Search results for "scaffold"
showing 10 items of 470 documents
Bi-layer PCL/PLA scaffold prepared by melt for interface tissue engineering
2017
The development of porous multilayer devices allow controlling chemical, physical and mechanical properties by tuning the properties of each single layer. For instance, this feature is of main concern for the production of porous devices designed to regenerate diseased zones at the interface of tissue presenting intrinsic anisotropic structures that gradually change from one tissue to another. In this context, synthetic biodegradable polymers commonly used biomedical applications include polylactic acid (PLA) and polycaprolactone (PCL). In this work, a novel bi-layered multiphasic scaffold (BLS) is presented. It is composed by a PLA-layer presenting pore size in the range of 90-110 μm while…
Contribution of outgrowth endothelial cells from human peripheral blood on in vivo vascularization of bone tissue engineered constructs based on star…
2009
In the present study we assessed the potential of human outgrowth endothelial cells (OEC), a subpopulation within endothelial progenitor cell cultures, to support the vascularization of a complex tissue engineered construct for bone. OEC cultured on starch polycaprolactone fiber meshes (SPCL) in monoculture retained their endothelial functionality and responded to angiogenic stimulation by VEGF (vascular endothelial growth factor) in fibrin gel-assays in vitro. Co-culture of OEC with human primary osteoblasts (pOB) on SPCL, induced an angiogenic activation of OEC towards microvessel-like structures achieved without additional supplementation with angiogenic growth factors. Effects of co-cul…
Small-angle X-ray scattering reveals compact domain-domain interactions in the N-terminal region of filamin C
2014
Filamins are multi-domain, actin cross-linking, and scaffolding proteins. In addition to the actin cross-linking function, filamins have a role in mechanosensor signaling. The mechanosensor function is mediated by domain-domain interaction in the C-terminal region of filamins. Recently, we have shown that there is a three-domain interaction module in the Nterminal region of filamins, where the neighboring domains stabilize the structure of the middle domain and thereby regulate its interaction with ligands. In this study, we have used small-angle X-ray scattering as a tool to screen for potential domain-domain interactions in the N-terminal region. We found evidence of four domain-domain in…
Magneto-optical nanomaterials: a SPIO–phthalocyanine scaffold built step-by-step towards bimodal imaging
2013
A SPIO-phthalocyanine nanohybrid is developed as a bimodal contrast agent for Optical and Magnetic Resonance Imaging. The organic coating was covalently attached onto SPIO in a step-by-step approach. Each coated-SPIO was thoroughly characterized. The hydrodynamic size of the SPIO-Pc is ca. 60 nm with a coverage of ca. 690 Pc/SPIO.
Scaffold and scaffold-free self-assembled systems in regenerative medicine.
2016
Self-assembly in tissue engineering refers to the spontaneous chemical or biological association of components to form a distinct functional construct, reminiscent of native tissue. Such self-assembled systems have been widely used to develop platforms for the delivery of therapeutic and/or bioactive molecules and various cell populations. Tissue morphology and functional characteristics have been recapitulated in several self-assembled constructs, designed to incorporate stimuli responsiveness and controlled architecture through spatial confinement or field manipulation. In parallel, owing to substantial functional properties, scaffold-free cell-assembled devices have aided in the developm…
Preparation of polymeric foams with a pore size gradient via Thermally Induced Phase Separation (TIPS)
2015
Abstract Foams with a pore size gradient are promising materials for tissue engineering applications where a complex architecture involving morphological variations in space must be mimicked, e.g. in bone tissue repair. In this paper, a technique to obtain a porous scaffold with a pore size gradient is presented. The preparation procedure is based on Thermally Induced Phase Separation (TIPS), by imposing a different thermal history on the two sides of a polymeric solution. In this way, a gradient in thermal history is produced, which will generate a pore size monotonously varying along scaffold thickness. By controlling some parameters easy to manipulate, such as demixing temperature and/or…
Biomaterials and bioactive molecules to drive differentiation in striated muscle tissue engineering
2015
International audience; The generation of engineered tissues and organs has entered into the clinical practice in response to the chronic lack of organ donors. In particular, for the skeletal and cardiac muscles the translational potential of tissue engineering approaches has clearly been shown, even though the construction of these tissues lags behind others given the hierarchical, highly organized architecture of striated muscles. Failure of the cardiac tissue leads to cardiovascular diseases, which are the leading cause of death in the developed world (Di Felice et al., 2014). On the other hand, there are many clinical cases where the loss of skeletal muscle due to a traumatic injury, an…
Optimization of a decellularized protocol of porcine tracheas. Long-term effects of cryopreservation. A histological study
2021
[EN] Objective: The aim of this study was to optimize a decellularization protocol in the trachea of Sus scrofa domestica (pig) as well as to study the effects of long-term cryopreservation on the extracellular matrix of decellularized tracheas. Methods: Porcine tracheas were decellularized using Triton X-100, SDC, and SDS alone or in combination. The effect of these detergents on the extracellular matrix characteristics of decellularized porcine tracheas was evaluated at the histological, biomechanical, and biocompatibility level. Morphometric approaches were used to estimate the effect of detergents on the collagen and elastic fibers content as well as on the removal of chondrocytes from …
Guidance Provided by Teacher and Simulation for Inquiry-Based Learning: a Case Study
2016
Current research indicates that inquiry-based learning should be guided in order to achieve optimal learning outcomes. The need for guidance is even greater when simulations are used because of their high information content and the difficulty of extracting information from them. Previous research on guidance for learning with simulations has concentrated on guidance provided by the simulation. Little research has been done on the role of the teacher in guiding learners with inquiry-based activities using simulations. This descriptive study focuses on guidance provided during small group investigations; pre-service teachers (n = 8) guided third and fifth graders using a particular simulatio…