0000000000083896

AUTHOR

Amparo Tamarit

showing 6 related works from this author

Assessment of human cytomegalovirus specific T cell immunity in human immunodeficiency virus infected patients in different disease stages following …

2004

T cell immunity to human cytomegalovirus (HCMV) was assessed in HAART-treated HIV-1 infected patients (9 asymptomatic, CDC group A; and 22 symptomatic, CDC group B), and in eight HIV-1 long term non-progressors. Patients were either prospectively or cross-sectionally examined for CD4(+) T cell counts, HIV RNA load, HCMV leukoDNAemia, HCMV DNA in urine, lymphoproliferative response (LPR) to HCMV and phytohemagglutinin (PHA), and cytokine secretion (IFN-gamma and IL-4) by HCMV-stimulated peripheral blood mononuclear cell (PBMC) cultures. No patient either progressed to clinical AIDS or developed HCMV active infection during the study period. Twenty-nine patients responded to HAART, though 12 …

Human cytomegalovirusAdultMaleAdolescentvirusesT cellT-LymphocytesCongenital cytomegalovirus infectionCytomegalovirusViremiaHIV InfectionsBiologyIn Vitro TechniquesLymphocyte ActivationPeripheral blood mononuclear cellAsymptomaticHIV Long-Term SurvivorsVirologyAntiretroviral Therapy Highly ActivemedicineHumansvirus diseasesbiochemical phenomena metabolism and nutritionMiddle Agedmedicine.diseaseVirologyInfectious Diseasesmedicine.anatomical_structureImmunologyCytokinesCytokine secretionFemalemedicine.symptomLymphoproliferative responseJournal of medical virology
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Human cytomegalovirus (HCMV)-specific CD4+ T lymphocyte response in AIDS patients with no past or current HCMV disease following HAART.

2003

Abstract Background: The incidence of Human Cytomegalovirus (HCMV) end-organ disease has dramatically decreased since the implementation of highly active antiretroviral therapies (HAARTs), but the precise immune mechanism whereby HCMV is controlled remains to be elucidated. Objectives: To investigate the effect of (HAART) on CD4 + T-cell immunity to HCMV in AIDS patients with no past or current HCMV disease. Study design: Seventeen patients were prospectively examined for CD4 + (CD45RO + and CD45 RA + ) T-cell counts (flow cytometry), HIV RNA load (Amplicor HIV test), HCMV leukoDNAemia and HCMV DNA in urine (nested PCR), lymphoproliferative response (LPR) to HCMV, phytohemagglutinin (PHA) a…

Human cytomegalovirusAdultCD4-Positive T-LymphocytesMalevirusesCytomegalovirusmedicine.disease_causeLymphocyte ActivationHerpesviridaeVirusInterferon-gammaBetaherpesvirinaeT-Lymphocyte SubsetsVirologyImmunopathologyAntiretroviral Therapy Highly ActivemedicineHumansViremiaAcquired Immunodeficiency SyndromebiologyAIDS-Related Opportunistic Infectionsvirus diseasesHIVbiochemical phenomena metabolism and nutritionMiddle AgedViral Loadbiology.organism_classificationmedicine.diseaseVirologyCD4 Lymphocyte CountInterleukin-10Infectious DiseasesImmunologyCytomegalovirus InfectionsDNA ViralCytokinesRNA ViralCytokine secretionFemaleViral diseaseInterleukin-4Lymphoproliferative responseJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology
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Early neutralizing and glycoprotein B (gB)-specific antibody responses to human cytomegalovirus (HCMV) in immunocompetent individuals with distinct c…

2000

Abstract Background: Antibodies with functional anti-Human Cytomegalovirus (HCMV) activity are likely to be involved in preventing virus dissemination and thus may contribute to minimize the clinical manifestations of infection. Objectives: To investigate the role of humoral immunity in modulating the clinical expression of primary Human Cytomegalovirus (HCMV) infection in immunocompetent persons. Study design: Neutralizing (NA) and glycoprotein B (gB)-specific antibodies were quantitated in acute-phase and late-convalescence phase sera from 19 individuals who developed either HCMV mononucleosis (12) or oligosymptomatic hepatitis (seven). Results: The levels of NA in sera drawn early after …

AdultMaleHuman cytomegalovirusAdolescentHepatitis Viral HumanMononucleosisvirusesAntibody AffinityCongenital cytomegalovirus infectionBiologyAntibodies ViralViral Envelope ProteinsNeutralization TestsVirologymedicineHumansAvidityInfectious MononucleosisChildmedicine.diseaseVirologyInfectious DiseasesChild PreschoolImmunoglobulin GCytomegalovirus InfectionsDNA ViralHumoral immunityImmunologybiology.proteinFemaleAntibodyViral hepatitisImmunocompetenceViral loadJournal of Clinical Virology
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Longitudinal analysis of human cytomegalovirus glycoprotein B (gB)-specific and neutralizing antibodies in AIDS patients either with or without cytom…

2001

Serum neutralizing and glycoprotein B (gB)-specific antibody levels were monitored prospectively in AIDS patients who either did or did not develop human cytomegalovirus (HCMV) end-organ disease, to delineate further the role of antibodies in protecting against HCMV disease. Antibody levels declined substantially (at least 4-fold) only in patients who developed HCMV disease; this decline in turn occurred concurrently with antigenemia. Nevertheless, AIDS patients who remained free of HCMV disease and did not become antigenemic during the follow-up period maintained stable levels of serum antibodies, with only minor fluctuations. The impact of HAART on the levels of functional anti-HCMV antib…

Human cytomegalovirusbiologybusiness.industryvirusesAntibody titerCongenital cytomegalovirus infectionvirus diseasesmedicine.disease_causemedicine.diseasebiology.organism_classificationVirologyHerpesviridaeInfectious DiseasesBetaherpesvirinaeVirologyImmunopathologyImmunologymedicinebiology.proteinViral diseaseAntibodybusinessJournal of Medical Virology
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Effect of highly active anti-retroviral therapy and hepatitis C virus co-infection on serum levels of pro-inflammatory and immunoregulatory cytokines…

2006

ABSTRACTThis study aimed to determine the effect of highly active anti-retroviral therapy (HAART) and hepatitis C virus (HCV) co-infection on peripheral levels of interleukin (IL)-2, IL-10, IL-12 (p70), IL-18 and soluble tumour necrosis factor receptor type II (sTNFRII). Serum levels were monitored for a 1-year period in 25 patients infected with human immunodeficiency virus-1 (HIV-1) who were naive for HAART at the initiation of the study, and in four HIV-1-infected long-term non-progressors. Serum levels of both IL-18 and sTNFRII at baseline were significantly higher in HIV-1-infected patients than in controls. Baseline levels of IL-18 and sTNFRII were not significantly different in long-…

AdultMaleMicrobiology (medical)hepatitis C virusTime FactorsHAARTHepatitis C virusHepacivirusHIV Infectionsmedicine.disease_causeserum cytokinesReceptors Tumor Necrosis FactorVirusEtanerceptAntiretroviral Therapy Highly ActiveImmunopathologymedicineHumansLongitudinal StudiesDemographybiologyhuman immunodeficiency virusbusiness.industryInterleukinsInterleukinvirus diseasesGeneral MedicineHepatitis Csoluble tumour necrosis factor receptor type IIMiddle Agedmedicine.diseasebiology.organism_classificationHepatitis CInfectious DiseasesImmunoglobulin GImmunologyLentivirusHIV-1CytokinesFemaleViral diseasebusinessIL-18Clinical Microbiology and Infection
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Application of a 5-bromo-2′-deoxyuridine ELISA for measuring the lymphoproliferative response to human cytomegalovirus in HIV-1-infected patients

2002

Assessment of the lymphoproliferative response to human cytomegalovirus (HCMV) may help to identify human immunodeficiency virus (HIV)-1-infected patients at high risk of developing HCMV end-organ disease. The tritiated thymidine ([3H]-TdR)-incorporation assay is the gold standard for measuring lymphoproliferative responses, though it is unsuitable as a routine laboratory procedure. An alternative non-radioactive technique, a 5-bromo-2'-deoxyuridine (BrdU) enzyme-linked immunosorbent assay, was applied for measuring T-cell proliferation in response to HCMV. Stimulation of either 1 x 10(5) or 5 x 10(4) peripheral blood mononuclear cells (PBMCs)/well with 10 PFU/well (before inactivation) of …

Human cytomegalovirusCellular immunityvirusesCytomegalovirusEnzyme-Linked Immunosorbent AssayBiologyLymphocyte ActivationPeripheral blood mononuclear cellViruschemistry.chemical_compoundVirologymedicineHumansAcquired Immunodeficiency SyndromeAIDS-Related Opportunistic Infectionsvirus diseasesmedicine.diseaseVirologyDeoxyuridineBromodeoxyuridinechemistryCytomegalovirus InfectionsHIV-1Indicators and ReagentsThymidineLymphoproliferative responseBromodeoxyuridineJournal of Virological Methods
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