Search results for "uno"
showing 10 items of 14944 documents
The corticotrophin-releasing factor/urocortin system regulates white fat browning in mice through paracrine mechanisms.
2015
Objectives:\ud The corticotrophin-releasing factor (CRF)/urocortin system is expressed in the adipose tissue of mammals, but its functional role in this tissue remains unknown.\ud \ud Methods:\ud Pharmacological manipulation of the activity of CRF receptors, CRF1 and CRF2, was performed in 3T3L1 white pre-adipocytes and T37i brown pre-adipocytes during in vitro differentiation. The expression of genes of the CRF/urocortin system and of markers of white and brown adipocytes was evaluated along with mitochondrial biogenesis and cellular oxygen consumption. Metabolic evaluation of corticosterone-deficient or supplemented Crhr1-null (Crhr1−/−) mice and their wild-type controls was performed alo…
New developments in the pathogenesis of obesity-induced hypertension
2015
Obesity is a disorder that develops from the interaction between genotype and environment involving social, behavioral, cultural, and physiological factors. Obesity increases the risk for type 2 diabetes mellitus, hypertension, cardiovascular disease, cancer, musculoskeletal disorders, chronic kidney and pulmonary disease. Although obesity is clearly associated with an increased prevalence of hypertension, many obese individuals may not develop hypertension. Protecting factors may exist and it is important to understand why obesity is not always related to hypertension. The aim of this review is to highlight the knowledge gap for the association between obesity, hypertension, and potential …
Oligonucleotide-capped nanoporous anodic alumina biosensor as diagnostic tool for rapid and accurate detection of Candida auris in clinical samples.
2021
[EN] Candida auris has arisen as an important multidrug-resistant fungus because of several nosocomial outbreaks and elevated rates of mortality. Accurate and rapid diagnosis of C. auris is highly desired; nevertheless, current methods often present severe limitations and produce misidentification. Herein a sensitive, selective, and time-competitive biosensor based on oligonucleotide-gated nanomaterials for effective detection of C. auris is presented. In the proposed design, a nanoporous anodic alumina scaffold is filled with the fluorescent indicator rhodamine B and the pores blocked with different oligonucleotides capable of specifically recognize C. auris genomic DNA. Gate opening modul…
Valor de las subpoblaciones de las células natural killer y del receptor de citotoxicidad natural NKp44 como biomarcadores de malignidad en derrames …
2015
El derrame pleural maligno es un problema clínico común en los pacientes con enfermedad neoplásica y su aparición es un signo de enfermedad avanzada ya que implica la afectación del espacio pleural por parte de dicho proceso maligno. La citometría de flujo es una técnica que permite un análisis rápido, preciso y reproducible de las células del líquido pleural, pudiendo complementar a la citología en el estudio de los derrames pleurales sospechosos de malignidad. Las células NK están en la primera línea de defensa tumoral, por lo que la determinación de las subpoblaciones de NK, especialmente las que tienen función citotóxica (NK CD56dim CD16-, NK CD56 dim CD16+, NK CD16+ y NK CD57+), en los…
Vapauttava laulupedagogiikka : kriittinen toimintatutkimus oppilaslähtöistä laulunopetusmallia luomassa
2011
Vapauttava laulupedagogiikka on pro gradu -opinnäytetyö, jonka tavoitteena on määritellä laulupedagogista mallia yhdistelemällä oppilaslähtöisiä suuntauksia, kuten psykofyysistä musiikkipedagogiikkaa, Paulo Freiren kriittistä vapautuksen pedagogiikkaa, Eira Korpisen kokonaisvaltaista koulukasvatusmallia, sekä tutkivan opettajan ja oppilaan kokemuksia. Tutkimus on luonteeltaan narratiivinen. Johtopäätöksiä ja tutkimustuloksia ei pidä tulkita yleiseksi totuudeksi, vaan tutkijan subjektiiviseksi näkemykseksi. Lauluopettajien koulutuksessa ei anneta kovin paljon tietoa psykologisten seikkojen vaikutuksesta laulutekniikkaan ja opettamiseen. Tutkielma pyrkii tuottamaan tietoa näistä ilmiöistä esi…
Optimization of the JUNO liquid scintillator composition using a Daya Bay antineutrino detector
2021
To maximize the light yield of the liquid scintillator (LS) for the Jiangmen Underground Neutrino Observatory (JUNO), a 20 t LS sample was produced in a pilot plant at Daya Bay. The optical properties of the new LS in various compositions were studied by replacing the gadolinium-loaded LS in one antineutrino detector. The concentrations of the fluor, PPO, and the wavelength shifter, bis-MSB, were increased in 12 steps from 0.5 g/L and <0.01 mg/L to 4 g/L and 13 mg/L, respectively. The numbers of total detected photoelectrons suggest that, with the optically purified solvent, the bis-MSB concentration does not need to be more than 4 mg/L. To bridge the one order of magnitude in the detect…
Isolated lip dermatitis (atopic cheilitis), successfully treated with topical tacrolimus 0.03%
2020
Background Exfoliative and erosive cheilitis, may be a source of speech and chewing discomfort, but may also be an aesthetic issue for the patients affected. Such a clinical presentation may implicate a variety of inflammatory conditions, including atopic (eczematous) cheilitis. Topical and systemic agents, e.g. corticosteroids, have been used to treat inflammatory lip conditions. Topical tacrolimus has also been used in some inflammatory lip conditions. Material and Methods We performed a retrospective clinical analysis of atopic cheilitis patients. Results Between 2015 and 2020, we addressed 7 (seven) patients with atopic dermatitis affecting only lips and were diagnosed as atopic-eczemat…
Nutritional properties and plausible benefits of Pearl millet (Pennisetum glaucum) on bone metabolism and osteoimmunology : a mini-review
2020
Bone is a hard connective tissue that undergoes a systematic renewal. This highly dynamic organ is made up of four different types of cells, however, bone formation is commonly attributed to osteoblasts and bone resorption to osteoclasts. Bone tissue formation occurs during embryonic development and in certain post-birth pathological conditions. The immune system could influence the functions of bone cells, and the crosstalk between hematopoietic, immune, and bone cells is known as osteoimmunology. Indeed, cytokines produced by immune cells, including TNF-α and IL-6, are critically implicated in bone pathogenesis. It is well established that diet plays an important role in bone health and f…
A novel intrinsically disordered outer membrane lipoprotein ofAggregatibacter actinomycetemcomitansbinds various cytokines and plays a role in biofil…
2017
Intrinsically disordered proteins (IDPs) do not have a well-defined and stable 3-dimensional fold. Some IDPs can function as either transient or permanent binders of other proteins and may interact with an array of ligands by adopting different conformations. A novel outer membrane lipoprotein, bacterial interleukin receptor I (BilRI) of the opportunistic oral pathogen Aggregatibacter actinomycetemcomitans binds a key gatekeeper proinflammatory cytokine interleukin (IL)-1b. Because the amino acid sequence of the novel lipoprotein resembles that of fibrinogen binder A of Haemophilus ducreyi, BilRI could have the potential to bind other proteins, such as host matrix proteins. However, from th…
Oxymetazoline modulates proinflammatory cytokines and the T‐cell stimulatory capacity of dendritic cells
2007
The nasal decongestant oxymetazoline (OMZ) is frequently used in the topical treatment of rhinitis/sinusitis. As proinflammatory cytokines play a critical role in the development and maintenance of local inflammation, the aim of this study was to investigate the influence of OMZ on immune cells in order to diminish the mucosal infiltration of the nose. Peripheral blood mononuclear cells (PBMC) from buffy coats of healthy volunteers were isolated and stimulated in the presence or absence of OMZ. In addition, monocyte-derived dendritic cells (DC) were generated and different concentrations of OMZ were added. DC phenotype and their T-cell stimulatory properties were analysed. The vasoactive su…