0000000000009637
AUTHOR
Angélique Besson
NO-Based Signaling in Plants
In animals, nitric oxide (NO) is an endogenously produced radical involved in cell communication and signal transduction. Its functions in plants are currently being discovered at an unprecedented pace, and insight into NO-derived mechanisms has mainly been gained from research on signal transduction. Numerous studies have firmly placed NO as one component of the signal perception–transduction network that connects plant responses to primary signals, including hormones, elicitors of defence responses or abiotic stresses. Protein kinases and the second messengers Ca 2+, cGMP, and cADPR convey part of the NO signal within cells. Furthermore, NO-based protein modifications are emerging as broa…
Mechanisms of nitric-oxide-induced increase of free cytosolic Ca2+ concentration in Nicotiana plumbaginifolia cells.
In this study, we investigated a role for nitric oxide (NO) in mediating the elevation of the free cytosolic Ca(2+) concentration ([Ca(2+)](cyt)) in plants using Nicotiana plumbaginifolia cells expressing the Ca(2+) reporter apoaequorin. Hyperosmotic stress induced a fast increase of [Ca(2+)](cyt) which was strongly reduced by pretreating cell suspensions with the NO scavenger carboxy PTIO, indicating that NO mediates [Ca(2+)](cyt) changes in plant cells challenged by abiotic stress. Accordingly, treatment of transgenic N. plumbaginifolia cells with the NO donor diethylamine NONOate was followed by a transient increase of [Ca(2+)](cyt) sensitive to plasma membrane Ca(2+) channel inhibitors …
Nitric oxide signalling in plants: interplays with Ca2+ and protein kinase
International audience; Much attention has been paid to nitric oxide (NO)research since its discovery as a physiological mediator of plant defence responses. In recent years, newer roles have been attributed to NO, ranging from root development to stomatal closure. The molecular mechanisms underlying NO action in plants are just begun to emerge. The currently available data illustrate that NO can directly influence the activity of target proteins through nitrosylation and has the capacity to act as a Ca2+-mobilizing intracellular messenger. The interplay between NO and Ca2+ has important functional implications, expanding and enriching the possibilities for modulating transduction processes…