0000000000020918

AUTHOR

Minako Ishibashi

showing 6 related works from this author

Liver X Receptor Regulates Arachidonic Acid Distribution and Eicosanoid Release in Human Macrophages

2013

Objective— Liver X receptors (LXRs) are oxysterol-activated nuclear receptors that are highly expressed in macrophages and regulate lipid homeostasis and inflammation. Among putative LXR target genes, lysophosphatidylcholine acyltransferase 3 (LPCAT3) involved in the Lands cycle controls the fatty acid composition at the sn-2 position of glycerophospholipids and, therefore, the availability of fatty acids, such as arachidonic acid (AA), used for eicosanoid synthesis. The aim of our study was to determine whether LXRs could regulate the Lands cycle in human macrophages, to assess the consequences in terms of lipid composition and inflammatory response, and to work out the relative contribut…

InflammationBiologySensitivity and SpecificityDinoprostoneMonocyteschemistry.chemical_compoundDownregulation and upregulationmedicineHumansDimethyl SulfoxideRNA MessengerLiver X receptorReceptorCells CulturedLiver X ReceptorsInflammationArachidonic AcidMacrophagesLysophospholipid acyltransferase activity1-Acylglycerophosphocholine O-AcyltransferaseMicroarray AnalysisOrphan Nuclear ReceptorsUp-RegulationchemistryEicosanoidNuclear receptorBiochemistryEicosanoidslipids (amino acids peptides and proteins)Arachidonic acidmedicine.symptomCardiology and Cardiovascular MedicineArteriosclerosis, Thrombosis, and Vascular Biology
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Reduced VLDL clearance in ApoeNpc1 mice is associated with increased Pcsk9 and Idol expression and decreased hepatic LDL-receptor levels

2010

Niemann-Pick type C1 (NPC1) promotes the transport of LDL receptor (LDL-R)-derived cholesterol from late endosomes/lysosomes to other cellular compartments. NPC1-deficient cells showed impaired regulation of liver_X receptor (LXR) and sterol regulatory element-binding protein (SREBP) target genes. We observed that Apoe(-/-)Npc1(-/-) mice displayed a marked increase in total plasma cholesterol mainly due to increased VLDL, reflecting decreased clearance. Although nuclear SREBP-2 and Ldlr mRNA levels were increased in Apoe(-/-)Npc1(-/-) liver, LDL-R protein levels were decreased in association with marked induction of proprotein convertase subtilisin/kexin type 9 (Pcsk9) and inducible degrade…

Apolipoprotein EreceptorCholesterol VLDLLDL/metabolismMacrophages Peritoneal/cytologyBiochemistryMiceEndocrinologyhemic and lymphatic diseasesReceptorsOrphan Nuclear Receptors/geneticspolycyclic compoundsnuclear receptorCells CulturedResearch ArticlesLiver X ReceptorsMice KnockoutCulturedSterol Regulatory Element Binding Protein 2/geneticslipoproteinSerine EndopeptidasesIntracellular Signaling Peptides and ProteinsLamin Type AOrphan Nuclear ReceptorsTriglycerides/bloodCholesterolLiverProteins/geneticsKexinlipids (amino acids peptides and proteins)Proprotein ConvertasesProprotein Convertase 9Sterol Regulatory Element Binding Protein 1Niemann-Pick diseaseSterol Regulatory Element Binding Protein 2medicine.medical_specialtyCellsKnockoutUbiquitin-Protein LigasesReceptors LDL/metabolismSerine Endopeptidases/geneticsQD415-436BiologyCholesterol/blooddigestive systemApolipoproteins ELiver/physiologySterol Regulatory Element Binding Protein 1/geneticsNiemann-Pick C1 ProteinInternal medicinemedicineAnimalsPeritoneal/cytologyCholesterol VLDL/metabolismUbiquitin-Protein Ligases/geneticsLiver X receptorTriglyceridesMacrophagesPCSK9Proteinsnutritional and metabolic diseasesVLDL/metabolismLamin Type A/metabolismCell BiologySterol regulatory element-binding proteinEndocrinologyReceptors LDLLDL receptorMacrophages PeritonealSterol regulatory element-binding protein 2atherosclerosisApolipoproteins E/geneticsLipoproteinJournal of Lipid Research
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Identification of biological markers of liver X receptor (LXR) activation at the cell surface of human monocytes.

2012

Background Liver X receptor (LXR) α and LXR β (NR1H3 and NR1H2) are oxysterol-activated nuclear receptors involved in the control of major metabolic pathways such as cholesterol homeostasis, lipogenesis, inflammation and innate immunity. Synthetic LXR agonists are currently under development and could find applications in various fields such as cardiovascular diseases, cancer, diabetes and neurodegenerative diseases. The clinical development of LXR agonists requires the identification of biological markers for pharmacodynamic studies. In this context, monocytes represent an attractive target to monitor LXR activation. They are easily accessible cells present in peripheral blood; they expres…

Hydrocarbons FluorinatedCD226Celllcsh:MedicineBiochemistryMonocytesDrug DiscoveryMolecular Cell Biologypolycyclic compoundsSignaling in Cellular Processeslcsh:ScienceLiver X ReceptorsSulfonamidesMultidisciplinarymedicine.diagnostic_testfood and beveragesCell DifferentiationOrphan Nuclear ReceptorsFlow CytometryNuclear SignalingCholesterolmedicine.anatomical_structureGene Knockdown Techniqueslipids (amino acids peptides and proteins)Research ArticleSignal TransductionAgonistmedicine.drug_classImmune CellsImmunologyContext (language use)Biologydigestive systemFlow cytometryAntigens CDDNA-binding proteinsmedicineHumansRNA MessengerLiver X receptorBiologyCluster of differentiationMacrophagesCell Membranelcsh:RProteinsLipid MetabolismMetabolismGene Expression RegulationNuclear receptorImmunologyCancer researchlcsh:QBiomarkersCytometryFoam CellsDevelopmental BiologyPLoS ONE
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Liver X receptor activation promotes polyunsaturated fatty acid synthesis in macrophages : relevance in the context of atherosclerosis

2015

Objective— Liver X receptors (LXRs) modulate cholesterol and fatty acid homeostasis as well as inflammation. This study aims to decipher the role of LXRs in the regulation of polyunsaturated fatty acid (PUFA) synthesis in macrophages in the context of atherosclerosis. Approach and Results— Transcriptomic analysis in human monocytes and macrophages was used to identify putative LXR target genes among enzymes involved in PUFA biosynthesis. In parallel, the consequences of LXR activation or LXR invalidation on PUFA synthesis and distribution were determined. Finally, we investigated the impact of LXR activation on PUFA metabolism in vivo in apolipoprotein E–deficient mice. mRNA levels of acyl…

Context (language use)Biologydigestive systemchemistry.chemical_compoundMicearachidonic acidAnimalsHumansFatty acid homeostasisReceptorLiver X receptor[ SDV.BBM ] Life Sciences [q-bio]/Biochemistry Molecular BiologyLiver X Receptorschemistry.chemical_classificationCholesterolFatty acidfood and beveragesArteriesAtherosclerosisOrphan Nuclear ReceptorsmacrophagesBiochemistrychemistryn-3 polyunsaturated fatty acidFatty Acids UnsaturatedArachidonic acidlipids (amino acids peptides and proteins)Cardiology and Cardiovascular MedicineSterol Regulatory Element Binding Protein 1Polyunsaturated fatty acidFoam Cellsliver X receptor
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Knock-down of the oxysterol receptor LXRα impairs cholesterol efflux in human primary macrophages: lack of compensation by LXRβ activation.

2012

Liver X Receptors (LXRs) α and β are oxysterol-activated nuclear receptors involved in the control of lipid metabolism and inflammation. Pharmacological activation of LXR is promising in the treatment of atherosclerosis since it can promote cholesterol efflux from macrophages and prevent foam cell formation. However, the development of LXR agonists has been limited by undesirable side-effects such as hepatic steatosis mediated by LXRα activation. Therefore, it has been proposed that targeting LXRα activators to extrahepatic tissues or using LXRβ-specific activators could be used as alternative strategies. It is not clear whether these molecules will retain the full atheroprotective potentia…

Apolipoprotein Emedicine.medical_specialtyBenzylaminesOxysterolHydrocarbons FluorinatedPrimary Cell CultureBiochemistryBenzoatesApolipoproteins EInternal medicinemedicineHumansRNA Small InterferingReceptorLiver X receptorCells CulturedFoam cellLiver X ReceptorsPharmacologySulfonamidesbiologyApolipoprotein A-IMacrophagesOrphan Nuclear ReceptorsLipoproteins HDL2Cell biologyEndocrinologyCholesterolABCG1Nuclear receptorABCA1Gene Knockdown Techniquesbiology.proteinlipids (amino acids peptides and proteins)Biochemical pharmacology
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Activation of liver x receptors promotes polyunsaturated fatty acid synthesis and eicosanoid secretion in human macrophages

2014

chemistry.chemical_classificationchemistryBiochemistryEicosanoid secretionCardiology and Cardiovascular MedicineLiver X receptorPolyunsaturated fatty acidAtherosclerosis
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