Search results for "Vegetal Biology"
showing 10 items of 1601 documents
Technical improvements for analysis of recalcitrant proteins by LC-MS : the mycorrhiza responsive membrane proteome as a case study
2012
Arbuscular mycorrhizas (AM) are widespread symbiotic associations between plant roots and AM fungi. Deep membrane alterations are the foremost morphological changes occurring in the host plant in response to AM symbiosis. Two-dimensional gel electrophoresis (2-DE) is the workhorse method in AM proteomics. Membrane proteins are under-represented in 2-DE because of their hydrophobicity, low abundance, and precipitation at their isoelectric point, thereby few are the identified membrane proteins involved in sustaining the AM symbiosis. Membrane proteomics is still challenging due to 2-DE related shortcomings, however latest trends and advancements in mass spectrometry (MS)-based quantitative p…
Création et rôle de l'Observatoire de l'ambroisie
2012
L’envahissement du territoire français par l’ambroisie à feuilles d’armoise constitue à la fois une préoccupation de santé publique et un problème agricole important. Le ministère chargé de la Santé et l’Institut National de la Recherche Agronomique ont mis en place en juin 2011 l’« Observatoire de l’ambroisie » qui a pour mission principale la coordination des actions de lutte contre cette plante allergisante. Avec pour objectif de devenir un centre de ressources de références en matière d’ambroisie, l’observatoire contribuera à valoriser la connaissance sur la plante, notamment ses effets sur la santé, sur les actions durables de prévention, ainsi que sur les données scientifiques et les …
Cryptogein signaling in tobacco: in search for nitric oxide targets
2013
Nitric oxide (NO) triggers various physiological responses in plants. Notably, NO is recognized to account for the response to biotic stresses. We previously reported that NO is produced in tobacco cells exposed to cryptogein, a 10 kDa elicitor secreted by the oomycete Phytophthora cryptogea. To decipher the role of NO, we identified and characterized S-nitrosylated proteins in tobacco cell suspensions elicited by cryptogein. Several candidates were identified including the chaperone-like AAA+ATPase CDC48 and a calmodulin isoform (CaM). Interestingly, the Cys residue undergoing S-nitrosylation in CaM is located in the first Ca2+ binding EF hand and is not or poorly conserved in other organi…
Grass Pea
2011
Grass pea (Lathyrus. sativus L.) is an annual legume crop with high protein content and remarkable resistance to extreme environmental conditions, including fl ooding, drought, salinity and low soil fertility, and a signifi cant degree of resistance to biotic stress agents. It is rightly considered one of the most promising sources of calories and protein for the vast and expanding populations of drought-prone and marginal areas of Asia and Africa and as an interesting alternative for cropping systems diversifi cation in marginal lands in Europe, Australia and America. It is a dual purpose crop with great agronomic potential as a grain and forage legume. Nevertheless, as a result of the lit…
Next-Generation Sequencing (NGS) application to the detection of mutations endowing herbicide resistance
2014
The Next Generation Sequencing (NGS) technologies offer tremendous possibilities for accurate detection of mutations endowing resistance. Yet, their use for this purpose has not emerged in crop protection. We aim at promoting NGS use for herbicide resistance diagnosis. In an exemplary study, we describe a simple procedure for this purpose, useable by virtually any scientist and implementing freely accessible programs for the analysis of NGS data. Three PCR amplicons encompassing seven codons of the ALS gene that are crucial for herbicide resistance were sequenced using non-quantified pools of crude DNA extracts from 40 plants in each of 28 field populations of two species of barnyard-grass:…
European Weed Research Society (EWRS) workshop "Herbicide-Resistance in Europe: challenges, opportunities and threats"
2014
To resistance mechanisms and beyond: an evolutionary approach of herbicide resistance
2014
The diversity of herbicide resistance mechanisms observed today in weed populations is the result of a very rapid evolution in response to the selection pressure exerted by herbicide applications. Herbicide resistance is a dynamic process: weed populations are undoubtedly still continuing to evolve, so that the long-term outcome of herbicide selection is currently unpredictable. The vast majority of the studies addressing herbicide resistance published to date are focussed on the identification of mechanisms endowing resistance, with the applied aim to develop resistance diagnosis methods. Understanding resistance evolution is key to set up proactive weed management strategies that should i…
The success of Agronomy for Sustainable Development: from IF 0.566 rank 29/53 to IF 2.9 rank 4/75
2012
Scientific journals are facing an increasing competition directly impacting publishers and editorial boards. In addition, access to scientific information and reading patterns have deeply changed. From 2003 the journal Agronomy for Sustainable Development (ASD) has adapted its editorial policy to this highly competitive and moving environment in order to stand out and improve its impact (1). The scope, language and title of the journal were modified. A preselection step was introduced, several calls for reviews were made and spin-off books were published (2). Particular attention was given to abstracts, titles, colour figures and photos. A sister book series named Sustainable Agriculture Re…
La prise en compte de la stratégie agricole améliore la compréhension de l’effet des pratiques sur les communautés adventices
2019
National audience; Les agriculteurs présents dans une même situation de production peuvent différer dans leurs objectifs et donc leurs pratiques. Afin de tester l’effet d’une pratique particulière (ex. désherbage chimique) sur la communauté adventice, il est donc nécessaire de tenir compte de l’ensemble des autres pratiques, formant une stratégie agricole. Nous avons ainsi développé 14 indicateurs décrivant les pratiques agricoles enquêtées sur la zone de Fénay de 2004 à 2016 auprès des 23 agriculteurs de la zone. L’originalité des indicateurs est qu’ils situent chaque parcelle par rapport aux autres parcelles ayant mis en oeuvre la même culture la même année sur la zone. Ceci nous a permis…
Maladie du huanglongbing
2019
Maladie du huanglongbing. Analyse du risque phytosanitaire pour l’Union européenne