Search results for "antibody"
showing 10 items of 1896 documents
Enhanced tissue penetration of antibodies through pressurized immunohistochemistry
2020
ABSTRACTTo address the inefficiency of passive diffusion for antibody penetration in thick tissue samples, which limits clearing-technique applications, we developed a versatile and simple device to perform antibody incubation under increased barometric pressure. Pressurized immunohistochemistry greatly improves the uniformity, intensity, and depth of fluorescent immunostaining in thick human and mouse brain samples. Furthermore, pressurized immunohistochemistry substantially decreases the time required for classic staining of thin sections.SUBMISSION CATEGORYNew Results
Blue Fluorescent Exciplexes Consisting oftrans-Stilbene and Antibodies
2001
Cover Picture: Tumor-Associated MUC1 Tandem-Repeat Glycopeptide Microarrays to Evaluate Serum- and Monoclonal-Antibody Specificity (Angew. Chem. Int.…
2009
Achieving dendritic cell subset-specific targeting in vivo by site-directed conjugation of targeting antibodies to nanocarriers
2021
AbstractThe major challenge of nanocarrier-based anti-cancer vaccination approaches is the targeted delivery of antigens and immunostimulatory agents to cells of interest, such as specific subtypes of dendritic cells (DCs), in order to induce robust antigen-specific anti-tumor responses. An undirected cell and body distribution of nanocarriers can lead to unwanted delivery to other immune cell types like macrophages reducing the vaccine efficacy. An often-used approach to overcome this issue is the surface functionalization of nanocarriers with targeting moieties, such as antibodies, mediating cell type-specific interaction. Numerous studies could successfully prove the targeting efficiency…
Modulation of immune parameters of roach, Rutilus rutilus, exposed to untreated ECF and TCF bleached pulp effluents
2000
Abstract The present study was designed to assess the effects of elemental chlorine free (ECF) and totally chlorine free (TCF) pulp and paper mill effluents on the immune defence of the roach ( Rutilus rutilus ). Fish were exposed for 5 weeks to concentrations of 0, 0.6, 2, 6 and 20% of untreated effluents from ECF or TCF bleaching processes. In order to study the capability to respond to foreign antigens the fish were immunised with bovine γ-globulin (BGG) 3 weeks before sampling. The numbers of anti-BGG antibody-secreting cells and immunoglobulin-secreting cells in the spleen and blood, as well as the levels of the anti-BGG antibodies and concentrations of immunoglobulin in plasma and the…
Biosynthesis of the Collagen-like C1q Molecule and its Receptor Functions for Fc and Polyanionic Molecules on Macrophages
1983
At the beginning of the nineteenth century, knowledge of immunity was limited to a few practical methods based on empirical observations, e.g., the observation by Jenner in 1798 that inoculation with cowpox material induced an immunity to smallpox. The discoveries by Louis Pasteur and Robert Koch that microorganisms caused fermentations and were responsible for a number of infectious diseases, greatly advanced the concepts of susceptibility and immunity in a limited number of diseases. In the late nineteenth century, the complement system was discovered by Fodor(1887), Nuttall(1888), and Buchner (1889a, b) through studying the bactericidal action of blood serum. It was recognized that killi…
Differences in Attachment and Phagocytosis of Salmonella minnesota Strains (S Form, Re Mutant) by Mouse Peritoneal Macrophages: Participation of Endo…
1985
The virulence of bacteria in many phagocytic systems has been studied for several years and it is known that macrophages play an important role in host defence against invading microorganisms and in cooperation with cellular and humoral immune mechanisms. Pathogenic bacteria can be divided into two groups with regard to their fate within phagocytes: (a) extracellular bacteria which are promptly killed after phagocytosis and (b) facultative intracellular bacteria which are resistant to intracellular killing unless macrophages are activated. Humoral immune mechanisms (antibody, complement) deal mainly with extracellular bacteria, while cellular immune mechanisms (T cells, macrophages) deal wi…
Ultraviolet B Irradiation Modulates the Immune System of Fish (Rutilus rutilus, Cyprinidae) Part III: Lymphocytes¶
2001
Abstract The effects of short-term exposure to ultraviolet B (UVB) radiation on lymphocyte-related parameters were studied under controlled laboratory conditions using roach (Rutilus rutilus), a cyprinid teleost, as the model fish. In vitro lymphoproliferative responses stimulated with a T-cell–specific mitogen, concanavalin A (ConA), or a B-cell–specific activator, lipopolysaccharide (LPS), were decreased in exposed fish. Also nonstimulated proliferation was lower than in unexposed fish. ConA-activated responses returned to normal levels within 7 days after exposure, but LPS-activated responses were reduced throughout the 14 day follow-up. The capability of UVB-exposed fish to produce an a…