Search results for "braf"

Wnt/β-Catenin dependent roles of the Pygopus family of nuclear factors during Zebrafish early dorso-ventral specification

2014

“THERMAL NOCICEPTION STUDIES IN ZEBRAFISH”

2012

Background Nociception was defined by Sherrington as the mechanism used to sense painful events. Despite the value of current animal models for nociception and pain, we have not yet understood in full how these mechanisms operate. Aim Here we propose a novel approach using zebrafish (Danio rerio) larvae in order to determine if the fish is suitable for the study of pain stimuli. Taking advantage of the fact that, in response to noxious stimuli, the expression of several genes in mammals is induced in neurons of the spinal cord and the dorsal root ganglia (DRG), we investigated if such mechanism is evolutionary conserved in the Zebrafish. Materials and Methods We cloned homologues of mammals…

Optogenetic Interpellation of Behavior Employing Unrestrained Zebrafish Larvae

2017

Phytochemistry of Weigela x "kosteriana variegata" (Caprifoliaceae)

2018

International audience; One new triterpene glycoside 3-O-beta-D-xylopyranosyl-(1 -> 4)-[beta-D-glucopyranosyl-(1 -> 3)]-beta-D-xyl opyranosyl-(1 -> 4)-beta-D-xylopyranosyl-(1 -> 3)-alpha-L-rhamnopyranosyl-(1 -> 2)-alpha-L-arabinopyranosyloleanolic acid, was isolated from Weigela x "kosteriana variegata" (Caprifoliaceae), with three known ones. Their structures were characterized by a combination of mass spectrometry and 1D and 2D NMR spectrocopic techniques including H-1- and C-13 NMR, COSY, TOCSY, NOESY, HSQC, and HMBC experiments. The toxicological properties of some glycosides were determined with a zebrafish-based assay. The results show that the most active compounds were toxic to the …

Role of nitric oxide in tumor development. Role of HSF1 in development of zebrafish embryos in non heat-shocked conditions

2014

The use of animal models has led to the discovery of important mechanisms of biology development in general, and tumor development in particular, to establish and develop new treatments. The Zebrafish (Danio rerio) is increasingly used nowadays as part of this research because of its many advantages such as the transparency of the larvae, and the high homology with human. Several approaches have been developed in this fish, as the gene-knockdown in order to identify the role of a protein in the development, or the tumor transplantation of mammalian cells to study anti-tumor treatments response.It is in one of these two contexts that we studied the role of nitric oxide in tumor development i…

Larval zebrafish as an in vitro model for evaluating toxicological effects of mycotoxins.

2020

The presence of mycotoxins in food has created concern. Mycotoxin prevalence in our environment has changed in the last few years maybe due to climatic and other environmental changes. Evidence has emerged from in vitro and in vivo models: some mycotoxins have been found to be potentially carcinogenic, embryogenically harmful, teratogenic, and to generate nephrotoxicity. The risk assessment of exposures to mycotoxins at early life stages became mandatory. In this regard, the effects of toxic compounds on zebrafish have been widely studied, and more recently, mycotoxins have been tested with respect to their effects on developmental and teratogenic effects in this model system, which offers …

Distinct 5' SCL enhancers direct transcription to developing brain, spinal cord, and endothelium: neural expression is mediated by GATA factor bindin…

1999

The SCL gene encodes a basic helix-loop-helix transcription factor with a pivotal role in the development of endothelium and of all hematopoietic lineages. SCL is also expressed in the central nervous system, although its expression pattern has not been examined in detail and its function in neural development is unknown. In this article we present the first analysis of SCL transcriptional regulation in vivo. We have identified three spatially distinct regulatory modules, each of which was both necessary and sufficient to direct reporter gene expression in vivo to three different regions within the normal SCL expression domain, namely, developing endothelium, midbrain, and hindbrain/spinal …

Study of locomotion response and development in zebrafish (Danio rerio) embryos and larvae exposed to enniatin A, enniatin B, and beauvericin.

2021

Mycotoxins are secondary metabolites produced by a variety of fungi that contaminate food and feed resources, and are capable of inducing a wide range of toxicity. Here, we studied the developmental and behavioral toxicity in zebrafish (Danio rerio) embryos and larvae exposed to three mycotoxins: beauvericin (BEA), Enniatin A (ENN A), and Ennitain B (ENN B). Zebrafish embryos were collected after fertilization, treated individually from 1 to 6 dpf with BEA at 8, 16, 32 and, 64 μM and for both enniatins at 3.12, 6.25, 12.5 and, 25 μM. Mixture of mycotoxins were assayed as follows: i) for BEA + ENN A and BEA + ENN B at [32 + 12.5] μM and [16 + 6.25] μM; ii) for ENN A + ENN B at [12.5 + 12.5] …

Bioconcentration and metabolism of 3,4-dichloroaniline in different life stages of guppy and zebrafish

1993

Abstract Bioconcentration factors (BCF) and metabolism of 3,4-dichloroaniline in different life stages of guppy (Poecilia reticulata Peters ) and zebrafish (Brachydanio rerio Hamilton-Buchanan ) were investigated. The results prove that embryos of zebrafish, four-day-old yolk sac larvae, 17-day-old larvae and adult zebrafish are able to transform the chemical to 3,4-dichloroacetanilide. Bioconcentration factors in these life stages are between 30 and 42, exept yolk sac larvae showing a BCF of 86. 3,4-Dichloroacetanilide was also detected in extracts of newborn and adult guppies, but they are able to form an additional, yet unknown metabolite. The BCF varies from 34 in six-month-old guppies …

Testing Acute Toxicity in the Embryo of Zebrafish, Brachydanio rerio, as an Alternative to the Acute Fish Test: Preliminary Results

1994

Acute toxicity testing in fish is a standard method used in estimating the influences of chemicals on aquatic vertebrates. The ecotoxicological data obtained from acute toxicity tests in fish are, however, not sufficiently reliable to justify the continued use of this test. Fertilised eggs of zebrafish ( Brachydanio rerio) were used to test the acute toxicity of chemicals. They were chosen because the development of B. rerio has been studied extensively and information already exists concerning the normal development of this species. The following parameters of the development of B. rerio were observed: coagulation of the egg, gastrulation, number of somites, movement, development of organ…