Search results for "Tos"
showing 10 items of 12217 documents
Delivery of proteins into living cells by reversible membrane permeabilization with streptolysin-O
2001
The pore-forming toxin streptolysin O (SLO) can be used to reversibly permeabilize adherent and nonadherent cells, allowing delivery of molecules with up to 100 kDa mass to the cytosol. Using FITC-labeled albumin, 10 5 –10 6 molecules were estimated to be entrapped per cell. Repair of toxin lesions depended on Ca 2+ -calmodulin and on intact microtubules, but was not sensitive to actin disruption or to inhibition of protein synthesis. Resealed cells were viable for days and retained the capacity to endocytose and to proliferate. The active domains of large clostridial toxins were introduced into three different cell lines. The domains were derived from Clostridium difficile B-toxin and Clo…
Mtlinteracts with members of Egfr signaling and cell adhesion genes in the Drosophila eye
2011
Mtl is a member of the Rho family of small GTPases in Drosophila. It was shown that Mtl is involved in planar cell polarity (PCP) establishment, together with other members of the same family like Cdc42, Rac1, Rac2 and RhoA. However, while Rac1, Rac2 and RhoA function downstream of Dsh in Fz/PCP signaling and upstream of a JNK cassette, Mtl and Cdc42 do not. To determine the functional context of Mtl during PCP establishment in the Drosophila eye, we performed a loss-of-function screen to search for dominant modifiers of a sev>Mtl rough eye phenotype. In addition, genetic interaction assays with candidate genes were also carried out. Our results show that Mtl interacts genetically with memb…
A novel microtubule de-stabilizing complementarity-determining region C36L1 peptide displays antitumor activity against melanoma in vitro and in vivo
2015
AbstractShort peptide sequences from complementarity-determining regions (CDRs) of different immunoglobulins may exert anti-infective, immunomodulatory and antitumor activities regardless of the specificity of the original monoclonal antibody (mAb). In this sense, they resemble early molecules of innate immunity. C36L1 was identified as a bioactive light-chain CDR1 peptide by screening 19 conserved CDR sequences targeting murine B16F10-Nex2 melanoma. The 17-amino acid peptide is readily taken up by melanoma cells and acts on microtubules causing depolymerization, stress of the endoplasmic reticulum and intrinsic apoptosis. At low concentrations, C36L1 inhibited migration, invasion and proli…
Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in …
2010
The organization and dynamics of microtubules (MTs) and the actin cytoskeleton are critical for the correct development and functions of neurons, including intracellular traffic and signaling. In vitro ethanol exposure impairs endocytosis, exocytosis, and nucleocytoplasmic traffic in astrocytes and alters endocytosis in cultured neurons. In astrocytes, these effects relate to changes in the organization and/or function of MTs and the actin cytoskeleton. To evaluate this possibility in hippocampal cultured neurons, we analyzed if chronic ethanol exposure affects the levels, assembly, and cellular organization of both cytoskeleton elements and the possible underlying mechanisms of these effec…
I fattori terapeutici nella psicoterapia di gruppo per disturbi da uso di sostanze. Considerazioni cliniche a partire da un contributo empirico
2013
L'articolo propone alcune riflessioni sui gruppi psicoterapeutici ad orientamento psicodinamico con pazienti tossicodipendenti a partire da una ricerca focalizzata sull'analisi del processo e sui fattori terapeutici. Viene anche presentata l'analisi degli elementi processuali che la letteratura ritiene rilevanti nel favorire i processi di cambiamento psicologico
Enfermería y medicamentos peligrosos: una aproximación a las condiciones y consecuencias de su manipulación
2023
La salud laboral es un aspecto determinante para el sector sanitario. Existe una amplia variedad de riesgos y las normas legales exigen que sean evitados o reducidos al máximo. La manipulación de medicamentos peligrosos (MP) constituye un riesgo específico, significativo y poco conocido que afecta al desempeño de las enfermeras en todas las áreas asistenciales. Desde hace décadas se ha prestado atención a los riesgos de la manipulación de medicamentos antineoplásicos, siendo mucho más reciente la aceptación de que otros muchos medicamentos, que pueden utilizarse en diversas áreas asistenciales, pueden comportar riesgos para la salud comparables a los de los quimioterápicos. Los MP son un gr…
Intestinal protozoa in HIV-infected patients: effect of rifaximin in Cryptosporidium parvum and Blastocystis hominis infections
1999
In HIV-1 infected patients severe enteritis and chronic diarrhea are often documented as a consequence of multiple opportunistic infections. We analyzed 48 HIV-1 positive patients for the presence of intestinal pathogenic protozoa. Patients with CD4 > or = 200/mm3 showed a higher prevalence of a single pathogenic protozoa than patients with CD4 or = 200/mm3, who presented enteric and systemic symptoms due to Criptosporidium or Blastocystis associated with enteropathogenic bacteria
Rinnakkaisopetussuunnitelmasta yhteisöllisyyttä, osaamisen tunnistamista ja käytännön kokemusta yliopisto-opintojen ohessa
2022
Tässä artikkelissa kuvataan suomalaiselle koulutusjärjestelmälle vieraampaa toimintamuotoa, rinnakkaisopintosuunnitelmatoimintaa (extra-curricular activities). Toimintaa pilotoitiin Jyväskylän yliopistossa, jossa sen toteutusta seurattiin tutkimuksen avulla viisi vuotta. Kansainvälisten rinnakkaisopintosuunnitelmamallien mukaisesti suomalainen kokeilu perustui vapaaehtoistyön tekemiseen, mutta siihen kytkettiin mukaan ryhmäohjaus, joka tuki opiskelijan työskentelyä vapaaehtoistyöpaikassa ja auttoi opiskelijaa tunnistamaan kokeilun aikana kehittyvää osaamistaan. Pitkittäistutkimuksen aineisto kerättiin sekä haastatteluina että kyselylomakkeilla. Tulokset osoittavat, että rinnakkaisopintosuun…
Risk map of transmission of urogenital schistosomiasis by Bulinus truncatus (Audouin, 1827) (Mollusca Gastropoda, Bulinidae) in Spain and Portugal
2019
Mapa del riesgo de contraer schistosomiasis urogenital provocada por Bulinus truncatus (Audouin, 1827) (Mollusca Gastropoda, Bulinidae) en España y Portugal Se da a conocer el mapa de la distribución geográfica de Bulinus truncatus en España y Portugal en el que se recopilan las localidades históricas y actuales, que coincide con el mapa del riesgo de contraer schistosomiasis urogenital provocada por este caracol de agua dulce. Se revisan las muestras de esta especie depositadas en el Museu de Ciències Naturals de Barcelona y en el Museo de Ciencias Naturales de Madrid, así como datos propios, incluidas algunas aportaciones inéditas. Este mapa permitirá conocer el área óptima de esta especi…
Valutazione delle lesioni focali del tendine del sovraspinato con elastosonografia: confronto con ecografia B-mode e risonanza magnetica: esperienza …
2012
Obiettivo. Scopo dello studio era valutare l’elasticità del tendine del sovraspinato nella spalla sintomatica di pazienti con sospetto clinico di rottura unilaterale della cuffia dei rotatori, e comparare i reperti riscontrati con quelli dell’ecografia convenzionale (ultrasound, US) e della risonanza magnetica (RM). Metodi. Nel periodo compreso tra gennaio e dicembre 2009 sono state esaminate da un radiologo entrambe le spalle di 58 pazienti (età media: 46 anni; range 32-58 anni) sia con modulo elastosonografico in real-time che in B-mode tramite uno stesso ecografo (EUB - Hitachi 7500), usando una sonda lineare a elevata frequenza (13 MHz). L’elasticità delle fibre tendinee del sovraspinat…