0000000000133773

AUTHOR

Céline Segafredo

showing 1 related works from this author

Evaluation of whole antioxidant defenses of human mononuclear cells by a new in vitro biological test: lack of correlation between erythrocyte and mo…

2009

1873-2933 (Electronic) Journal Article; OBJECTIVES: This work aims to evaluate the resistance of mononuclear cells to oxidative stress using a "KRL" test, formerly utilized to evaluate the resistance of erythrocyte to free radicals. METHODS: The "KRL" test evaluates the resistance to lysis of cells treated by free radicals generated under standardized conditions. RESULTS: We defined new analytical parameters (level of radical production, time course, number of cells) to obtain an accurate assay determining the resistance to oxidative stress of mononuclear cells, in comparison to that of erythrocytes. This test allows the evaluation of change in the redox state of mononuclear cells (improved…

AntioxidantLysisErythrocytesmedicine.medical_treatmentRadicalClinical BiochemistryStatistics as TopicCell Culture TechniquesAntimycin AInflammationImmunologic Testsmedicine.disease_causePeripheral blood mononuclear cellAntioxidantsAnti-Bacterial Agents/pharmacologyImmunologic Tests/*methodsElectron Transport Complex IIIReactive Oxygen Species/metabolismAntioxidants/*metabolism/pharmacologymedicineErythrocytes/*metabolismLeukocytesHumansDiagnosticOxidative Stress/*drug effectschemistry.chemical_classificationReactive oxygen speciesAntimycin A/pharmacologyElectron Transport Complex III/antagonists & inhibitorsChemistryMononuclear/drug effects/*metabolismReproducibility of ResultsGeneral MedicineIn vitroAnti-Bacterial AgentsOxidative StressBiochemistryLeukocytes MononuclearReagent KitsReagent Kits Diagnosticmedicine.symptomReactive Oxygen SpeciesOxidation-ReductionOxidative stress
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