Search results for "glia"

Evaluation of gliadins-diglycosylated cyanidins interaction from litchi pericarp through ultraviolet and fluorescence measurements

2017

The low stability of anthocyanins limits their use in industry, which can be surpassed by gliadins linkage. This work was aimed to study the anthocyanins-gliadins bonding properties. HPLC-DAD-ESI-M...

Dual effects of increased glycogen synthase kinase-3β activity on adult neurogenesis

2013

Adult neurogenesis, the generation of new neurons during the adulthood, is a process controlled by several kinases and phosphatases among which GSK3β exerts important functions. This protein is particularly abundant in the central nervous system, and its activity deregulation is believed to play a key role in chronic disorders such as Alzheimer's disease. Previously, we reported that in vivo overexpression of GSK3β (Tet/GSK3β mice) causes alterations in adult neurogenesis, leading to a depletion of the neurogenic niches. Here, we have further characterized those alterations, finding a delay in the switching-off of doublecortin marker as well as changes in the survival and death rates of imm…

Changes in Chemokines and Chemokine Receptors Expression in a Mouse Model of Alzheimer's Disease

2018

The amyloid precursor protein plus presenilin-1 (APP/PS1) mice are a frequently-used model for Alzheimer's disease studies (AD). However, the data relevant to which proteins are involved in inflammatory mechanism are not sufficiently well-studied using the AD mouse model. Using behavioral studies, quantitative RT-PCR and Western-blot techniques, significant findings were determined by the expression of proteins involved in inflammation comparing APP/PS1 and Wild type mice. Increased GFAP expression could be associated with the elevation in number of reactive astrocytes. IL-3 is involved in inflammation and ABDF1 intervenes normally in the transport across cell membranes and both were found …

Ricostruzione virtuale delle crociere dell'aula della chiesa di San Domenico a Cagliari (XVI sec.)

2016

Il saggio affronta la ricostruzione virtuale delle crociere dell’aula della chiesa di San Domenico a Cagliari, distrutta nel 1943 dai bombardamenti alleati che colpirono la città. La ricostruzione virtuale, combinando gli strumenti dell’indagine storica e della rappresentazione digitale, è stata impiegata per comprendere il processo costruttivo della copertura dell’aula. Lo studio ha inoltre permesso di elaborare una più corretta interpretazione dei dettagli linguistici e delle caratteristiche costruttive delle crociere, consentendo di elaborare nuove ipotesi sulla fabbrica, attraverso la comparazione con architetture coeve. This paper focuses on the 3D virtual reconstruction of the star-sh…

Gliadin, zonulin and gut permeabilità: effects on celiac and non-celiac intestinal mucosa and intestinal cell lines.

2006

Objective. Little is known about the interaction of gliadin with intestinal epithelial cells and the mechanism(s) through which gliadin crosses the intestinal epithelial barrier. We investigated whether gliadin has any immediate effect on zonulin release and signaling. Material and methods. Both ex vivo human small intestines and intestinal cell monolayers were exposed to gliadin, and zonulin release and changes in paracellular permeability were monitored in the presence and absence of zonulin antagonism. Zonulin binding, cytoskeletal rearrangement, and zonula occludens-1 (ZO-1) redistribution were evaluated by immunofluorescence microscopy. Tight junction occludin and ZO-1 gene expression …

Mammalian glial cells in culture synthesize acetylcholine.

1997

In the present study we demonstrate that acetylcholine is synthesized by cultured mammalian glial cells identified by cell-type specific markers. Primary cultures of rat brain astrocytes or microglia contained 2.0 and 1.6 pmol acetylcholine/10(6) cells on average respectively. Astrocyte cultures established from neonatal mouse brain contained even more acetylcholine (about 80 pmol acetylcholine/10(6) cells). Primary cultures of rat brain astrocytes showed choline acetyltransferase (ChAT) enzyme activity of 3 nmol/mg protein/h; ChAT activity was blocked by 10 microM bromoacetylcholine. In conclusion, these data demonstrate the synthesis of the "neurotransmitter" acetylcholine in cultured gli…

Chromatin remodelling factor Mll1 is essential for neurogenesis from postnatal neural stem cells

2009

Epigenetic mechanisms that maintain neurogenesis throughout adult life remain poorly understood(1). Trithorax group (trxG) and Polycomb group (PcG) gene products are part of an evolutionarily conserved chromatin remodelling system that activate or silence gene expression, respectively(2). Although PcG member Bmi1 has been shown to be required for postnatal neural stem cell self-renewal(3,4), the role of trxG genes remains unknown. Here we show that the trxG member Mll1 (mixed-lineage leukaemia 1) is required for neurogenesis in the mouse postnatal brain. Mll1-deficient subventricular zone neural stem cells survive, proliferate and efficiently differentiate into glial lineages; however, neur…

Reverse engineering a mouse embryonic stem cell-specific transcriptional network reveals a new modulator of neuronal differentiation

2012

Gene expression profiles can be used to infer previously unknown transcriptional regulatory interaction among thousands of genes, via systems biology 'reverse engineering' approaches. We 'reverse engineered' an embryonic stem (ES)-specific transcriptional network from 171 gene expression profiles, measured in ES cells, to identify master regulators of gene expression ('hubs'). We discovered that E130012A19Rik (E13), highly expressed in mouse ES cells as compared with differentiated cells, was a central 'hub' of the network. We demonstrated that E13 is a protein-coding gene implicated in regulating the commitment towards the different neuronal subtypes and glia cells. The overexpression and …

Cognome familiare

2009

Il nobile casato dei Ventimiglia e Donna Felice Ventimiglia Barberini

2017

Nella prima parte del saggio (R.F. Margiotta, Il nobile casato dei Ventimiglia dalla Liguria alla Sicilia) si delinea la storia dei Ventimiglia, provenienti dalla Liguria, insediatisi in Sicilia sin dal Duecento, da Francesco I a Francesco IV Rodrigo, padre di Felice Ventimiglia, e ci si sofferma sulle opere più rappresentative da loro commissionate. Nella seconda parte (M.C. Di Natale, Felice Ventimiglia Barberini dal principato di Castelbuono a Roma) si prende in esame la figura di donna Felice Ventimiglia e se ne ripercorrono le vicende della vita attraverso due significativi documenti: l'inventario dotale della nobildonna, stilato nel 1693, in occasione delle nozze con il principe di pa…