Detection of ephemeral genetic sub-structure in the narrow endemic Abies nebrodensis (Lojac.) Mattei (Pinaceae) using RAPD markers
A. nebrodensis (Nebrodi fir, Sicilian fir) is restricted to a small area of the Madonie Natural Park in Sicily. According to recent estimates, its only population consists of 30 adult individuals and a fluctuating number of juveniles derived from natural regeneration; besides, some hundreds of cultivated plants are preserved as ex situ collection. We used RAPD data from six 10-mer primers to examine the consequences of extensive historical clearance and human pressures on the extant population. Data from multiple life stages and different habitat conditions were considered, affording an opportunity to ascertain for the first time the structure of genetic variation in the extant uneven-aged …
A reassessment of the sections of the genus Cytisus Desf. (Cytiseae, Leguminosae)
Recent morphological and molecular research on Cytisus and allied genera has produced a great deal of new data relevant to systematics, which have not yet been incorporated into a consistent classification system of the genus. We have compared and evaluated recently published cladograms and phenograms based on morphological and molecular (nuclear and plastid DNA) characters. The genus Cytisus sensu lato, including Calicotome, Chamaecytisus, Chronanthus, and Lembotropis, appears to be monophyletic. A subdivision of the genus Cytisus in 13 sections is presented; one species, C. tribracteolatus, remains incertae sedis. A new section (C. sect. Dendrocytisus) and two new combinations for taxa in…
Isolation and biodiversity inCytisus villosusPourret (Fabaceae, Genisteae): enzyme polymorphism in disjunct populations
ABSTRACT The genetic diversity of isolated populations of Cytisus villosus has been studied by means of enzyme polymorphism analysis. Two types of isolated populations were studied: “terrestrial islands” in Sicily, and “true islands” in the Aeolian archipelago. In the populations of “true islands” the number of alleles and the heterozygosity are lower than in “terrestrial islands”. Isolation amongst Sicilian populations seems to be more recent than isolation of the Aeolian populations, and may be attributed to climatic changes which occurred during the Holocene and/or to human activities. The disjunction of the Aeolian populations seems much more recent than the origin of the isles themselv…
Genetic diversity in Cytisus aeolicus Guss. (Leguminosae), a rare endemite of the Italian flora
ABSTRACT Cytisus aeolicus Guss, is an endemic plant restricted to the isles of Vulcano, Stromboli and Alicudi in the Aeolian archipelago. All known populations were assayed for genetic variability using enzyme polymorphism. Allozyme variation at 16 loci coding for 10 enzyme systems was examined. The great majority of loci turned out to be monomorphic or fixed heterozygous. The observed genetic depauperation is indicative of historical factors, such as the bottleneck effect associated with migration, and the founder effect in population re-establishment. The low genetic diversity is largely partitioned within rather than among populations, indicating that extant populations have not been est…