0000000000116705

AUTHOR

Angelo Rizzo

showing 2 related works from this author

Outbreak of ST395 KPC-Producing Klebsiella pneumoniae in a Neonatal Intensive Care Unit in Palermo, Italy

2018

0301 basic medicineMicrobiology (medical)Klebsiella pneumoniae Neonatal Intensive Care Unit Carbapenem resistance KPC Outbreakmedicine.medical_specialtyNeonatal intensive care unitEpidemiologyKlebsiella pneumoniae030106 microbiologySettore MED/42 - Igiene Generale E Applicatabeta-Lactam ResistanceDisease Outbreaks03 medical and health sciencesIntensive Care Units NeonatalmedicineHumansBacteriological TechniquesInfection ControlbiologyOutbreakbiology.organism_classificationKlebsiella InfectionsKlebsiella pneumoniae030104 developmental biologyInfectious DiseasesCarbapenemsItalyEmergency medicineBeta lactam antibiotics
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In vitro generation of CD4+CD25+ regulatory cells from murine naive T cells

2007

CD4+ CD25+ regulatory T cells (Tregs) are crucial for the maintenance of immunological tolerance. Recent data indicate that Tregs not only develop in the thymus during ontogeny but can also differentiate from naive T cells in the periphery. The following protocol describes a method by which Tregs are generated in vitro by stimulation of naive T cells in the presence of transforming growth factor beta (Ti-Tregs). In vitro-induced regulatory T cells express markers of conventional Treg such as CD25 and the genetic program committing transcription factor FoxP3. Functionally the in vitro-generated Ti-Tregs suppress T-cell activation and proliferation while in vivo these cells have been proven t…

CD4-Positive T-LymphocytesCD3 ComplexT-Lymphocytesmedicine.medical_treatmentchemical and pharmacologic phenomenaBiologyBioinformaticsT-Lymphocytes RegulatoryGeneral Biochemistry Genetics and Molecular BiologyMiceInterleukin 21CD28 AntigensmedicineAnimalsCytotoxic T cellIL-2 receptorInterleukin 3Mice Inbred BALB CInterleukin-2 Receptor alpha SubunitFOXP3hemic and immune systemsTransfectionImmunotherapyTransforming growth factor betaCell biologyCD4 Antigensbiology.proteinBiomarkersNature Protocols
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