0000000000027119
AUTHOR
Clémence Thiour Mauprivez
Membre du comité d'organisation logistique et scientifique
visioconférence, France, 6-9 octobre 2020; International audience
DIAGSOL : development of a new functional marker of exposure to herbicides B-triketones in an agricultural soil
Numerous herbicides target an enzyme found not only in weeds but also in « non-target organisms » such as microorganisms. This proof-of-concept study aims to use microbial gene encoding the targeted enzyme or the targeted enzyme itself as a marker for herbicide exposure in soils. The hppd gene and the encoded enzyme (HPPD; 4-hydroxyphenylpyruvate dioxygenase), targeted by B-triketone herbicides, are the subject of this study. In silico analyses reveal that the hppd gene is spread out in all bacterial phyla. Primers specific to this gene were designed. This primer pair is used to measure the abundance, the composition and the diversity of the hppd bacterial community in soil microcosms expos…