0000000000076273
AUTHOR
Jose S. Vicente
Direct transfer of vitrified rabbit embryos.
In this study we looked at the feasibility of transferring vitrified rabbit embryos directly into recipient does. Compacted morulae were vitrified in a solution of 20% ethylene glycol and 20% dimethyl sulfoxide. After thawing, and without step-wise diluted solution, the vitrified embryos were transferred into the recipient's uterine horns. Survival rate at birth differed from fresh rabbit embryos (40% vs 55%, P0.05). However, the percentage of does that delivered (94%) and the survival rate suggested this method is suitable for both storage and simple transfer of rabbit morulae.
Osmotic and cryoprotective effects of a mixture of DMSO and ethylene glycol on rabbit morulae.
Abstract Comparisons were made of the osmotic and cryoprotective effects on rabbit embryos preserved by vitrification with 2 solutions and by conventional freezing. Embryos obtained from rabbits killed 70 to 72 h after mating were used in the study (n = 948). Initially, toxicity of the 3 cryoprotectants was studied in fresh (unfrozen) embryos (n = 135). Subsequently, embryos placed in ethylene glycol (EG, 40% v/v; n = 88) and ethylene glycol with dimethyl sulfoxide (EG+DMSO, 20% v/v each, respectively; n = 344) were loaded into straws and plunged directly into liquid nitrogen. Embryos placed in 1.5 M DMSO and 20% heat inactivated rabbit serum were subjected to conventional freezing in a pro…
Oviductal and endometrial mRNA expression of implantation candidate biomarkers during early pregnancy in rabbit
[EN] Prenatal losses are a complex problem. Pregnancy requires orchestrated communication between the embryo and the uterus that includes secretions from the embryo to signal pregnancy recognition and secretion and remodelling from the uterine epithelium. Most of these losses are characterized by asynchronization between embryo and uterus. To better understand possible causes, an analysis was conducted of gene expression of a set of transcripts related to maternal recognition and establishment of rabbit pregnancy (uteroglobin, SCGB1A1; integrin 1, ITGA1; interferon- , IFNG; vascular endothelial growth factor, VEGF) in oviduct and uterine tissue at 16, 72 or 144 h post-ovulation and insemina…