0000000000054734

AUTHOR

Detlef Jung

showing 3 related works from this author

mRNA Induction and Cytokine Release of Inflammatory Mediators During In Vitro Exposure of Human Nasal Respiratory Epithelia to Acetaldehyde

2006

Acetaldehyde has been shown to be cytotoxic and carcinogenic to the upper respiratory tract epithelium of rodents following long-term exposure. Most animal studies have concentrated on carcinogenicity and DNA-protein cross-link formation, while less is known about potential dose- and time-dependent induction of aldehyde-induced rhinitis in humans. In this in vitro study, 22 primary cell cultures established from inferior turbinate tissue of healthy individuals were exposed to acetaldehyde concentrations of 50 (German MAK value) or 500 ppm for 4 or 24 h. mRNA expression and protein levels of cytokines and other inflammatory mediators were quantified at the end of the 4- and 24-h exposures. C…

Health Toxicology and Mutagenesismedicine.medical_treatmentAcetaldehydeRespiratory MucosaNoseBiologyToxicologychemistry.chemical_compoundmedicineHumansRNA MessengerCells CulturedMonocyteAcetaldehydeInterleukinReal-time polymerase chain reactionmedicine.anatomical_structureCytokineGene Expression RegulationchemistryCell cultureImmunologyCytokinesTumor necrosis factor alphaInflammation MediatorsRespiratory tractInhalation Toxicology
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mRNA-induction and cytokine release during in vitro exposure of human nasal respiratory epithelia to methyl methacrylate

2007

Abstract Background Methyl methacrylate (MMA) has been reported to cause histopathological changes in rodent nasal epithelium after inhalation challenges. Data in humans are lacking. Methods In this in vitro design 22 primary cell cultures taken from inferior turbinate tissue of healthy individuals were exposed to MMA concentrations of 50 ppm (German MAK-value) and 200 ppm. mRNA expression and cytokine release of inflammatory mediators were quantified after 4 h and after 24 h. Controls were exposed to synthetic air. Q-PCR analysis was performed for TNF-α, IL-1β, IL-6, IL-8, MCP-1, GMCSF, Cox-1 and Cox-2. ELISA assays were performed from culture supernatants for TNF-α, IL-1β, IL-6, IL-8, MCP…

Cell Survivalmedicine.medical_treatmentCell Culture TechniquesEnzyme-Linked Immunosorbent AssayInflammationMethylmethacrylateBiologyToxicologyAndrologyDownregulation and upregulationmedicineHumansRNA MessengerRespiratory systemCells CulturedChemokine CCL2Dose-Response Relationship DrugReverse Transcriptase Polymerase Chain ReactionTumor Necrosis Factor-alphaInterleukinsGranulocyte-Macrophage Colony-Stimulating FactorAntimutagenic AgentsEpithelial CellsGeneral MedicineEpitheliumIn vitroNasal MucosaDose–response relationshipCytokinemedicine.anatomical_structureGene Expression RegulationCyclooxygenase 2Cell cultureImmunologyCyclooxygenase 1Cytokinesmedicine.symptomToxicology Letters
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Early stress response of human nasal respiratory epithelia after exposure to 1-methoxypropanol-2

2007

To evaluate the impact of 1-methoxypropanol-2 (MEP) for the stimulation of an inflammatory response in human respiratory mucosa, we exposed 22 primary cell cultures of nasal respiratory epithelia of healthy individuals to MEP concentrations at the level of the German MAK-value (100 ppm) and to the 10-fold concentration (1000 ppm). After 4 and 24h we analyzed the transcription of TNF-alpha, IL-1beta, IL-6, IL-8, MCP-1, GMCSF, Cox-1 and Cox-2 by quantitative PCR as well as the release of the respective cytokines by ELISA. At both MEP concentrations we observed a significant increase of TNF-alpha-, IL-1beta-, IL-6- and Cox-2-transcripts after 4h. After 24h cytokine transcription of TNF-alpha, …

Respiratory Mucosamedicine.medical_specialtyTranscription Geneticmedicine.medical_treatmentInflammationStimulationAir Pollutants OccupationalBiologyGranulocyteToxicologyProinflammatory cytokineInternal medicinemedicineHumansRNA MessengerRespiratory systemCells CulturedGeneral MedicineNasal MucosaCytokineEndocrinologymedicine.anatomical_structurePropylene GlycolsImmunologySolventsCytokinesmedicine.symptomRespiratory tractToxicology Letters
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