0000000000020699
AUTHOR
R. Dolcemascolo
Actinorhodin production intensification by nanofibrous membranes in Streptomyces coelicolor cultures
In this work, electrospun polycaprolactone (PCL) and polylactic acid (PLA) membranes, subjected or not to O2-plasma treatment, werwe used as support for cell-immobilization in S. coelicolor immobilized-cells created a compact biofilm on both kinds of membranes.
Enhancement of antibiotic productions by plasma modified PLA electrospun membranes in Streptomyces coelicolor immobilized-cell cultivations
This study, encouraging the use of PLA membranes for actinomycetes cultivations, could unveil functional insights associated with antibiotic production and S. coelicolor cellimmobilization
Improvement of actinorhodin production yield in Streptomyces coelicolor by immobilized-cell cultivations by using PCL- and PLA-based films
Actinomycetes are Gram-positive bacteria producing most of naturally occurring antibiotics (Donadio et al., 2010). At industrial level, antibiotics are produced by submerged fermentations where the actinomycete filamentous morphology negatively affects bioproductivity (van Dissel et al., 2014). Microporous membranes for bacterial cell-immobilization were already proven increasing bioproductivity in Streptomyces coelicolor, that is a model actinomycete producing the blue pigmented actinorhodin (ACT) antibiotic (Scaffaro et al., 2016). To develop an immobilized-cell bioreactor system, different kinds of polycaprolactone (PCL) and polylactic acid (PLA) films were produced by an electrospinning…
Effect of Streptomyces coelicolor M145 cell immobilization on actinorhodin production
Non previsto
IMPROVED PRODUCTION OF THE ANTIBIOTIC ACTINORHODIN IN STREPTOMYCES COELICOLOR IMMOBILIZED-MYCELIAL CELL CULTIVATIONS
Objectives i) Evaluation of ACT production in Streptomyces coelicolor M145 mycelial cells immobilized on polycaprolactone (PCL) and polylactic acid (PLA) nanofiber membranes, modified or not by an O2- plasma treatment. ii) Identification of gene products associated with the improvement of ACT production.