence in those patients experiencing pain, infertility or both is as high as 35%-50%, being the estimated prevalence of this condition around 176 million worldwide. Endometriosis is associated with an average diagnostic delay of 7 years, what could be partially explained by the lack of non-invasive biomarkers for diagnose, since the gold standard method is laparoscopy followed by histologi…
Identificación de los mecanismos epigenéticos relacionados con los fenómenos de inmunosupresión en sepsis y su uso como biomarcadores de pronóstico
2023
La sepsis es un síndrome complejo que provoca una respuesta inmunitaria exacerbada como resultado a una infección, lo que puede causar disfunción orgánica y, en última instancia, la muerte. La sepsis puede avanzar a shock séptico (SS), caracterizado por una disfunción orgánica aguda que puede provocar una insuficiencia circulatoria y metabólica grave, aumentando el riesgo de mortalidad. Los pacientes con sepsis o SS ingresados durante al menos 7 días en la Unidad de Cuidados Intensivos (UCI) que presentan disfunción orgánica y necesidad de ventilación mecánica se clasifican como enfermos críticos crónicos (ECC), los cuales, si están ingresados al menos 14 días y cumplen una serie de caracte…
I vari modi del DNA di “parlare” ai viventi fra ciò che già è noto e nuove scoperte
2018
A sessant’anni da quando Francis Crick enunciò il “dogma centrale della biologia molecolare” molte scoperte e nuove frontiere si sono avvicendate sulla questione “funzionale” di un genoma, cioè di come un DNA “parla” ai viventi. Oggi è abbastanza noto come un genoma “è”, ed occuparsi solo di questa questione strutturale ormai è sicuramente molto riduttivo, almeno sicuramente per quello umano. È invece il goal standard di questi anni scoprire come e quando un genoma si esprime, come funzio-nalmente traduce il suo messaggio rendendo questo visibile nei fe-notipi dei viventi, a prescindere dalla sua sequenza primaria di basi azotate. Ne consegue che il codice genetico, ancora valido e quasi un…
MiR-33a Controls hMSCS Osteoblast Commitment Modulating the Yap/Taz Expression Through EGFR Signaling Regulation
2019
Mesenchymal stromal cells (hMSCs) display a pleiotropic function in bone regeneration. The signaling involved in osteoblast commitment is still not completely understood, and that determines the failure of current therapies being used. In our recent studies, we identified two miRNAs as regulators of hMSCs osteoblast differentiation driving hypoxia signaling and cytoskeletal reorganization. Other signalings involved in this process are epithelial to mesenchymal transition (EMT) and epidermal growth factor receptor (EGFR) signalings through the regulation of Yes-associated protein (YAP)/PDZ-binding motif (TAZ) expression. In the current study, we investigated the role of miR-33a family as a (…
Estradiol deficiency and skeletal muscle apoptosis: Possible contribution of microRNAs.
2020
Background Menopause leads to estradiol (E2) deficiency that is associated with decreases in muscle mass and strength. Here we studied the effect of E2 deficiency on miR-signaling that targets apoptotic pathways. Methods C57BL6 mice were divided into control (normal estrous cycle, n = 8), OVX (E2 deficiency, n = 7) and OVX + E2 groups (E2-pellet, n = 4). Six weeks following the OVX surgery, mice were sacrificed and RNA isolated from gastrocnemius muscles. miR-profiles were studied with Next-generation sequencing (NGS) and candidate miRs verified using qPCR. The target proteins of the miRs were found using in silico analysis and measured at mRNA (qPCR) and protein levels (Western blot). Resu…
Transcriptional Rewiring, Adaptation, and the Role of Gene Duplication in the Metabolism of Ethanol of Saccharomyces cerevisiae
2020
Ethanol is the main by-product of yeast sugar fermentation that affects microbial growth parameters, being considered a dual molecule, a nutrient and a stressor. Previous works demonstrated that the budding yeast arose after an ancient hybridization process resulted in a tier of duplicated genes within its genome, many of them with implications in this ethanol “produce-accumulate-consume” strategy. The evolutionary link between ethanol production, consumption, and tolerance versus ploidy and stability of the hybrids is an ongoing debatable issue. The implication of ancestral duplicates in this metabolic rewiring, and how these duplicates differ transcriptionally, remains unsolved. Here, we …
Efecto del entrenamiento de fuerza explosiva sobre las características básicas y específicas de la fuerza en jugadoras de voleibol
2012
La investigación sobre el efecto del entrenamiento de fuerza explosiva sobre las características básicas y específicas de la fuerza explosiva, se ha realizado con 40 jugadoras de voleibol divididas en tres grupos: Un grupo específico formado por 12 jugadoras de elite que ha sido sometido al proceso de entrenamiento específico con objetivo de desarrollar la fuerza explosiva de los extensores de espalda, los extensores de piernas y los extensores de tobillos. Un segundo grupo clásico formado por 11 jugadoras de voleibol que han realizado entrenamiento clásico sin impacto en la fuerza explosiva. Y un tercer grupo control formado por 20 mujeres sanas, no entrenadas y con buena condición física.…
La lettera di Adriano ad Alicarnasso e la cultura storico-antiquaria in età adrianea. Riflessioni sull’iscrizione AE 2012, 1550
2016
Nel 2012 è stato pubblicato il frammento epigrafico di una lettera dell'imperatore Adriano alla città di Alicarnasso in Caria (AE 2012, 1550). Gli autori presentano alcune riflessioni sulla cronologia, sui contenuti superstiti e sulla cornice culturale di questo interessante, seppur lacunoso, documento. L'analisi della titolatura imperiale adrianea permette di affermare che la lettera è stata scritta tra il 128 e il 135, come risposta a una petizione di Alicarnasso. Si ipotizza che la città fosse stata danneggiata nel 128 da un terremoto, che avrebbe colpito anche Rodi, Astypalaia e altre località costiere della provincia Asia. Adriano avrebbe concesso finanziamenti pubblici per la ricostru…
Elpceļu arodslimību riska faktori internās māsas praksē
2015
Bakalaura darbs „Elpceļu arodslimību riska faktori internās māsas praksē”. Rīga, Latvijas Universitāte medicīnas fakultāte, profesionālā augstākās izglītības bakalaura studiju programma „Māszinības”, zinātniskais darba vadītājs mg. paed. Inese Paudere. Darbs sastāv no 46 lpp., 11 att., 3 tab., 49 bibliogrāfiski nosaukumi latviešu, krievu, angļu valodā. Pētījumā tika noskaidroti elpceļu arodslimību riska faktori internās māsas praksē. Pētījuma hipotēze: elpceļu arodslimību riska faktori saistīti ar darba apstākļiem. Ievada daļā darba autore iepazīstina ar pētniecības darba aktualitāti ar elpceļu arodslimību riska faktoriem internās māsas praksē. Teorētiskajā daļā ir aplūkotas elpceļu arodsli…