0000000000010238
AUTHOR
María Del Carmen Caba Pérez
Evaluation of dynamic microbial communities in a styrene-degrading biotrickling filter using 16S rDNA tag pyrosequencing and denaturing gradient gel electrophoresis
Accurately characterizing microbial communities within bioreactors undergoing dynamic operating conditions is an essential first step towards understanding the relationship between microbial community structure and bioreactor performance. A detailed assessment of the changes in microbial populations within a styrene-degrading biotrickling filter was carried out using samples collected at multiple time points ranging from 21 to 155 days of biotrickling filter operation. Examination of microbial populations was conducted by 16S rDNA tag pyrosequencing and denaturing gradient gel electrophoresis (DGGE). Validation of pyrosequencing results was performed by quantitative polymerase chain reactio…
Removal of 2-butoxyethanol gaseous emissions by biotrickling filtration packed with polyurethane foam
The removal of 2-butoxyethanol from gaseous emissions was studied using two biotrickling filters (BTF1 and BTF2) packed with polyurethane foam. Two different inoculum sources were used: a pure culture of Pseudomonas sp. BOE200 (BTF1) and activated sludge from a municipal wastewater treatment plant (BTF2). The bioreactors were operated at inlet loads (ILs) of 130 and 195 g m(-3) hour(-1) and at an empty bed residence time (EBRT) of 12.5s. Under an IL of ∼130 g m(-3) hour(-1), BTF1 presented higher elimination capacities (ECs) than BTF2, with average values of 106±7 and 68±8 g m(-3) hour(-1), respectively. However, differences in ECs between BTFs were decreased by reducing the irrigation inte…
Evolution of Bacterial Community in a Full-scale Biotrickling Filter by Fluorescence in Situ Hybridization (FISH)
The performance of a full-scale biotrickling system for the treatment of exhaust gases from two different paint sources at a furniture facility, was investigated applying Fluorescense in situ hybridization (FISH). This technique allowed the detection of major bacteria groups and, therefore, helped in understanding complex microbial communities. The results indicated that Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, and Deltaproteobacteria were more predominant than Firmicutes and Actiniobacterias. In addition, a variation in the composition of the bacterial community throughout the time of operation and with the paint source was observed. Betaproteobacteria showed similar r…
Study of Mass Oxygen Transfer in a Biotrickling Filter for Air Pollution Control
Biotrickling filtration is a potential and cost effective alternative for the treatment of volatile organic compound (VOC) emissions in air, so it is necessary to deepen into the key aspects of design and operation for the optimization of this technology. One of these factors is the oxygen mass transfer of the process. This study would facilitate the selection of the packing material and the mathematical modelling and simulation of bioreactors. Four plastic packing materials with a different specific surface area have been evaluated in terms of oxygen mass transfer. For the tested range of superficial liquid velocities, data show a relationship between the kLa and the superficial liquid vel…
Abatement of styrene waste gas emission by biofilter and biotrickling filter: comparison of packing materials and inoculation procedures.
The removal of styrene was studied using 2 biofilters packed with peat and coconut fibre (BF1-P and BF2-C, respectively) and 1 biotrickling filter (BTF) packed with plastic rings. Two inoculation procedures were applied: an enriched culture with strain Pseudomonas putida CECT 324 for biofilters and activated sludge from a municipal wastewater treatment plant for the BTF. Inlet loads (ILs) between 10 and 45 g m-3 h-1 and empty bed residence times (EBRTs) from 30 to 120 s were applied. At inlet concentrations ranging between 200 and 400 mg Nm-3, removal efficiencies between 70 and 95% were obtained in the 3 bioreactors. Maximum elimination capacities (ECs) of 81 and 39 g m-3 h-1 were obtained…
Evaluation of a pilot-scale biotrickling filter as a VOC control technology for the plastic coating sector
Abstract The performance and feasibility of a biotrickling filter (BTF) pilot unit for the treatment of exhaust gases from two robotic spray paint booths at a plastic coating facility were investigated. The volatile organic compound (VOC) concentrations in the emissions of the exhaust gases from the paint booths were relatively stable, although the VOC composition depended on the applied solvent-paint formulation in the booths. The pilot plant was operated for one year at empty bed residence times (EBRTs) ranging from 30 to 93 s. The performance of the system was affected by the solvent-paint formulations. An EBRT between 30 and 40 s was enough to meet legal requirements for products contai…
Comparison of inoculums in the removal of 2-butoxyethanol from air emissions by biotrickling filter: Performance and microbial monitoring
2-butoxyethanol is one of the most used glycol ether in industrial activities and the treatment of air 2-butoxyethanol-emissions become necessary. Biotechnologies are potential treatment technologies due to their low operational costs. The use of two inoculums in the treatment of 2-butoxyethanol by biotrickling filters (BTFs) packed with polyurethane-foam was studied. A pure culture of Pseudomonas putida, previously adapted to 2-butoxyethanol, was used as inocula in a BTF operated in the University of Stuttgart. Fresh activated sludge from a municipal waste water treatment plant was used as inocula in a BTF operated in the University of Valencia. An empty bed residence time of 12.5 s and in…