Search results for "serp"

Giacomo Serpotta tra sacro e profano

2022

We shall be taking an innovative and original approach in our examination of the insuperable figure of Giacomo Serpotta, an outstanding Sicilian stucco sculptor of the 18th century. The complete artistic output of Giacomo Serpotta is traced, oscillating between the sacred and the profane in the sculptural adornment of many churches and oratories. Here, Giacomo Serpotta shifts from the Baroque to Rococo, originating a sculptural art that is profoundly innovative in its language and symbology. Formal reading and chromatic-spatial games are intertwined with the symbolic in the light of significant essays by Carl Gustav Jung, Martin Heidegger and Carlo Giulio Argan

Giacomo Serpotta nella chiesa di Sant'Orsola di Palermo. Studi e restauro

2011

La monografia affronta in maniera interdisciplinare un'opera meno nota di Giacomo Serpotta, realizzata tra il 1695 e il 1696, all'interno della chiesa palermitana di Sant'Orsola. A dispetto dell'apparente consistenza come apparato minore, lo studio e il restauro, intersecando le conoscenze acquisite durante il cantiere, hanno posto in evidenza aspetti meno divulgati sia relativi alla tecnica, che all'iconografia (anche relativamente ad inediti modelli di riferimento), che all'interpretazione delle immagini simboliche. La ricerca, anche con il rinvenimento di documentazione inedita d'archivio, ha fissato le coordinate cronologiche dell'apparato plastico, in relazione al sito e alla produzion…

Co-transmitter mediated facilitation by sympathetic nerve stimulation of evoked acetylcholine release from the rabbit perfused atria preparation.

1995

Rabbit atria were isolated with the extrinsic right sympathetic and vagus nerves attached and perfused with Tyrode solution. Acetylcholine overflow was determined after labelling of the transmitter stores with [14C]choline and fractionation of the radioactivity on cation exchange columns. Sympathetic nerve stimulation (SNS, 2 Hz, 3 min) carried out together with vagus nerve stimulation (VNS, 2 Hz, 3 min), but each SNS pulse preceding a vagal one by 19 ms, caused a facilitation of acetylcholine overflow of about 60% versus independent controls in the absence of SNS. Antagonists of putative neurotransmitters were tested to find out the prejunctional mediator involved in the facilitation. The …

Serpientes y helmintos parásitos en el este peninsular español: la influencia del hábitat y el modo de vida en la helmintofauna de los ofidios ibéric…

2021

Se han analizado helmintológicamente una serie de ejemplares de ofidios terrestres del este peninsular ibérico y los datos obtenidos se han comparado con los de otras serpientes ibéricas y europeas. Se ha detectado una notable pobreza de la helmintofauna de estos ofidios que se explica en términos de sus características bioecológicas, principalmente la estrategia de alimentación y la ocupación de hábitats secos. Snakes and parasitic helminths in Eastern Spain: the influence of habitat and lifestyle on the helminthfauna of Iberian snakes. A number of specimens of terrestrial snakes from the east of the Iberian Peninsula have been helminthologically analyzed and the obtained data were compare…

Quando l'eroe disturba il mostro. Uno studio comparativo sulla figura del mostro-custode: Ḫubaba, Ladone e il serpente della Colchide

2013

Glucosylation of isatin-3-oxime followed by 2D in situ NMR in plant cells at highest magnetic field without labelling.

2001

The glucosylation of isatin-3-oxime (1) was monitored by in situ 2D 1H-13C inverse correlated gradient assisted NMR spectroscopy in plant cell suspension cultures of Rauvolfia serpentina without labelling. The applied high magnetic field of 800 MHz allowed measurements within 20 min at concentrations of 1 of 5.76 mM. Complete glucosylation of 1 occurs inside the cells within 72 hours. During this time isatin-3-oxime-glucoside (2) accumulates without further metabolism.

Heterologous expression of aRauvolfiacDNA encoding strictosidine glucosidase, a biosynthetic key to over 2000 monoterpenoid indole alkaloids

2002

Strictosidine glucosidase (SG) is an enzyme that catalyses the second step in the biosynthesis of various classes of monoterpenoid indole alkaloids. Based on the comparison of cDNA sequences of SG from Catharanthus roseus and raucaffricine glucosidase (RG) from Rauvolfia serpentina, primers for RT-PCR were designed and the cDNA encoding SG was cloned from R. serpentina cell suspension cultures. The active enzyme was expressed in Escherichia coli and purified to homogeneity. Analysis of its deduced amino-acid sequence assigned the SG from R. serpentina to family 1 of glycosyl hydrolases. In contrast to the SG from C. roseus, the enzyme from R. serpentina is predicted to lack an uncleavable N…

High-performance liquid chromatographic, capillary electrophoretic and capillary electrophoretic–electrospray ionisation mass spectrometric analysis …

2002

Systems for efficient separation of selected alkaloid groups by high performance liquid chromatography (HPLC), capillary electrophoresis (CE) and capillary electrophoresis coupled with electrospray ionisation mass spectrometry (CE-ESI-MS) are described. The optimized HPLC system was applied for the separation of 23 standard indole alkaloids as well as for qualitative and quantitative analyses of crude alkaloid extracts of Rauvolfia serpentina X Rhazya stricta hybrid cell cultures. The developed conditions for CE analysis proved to be efficient for separation of mixtures of standard indole and beta-carboline alkaloids. The described buffer system is also applicable in the combination of CE w…

Development of an efficient system for the separation of indole alkaloids by high performance liquid chromatography and its applications

2001

An efficient system for the analysis of indole alkaloids by HPLC on a reversed-phase column using an ion pair technique is described. The optimised chromatographic conditions allowed the successful separation of 22 standard monoterpenoid indole alkaloids (including some isomers) and tryptamine. The described HPLC system was applied to the analysis of alkaloids in intergeneric somatic hybrid cell cultures of Rauvolfia serpentina x Rhazya stricta.

Induction of alkaloid diversity in hybrid plant cell cultures

1999

The treatment of Rauwolfia serpentina x Rhazya stricta somatic hybrid cell suspension culture with 100 μM of methyl jasmonate led to a general increase in indole alkaloid content and to qualitative changes in the alkaloid pattern. The content of Six alkaloids were investigated with respect to their content in both the cell biomass and nutrition medium. Intracellular 17-O-acetyl-norajmaline content on the 5th day after treatment had increased about 40-fold compared with the control culture. The respective concentrations of the other alkaloids increased by a factor of two to five. In total 26 indole alkaloids were identified in extracts of the methyl jasmonate-treated culture by TLC, UV, MS a…