0000000000084393

AUTHOR

Domenico Puzzolo

showing 3 related works from this author

Caspase 9 and Caspase 3 Immunohistochemical Pattern in Skeletal and Cardiac Muscles at Different Times after Death: An Experimental Study on PMI Esti…

2021

(1) Background: The estimation of the post mortem interval (PMI) is a challenge for forensic pathologists because data emerging from methods commonly applied are not always conclusive, since several conditions exist that may affect the reliability of these parameters. Thus, new approaches have been proposed to overcome such a limit. In recent years, several studies have been performed on proteins analyzing their expression/degradation patterns in relation to the progressing of the post mortem interval. (2) Methods: The immunoreactivity patterns of two apoptosis mediators—Caspase 9 and Caspase 3—have been tested in order to evaluate their potential role as markers of the post mortem interval…

Pathologymedicine.medical_specialtyMedicine (General)cardiac muscleforensic sciencesClinical BiochemistryCaspase 3Article03 medical and health sciences0302 clinical medicineR5-920caspase 3Medicine030216 legal & forensic medicineskeletal musclecaspase 9Caspase030304 developmental biologyPost-mortem intervalCaspase-90303 health sciencespost mortem intervalbiologybusiness.industryCardiac muscleSkeletal musclemedicine.anatomical_structureApoptosisbiology.proteinImmunohistochemistrybusinesstime after deathDiagnostics
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Post-Mortem Immunohistochemical Evidence of β2-Adrenergic Receptor Expression in the Adrenal Gland

2019

The evidence from post-mortem biochemical studies conducted on cortisol and catecholamines suggest that analysis of the adrenal gland could provide useful information about its role in human pathophysiology and the stress response. Authors designed an immunohistochemical study on the expression of the adrenal &beta

Male0301 basic medicinePathologyGene ExpressionStimulationlcsh:Chemistry0302 clinical medicineCortex (anatomy)Adrenal Glandspost-mortem analysisReceptorlcsh:QH301-705.5SpectroscopyCause of deathAged 80 and overAdrenal glandβ2-adrenergic receptorsGeneral MedicineMiddle AgedPathophysiologyComputer Science Applicationsmedicine.anatomical_structureimmunohistochemistryImmunohistochemistryFemaleAutopsyAdultmedicine.medical_specialtyAdolescentBiologyArticleCatalysisInorganic ChemistryYoung Adult03 medical and health sciencesSettore MED/43 - Medicina LegalemedicineHumans030216 legal & forensic medicinepost-mortem analysiPhysical and Theoretical ChemistryMolecular BiologyAgedadrenal glandOrganic Chemistry030104 developmental biologylcsh:Biology (General)lcsh:QD1-999adrenal gland; immunohistochemistry; post-mortem analysis; β2-adrenergic receptorsReceptors Adrenergic beta-2HormoneInternational Journal of Molecular Sciences
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Structural, ultrastructural, and morphometric study of the zebrafish ocular surface: a model for human corneal diseases?

2018

Purpose: A morphological and morphometric study of the adult zebrafish ocular surface was performed to provide a comprehensive description of its parts and to evaluate its similarity to the human. Materials and Methods: The eyes of adult zebrafish were processed for light, transmission and scanning electron microscopy, and for immunohistochemical stain of corneal nerves; a morphometric analysis was also performed on several morphological parameters. Results: The corneal epithelium was formed by five layers of cells. No Bowman’s layer could be demonstrated. The stroma consisted of lamellae of different thickness with few keratocytes. The Descemet’s membrane was absent as the flat and polygon…

0301 basic medicineBowman's layer; corneal nerves; Descemet's membrane; ocular surface; Zebrafish; Ophthalmology; Sensory Systems; Cellular and Molecular NeuroscienceConjunctivaCorneal StromaBiologycorneal nerveCorneal DiseasesCornea03 medical and health sciencesCellular and Molecular Neuroscience0302 clinical medicineMicroscopy Electron TransmissionCorneamedicineAnimalsHumansTrigeminal NerveBowman MembraneZebrafishDescemet MembraneZebrafishTrigeminal nerveocular surfaceBowman’s layerCorneal DiseasesEndothelium CornealEpithelium CornealDescemet’s membraneEpithelial CellsAnatomybiology.organism_classificationSensory SystemsDescemet's membraneOphthalmology030104 developmental biologymedicine.anatomical_structureModels Animal030221 ophthalmology & optometryUltrastructureMicroscopy Electron ScanningGoblet CellsBowman MembraneConjunctivaCurrent eye research
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