0000000000004046
AUTHOR
Paula Montero Magalló
Role of MUC4 in idiopathic pulmonary fibrosis
Background: Idiopathic pulmonary fibrosis (IPF) is a chronic, progressive and irreversible form of fibrotic intersticial lung disease, characterized by uncontrolled fibroblast proliferative processes and alveolar type II epithelial dysfunction. MUC4, a multi-domain transmembrane glycoprotein, is often overexpressed in epithelial cancers, with consequences for the biological properties, involved in cellular processes related to IPF. However, the role of MUC4 in IPF has not beet studied yet. Objective: To analyze the implication of MUC4 in IPF Methods: Lung tissue from 14 healthy and 14 IPF patients was obtained. MUC4 expression was analyzed by western blot, RT-PCR and immunohistochemistry. T…
A preclinical model for skin sensitization prediction of antineoplasic drugs.
e15643 Background: Skin side effects are common manifestations of antineoplastic drugs that are frequently observed in early clinical trials. Therefore, there is a need to identify skin toxic agents before clinical development in order to predict severe skin manifestations. In many cases, skin toxicity is due to sensitization, a key immunologic process mediating redness, swelling and itching that can lead to more severe skin alterations. Methods: We adapted three skin cellular in vitro techniques for cutaneous drug sensitization assessment of the Organization for Economic Cooperation and Development (OECD, 2012) in order to predict antineoplastic drug skin sensitization: 1) Direct peptide …
DPP-4 is overexpressed in lung tissue from idiopathic pulmonary patients and activates lung fibroblasts
Background: Idiopathic pulmonary fibrosis (IPF) is a progressive and irreversible form of fibrotic interstitial lung disease, characterized by uncontrolled fibroblast invasion. Dipeptidyl peptidase-4 (DPP-4)/ glucagon-like peptide 1 (GLP-1) system is involved in multiple effects, including cardiac, liver or kidney fibrosis. However, its implication in IPF has not been described. Objective: To analyse the implication of DPP4/GLP1 system in IPF. Methods: Protein expression of DPP4, GLP-1 and GLP-1 receptor was analyzed in lung tissues from 7 IPF patients. TGFβ1-induced fibroblast to myofibroblast transition (FMT), epithelial to mesenchymal transition (EMT) and mesothelial to mesenchymal trans…
Implantación de modelos tridimensionales de piel humana: Aplicación en la evaluación de los marcadores moleculares de la radicación solar
La piel es el órgano más grande del cuerpo humano y está compuesta por tres capas desde el exterior hasta el interior: epidermis, dermis e hipodermis. Es la primera barrera que protege al cuerpo frente a gran variedad de factores ambientales estresantes, entre ellos, las radiaciones solares. La radiación solar es un espectro de longitudes de onda compuestos por la luz ultravioleta (UV), de la cual llega a la superficie terrestre una combinación de rayos UVB y UVA, el espectro visible (VIS), que tiene un rango que va desde la luz azul en el extremo más corto del espectro, hasta el rojo en el extremo más largo, y la luz infrarroja (IR), que se subdivide en tres regiones (IR-A, IR-B e IR-C). S…