Inverse correlated 1H-13C in vivo NMR as a probe to follow the metabolism of unlabeled vanillin by plant cell cultures

Abstract Inverse correlated 2D 1H-13C NMR spectroscopy was used to follow the metabolism of vanillin (1) in vivo by plant cell suspension cultures of Nicotiana plumbaginifolia at the natural abundance of 13C. Under the non-invasive conditions four products could be detected: vanillylalcohol (2), vanillinglucoside (3) and the phenolic and benzylic glucosides of vanillylalcohol (4 and 5).