Search results for "Virus Disease"
showing 10 items of 1907 documents
Coxsackievirus B3 VLPs purified by ion exchange chromatography elicit strong immune responses in mice
2014
Coxsackievirus B3 (CVB3) is an important cause of acute and chronic viral myocarditis, and dilated cardiomyopathy (DCM). Although vaccination against CVB3 could significantly reduce the incidence of serious or fatal viral myocarditis and various other diseases associated with CVB3 infection, there is currently no vaccine or therapeutic reagent in clinical use. In this study, we contributed towards the development of a CVB3 vaccine by establishing an efficient and scalable ion exchange chromatography-based purification method for CVB3 virus and baculovirus-insect cell-expressed CVB3 virus-like particles (VLPs). This purification system is especially relevant for vaccine development and produ…
The evolution of collective infectious units in viruses
2019
Viruses frequently spread among cells or hosts in groups, with multiple viral genomes inside the same infectious unit. These collective infectious units can consist of multiple viral genomes inside the same virion, or multiple virions inside a larger structure such as a vesicle. Collective infectious units deliver multiple viral genomes to the same cell simultaneously, which can have important implications for viral pathogenesis, antiviral resistance, and social evolution. However, little is known about why some viruses transmit in collective infectious units, whereas others do not. We used a simple evolutionary approach to model the potential costs and benefits of transmitting in a collect…
Expression and cellular localization of the Nef protein from human immunodeficiency virus-1 in stably transfected B-cells.
1992
Nef protein, encoded by the regulatory nef gene of human immunodeficiency virus type 1 (HIV-1), was expressed in the B-cell line Raji. The cells were stably transfected with plasmids containing the nef transcriptional cassette. They expressed Nef with an Mr of 27,000; the yield could be augmented by incubation with the tumor promoter 12-O-tetradecanoylphorbol-13-acetate. The intracellular localization of Nef was analyzed applying immunofluorescence microscopy using a confocal laser scanning microscope. The antigen was stained with a monoclonal antibody directed against the N-terminal part of Nef. The experiments revealed that in non-dividing cells Nef is present both in the cytoplasm and th…
Análisis filogenético de los genes VP7 y NSP4 de cepas de rotavirus de niños con gastroenteritis en Valencia y Castellón
2012
Las infecciones por rotavirus afectan principalmente en la edad pediátrica y constituyen la primera causa de gastroenteritis en niños. La importancia de estos estudios radica en el conocimiento de las variaciones de los tipos antigénicos circulantes y la aparición o emergencia de nuevos genotipos, lo cual puede influir en las estrategias de vacunación. El G9 de rotavirus genotipo fue reportado por primera vez en 1983 en los Estados Unidos, llegando a ser uno de los genotipos más frecuentes en todo el mundo. La proteína NSP4 tiene un interés especial, ya que tiene un papel importante en el proceso de maduración de los viriones, actuando como receptor intracelular para VP6. Por otra parte, es…
Vitamin D and Osteoporosis in HIV/HCV Coinfected Patients: A Literature Review
2015
Vitamin D deficiency further increases the risk of osteoporosis in HIV-positive patients coinfected with hepatitis C virus (HCV); however, it is still unclear whether HCV-related increased fracture risk is a function of the severity of liver disease. The aim of this review was to identify studies on associative vitamin D deficiency patterns in high-risk populations such as HIV/HCV coinfected patients. We did this by searching MEDLINE and EMBASE databases, from inception to August 2014, and included bibliographies. The final 12 articles selected are homogeneous in terms of age but heterogeneous in terms of sample size, participant recruitment, and data source. Most of the HIV/HCV coinfected …
Neurological manifestations of the West Nile virus infection in some cases found in the clinic of infectious diseases in Sibiu, Romania
2014
Background The neurological manifestations in the West Nile virus infections are present in less than 1% of the diagnosed cases, neurological manifestations which may take many different forms, from encephalitis, meningitis, GuillainBarre syndrome, optic neuritis to polio-like manifestations. We aimed to evaluate the clinical and paraclinical aspects of the cases presenting neurological infections caused by the West Nile virus diagnosed and treated in our clinic.
Booster vaccination after neonatal priming with acellular pertussis vaccine.
2010
After a birth dose of acellular pertussis (aP) and diphtheria (DT)aP-hepatitis B virus (HBV)-inactivated polio vaccine (IPV)/ Haemophilus influenza type b (Hib) at 2, 4, and 6 months, a booster dose of DTaP-HBV-IPV/Hib at 12 to 23 months induced strong anti-pertussis booster responses. Thus, neonatal aP priming did not lead to immune tolerance to pertussis antigens. However, it elicited bystander interference on HBV, Hib, and diphtheria responses.
Innovación docente y TIC desde la perspectiva de la docencia en Derecho
2020
Las tecnologías de la información y la comunicación son un elemento extraordinario para innovar en el ámbito educativo, tal y como ha mostrado la situación generada por el COVID-19. Así, en el ámbito del Derecho son múltiples las posibilidades que se derivan de la implementación de estas herramientas para mejorar el nivel de la enseñanza y facilitar la incorporación de los estudiantes al mundo laboral. Ahora bien, la tecnología no es un fin en sí mismo, por lo que habrá que valorar la introducción de estas herramientas como complemento y no como un sustitutivo de la tarea educativa, especialmente en el ámbito del Derecho, que exige la formación de juristas con una visión integral de la real…
Behind the mask: Rethinking the use of face masks while exercising
2021
there is an ongoing global effort against COVID-19 pan-demic. As vaccination is applied globally (predominantly onat-risk populations), various measures such as temporaryself-quarantine, social distancing, increased hand hygiene,and wearing a face mask (FM) proposed by the World HealthOrganization (WHO) and various governments worldwide arestill in place as primary means of preventing further dissemi-nation. In many instances, wearing a FM became mandatorywhen a person is in a closed space while being accompa-nied by others. Many individuals are therefore apprehensivein terms of maintaining their usual physical activity rout-ines and staying safe in the midst of the outbreak. Asa result, it…
Burden of HIV and hepatitis C co-infection: the changing epidemiology of hepatitis C in HIV-infected patients in France.
2015
Équipe UB/CHU (EA) Pôle MERS CT3 Hors Enjeu; International audience; Background & AimsTo better evaluate the HIV-HCV co-infection burden in the context of new effective HCV treatment. MethodsWe reviewed all the epidemiological data available on HCV-related disease in HIV-infected patients in France. Sources of data have been selected using the following criteria: (i) prospective cohorts or cross-sectional surveys; (ii) conducted at a national level; (iii) in the HIV-infected population; (iv) able to identify HCV co-infection and chronic active hepatitis C (HCV RNA positive); and (v) conducted during the period 2003-2012. ResultsThe overall prevalence of HIV-HCV co-infection has decreased fr…