DOTAGA-Trastuzumab. A New Antibody Conjugate Targeting HER2/Neu Antigen for Diagnostic Purposes.
International audience; Improved bifunctional chelating agents (BFC) are required for indium-111 radiolabeling of monoclonal antibodies (mAbs) under mild conditions to yield stable, target-specific agents. 2,2',2″-(10-(2,6-Dioxotetrahydro-2H-pyran-3-yl)-1,4,7,10-tetraazacyclododecane-1,4,7-triyl)triacetic acid (DOTAGA-anhydride) was evaluated for mAb conjugation and labeling with indium-111. The DOTA analogue was synthesized and conjugated to trastuzumab-which targets the HER2/neu receptor-in mild conditions (PBS pH 7.4, 25 °C, 30 min) and gave a mean degree of conjugation of 2.6 macrocycle per antibody. Labeling of this immunoconjugate with indium-111 was performed in 75% yield after 1 h a…
Multi-modal image fusion for small animal studies in in-line PET /3T MRI
Congrès sous l’égide de la Société Française de Génie Biologique et Médical (SFGBM).; National audience; In the framework of small animal multi-modal imaging, the current progression of the IMAPPI project is illustrated by the design of an in-line PET/MRI prototype, coupled to a dedicated multi-resolution registration method allowing the robust fusion of data coming from both modalities. The first results show a good alignment of the data from tumor imaging at the level of the abdomen.
Integrated PET/MRI in preclinical studies State of the art
International audience; The exquisite tissue contrast of magnetic resonance imaging (MRI), the absence of ionising radiation and the opportunity to obtain new molecular and functional data have strengthened the enthusiasm for coupling MRI rather than computed tomography (CT) to positron emission tomography (PET). When reviewing the current literature one might be surprised by the almost unlimited diversity of what is placed under the name of PET/MRI in the articles. The magnetic field is varying from 0.3 Tesla (T) to 9.4 T, the size of the bore varies also from the wide bore of clinical scanners to volumes limited to a few tens of mL. Many preclinical studies are performed using separate PE…
Temozolomide and radiotherapy antitumor efficacy evaluation with magnetic resonance imaging and proton magnetic resonance spectroscopy in human glioma models in nude rats
International audience
Dimensionality Reduction Techniques: An Operational Comparison On Multispectral Satellite Images Using Unsupervised Clustering
Multispectral satellite imagery provides us with useful but redundant datasets. Using Dimensionality Reduction (DR) algorithms, these datasets can be made easier to explore and to use. We present in this study an objective comparison of five DR methods, by evaluating their capacity to provide a usable input to the K-means clustering algorithm. We also suggest a method to automatically find a suitable number of classes K, using objective "cluster validity indexes" over a range of values for K. Ten Landsat images have been processed, yielding a classification rate in the 70-80% range. Our results also show that classical linear methods, though slightly outperformed by more recent nonlinear al…
MR-based biomarkers in the diagnosis and the evaluation of the therapeutic response to radiotherapy (ETRR) in prostatic carcinoma (PCa): Implementation of clinical and experimental approaches
e16136 Background: ETRR in PCa remains a challenging task. New biomarkers in the form of metabolites, detected with Proton Magnetic Resonance Spectroscopy (1H-MRS), and quantitative Apparent Diffusion Coefficient (ADC), determined with Diffusion-Weighted Magnetic Resonance Imaging, could be of value in the ETRR for PCa. Methods: Twenty-one men with biopsy proven localized PCa were examined before external radiotherapy. 3D-1H-MRS was performed at 3.0T without endorectal coils. The delivered radiotherapy dose was 78 Gy in 39 fractions with 6 MV photons. MR follow-up examinations up to 36 months post-radiotherapy were performed. For the pre-clinical study, healthy rat prostate was studied in …