A 3D‑scaffold of PLLA induces the morphological differentiation and migration of primary astrocytes and promotes the production of extracellular vesicles

The present study analyzed the ability of primary rat astrocytes to colonize a porous scaffold, mimicking the reticular structure of the brain parenchyma extracellular matrix, as well as their ability to grow, survive and differentiate on the scaffold. Scaffolds were prepared using poly-L-lactic acid (PLLA) via thermally-induced phase separation. Firstly, the present study studied the effects of scaffold morphology on the growth of astrocytes, evaluating their capability to colonize. Specifically, two different morphologies were tested, which were obtained by changing the polymer concentration in the starting solution. The structures were characterized by scanning electron microscopy, and a…

Effects of different ras mutations on colorectal cancer cells

Prevalence of Anaplasma, Ehrlichia, Rickettsia and Coxiella in different tick species

Caratterizzazione dell'antigene AMA1 in ceppi di Babesia bigemina isolati in Italia

Babesia bigemina is an endemic parasite in different parts of the world, including Europe and the Americas. One of the few genes characterized in this species codifies for the apical membrane antigen 1 (AMA-1), a transmembrane antigen recently identified. We characterized the ama-1 gene from three Italian B. bigemina strains. Italian sequences were compared to those of the Australian strain. The results obtained confirmed that this newly described ama-1 gene is highly conserved among Italian and foreign strains, which has implications for vaccine development.

A retrospective study of the characterization of Rickettsia species in ticks collected from humans

Rickettsiae (family Rickettsiaceae, order Rickettsiales) are obligate intracellular bacteria transmitted by arthropod vectors. Several Rickettsia species causing vector-borne rickettsioses belong to the spotted fever group (SFG). Traditionally, Rickettsia conorii has been considered as the main etiologic agent of Mediterranean spotted fever. However, the molecular characterization of rickettsiae allowed identifying other species involved in spotted fever in the Mediterranean region. In this study, 42 ticks collected from humans were subjected to morphological identification and molecular characterization of Rickettsia species potentially involved in human rickettsiosis in Sicily. Fourteen t…

Serological and Molecular Evidence of Bartonella henselae in Stray Cats from Southern Italy

Bartonella henselae is a slow growing and facultative intracellular pathogen mainly transmitted by arthropod vectors adapted to domestic and wild mammalian reservoir hosts. Since cats are the major source of the B. henselae infection, this study aimed to evaluate the seroprevalence and the DNA presence in randomly sampled stray cats. Blood samples of 429 cats were collected from shelter of Palermo (Southern Italy) and sera and whole blood were analyzed for the presence of antibodies against B. henselae by indirect immunofluorescence assay (IFA) and by real-time polymerase chain reaction (PCR), respectively. Two hundred and three sera (47.3%) were positive to IFA and 148 blood samples (34.5%…

Easy detection and differentiation of Anaplasma ovis and Anaplasma marginale by PCR based on MSP4 gene

Gene expression profiling of HT-29 cells in response to induction of oncogenic H and K-ras.

SVILUPPO DI METODICHE PER LA DIAGNOSI DELLA BABESIOSI BOVINA E RICERCA DI CANDIDATI VACCINALI IN BABESIA BIGEMINA

Cattle babesiosis is a tick-borne disease transmitted by haemoparasites belonging to the phylum of Apicomplexa, such as Babesia bovis and B.bigemina. The pathology affects cattle mainly in tropical and subtropical areas, but also in Europe, strongly reducing meat and milk production. The study performed during these three years of PhD was addressed to the molecular characterization of Italian B.bigemina strains isolated from infected animals, with attention to the genes codifying for surface antigens, putative candidates for vaccine and diagnostic tools development. One of the molecules potentially involved in the erythrocyte invasion by B.bigemina is the Apical Membrane Antigen-1 (AMA-1), …

Molecular characterization of B. bovis Merozoite surface antigen 2c (Msa2c) from Italian strains

Effescts of different RAS mutations on colorectal cancer cells

Immune response to tick-borne hemoparasites: Host adaptive immune response mechanisms as potential targets for therapies and vaccines

This article belongs to the Section Molecular Immunology.

Babesia bigemina Apical Membrane Antigen-1: features of the gene and predictive information of the protein from Italian strains

Babesia bigemina is a tick-borne haemoparasite affecting cattle in different parts of the world. One of the few antigens characterized in this species codifies for the Apical Membrane Antigen 1 (AMA-1), a transmembrane protein recently identified. In this work, we characterized the ama-1 gene from four Italian B.bigemina strains, two obtained from Ragusa, Sicily (ITA1 and ITA3), one obtained from Benevento, Campania (ITA2) and one coming from Perugia, Umbria (ITA4). Italian sequences were compared to the Australian strain, whose sequence is available in the Sanger Institute web site, and to some strains coming from different parts of the world. This study provides useful information on the …

Recent Advances on the Innate Immune Response to Coxiella burnetii.

Coxiella burnetii is an obligate intracellular Gram-negative bacterium and the causative agent of a worldwide zoonosis known as Q fever. The pathogen invades monocytes and macrophages, replicating within acidic phagolysosomes and evading host defenses through different immune evasion strategies that are mainly associated with the structure of its lipopolysaccharide. The main transmission routes are aerosols and ingestion of fomites from infected animals. The innate immune system provides the first host defense against the microorganism, and it is crucial to direct the infection towards a self-limiting respiratory disease or the chronic form. This review reports the advances in understanding…

Microscopic evidence of the primary astrocytes' morphological differentiation and migration inside porous Poly-L-lactic acid 3D‑scaffolds

Tissue engineering is an emerging multidisciplinary field that aims at reproducing in vitro and/or in vivo tissues with morphological and functional features similar to the biological tissue of the human body [1]. In the attempt to construct suitable tissue models, a critical step is the setting of 3D scaffolds that mimic the supportive structures of a natural extracellular matrix microenvironment into which cells are normally embedded. In this context, the generation of 3D cultures of brain cells is of particular interest. For instance, the poly L‐lactic acid (PLLA) polymer is wildly used because of its biocompatible and biodegradable potential; the PLLA scaffold topography simulates the n…

Development and validation of two PCR tests for the detection of and differentiation between Anaplasma ovis and Anaplasma marginale

Anaplasma ovis and Anaplasma marginale are tick-transmitted bacteria that cause anaplasmosis in domestic and wild animals. Recent results show that some domestic and wild animals and ticks are susceptible to both A. ovis and A. marginale, thus supporting the need to differentiate between these species in hosts and ticks diagnosed with Anaplasma infection. However, although anaplasmosis is one of the most common diseases of grazing animals worldwide, rapid and effective tests are not available for the detection of and discrimination between these 2 Anaplasma species. The objective of this research was to develop an easy and reliable method to identify and discriminate between the closely rel…

Innate immune response to tick-borne pathogens: Cellular and molecular mechanisms induced in the hosts

This article belongs to the Special Issue Inflammasome.

Rickettsia conorii Indian Tick Typhus Strain and R. slovaca in Humans, Sicily

Letter to the Editor.-- et al.

Scalp eschar and neck lymphadenopathy caused by Rickettsia massiliae

To the Editor: Scalp eschar and neck lymphadenopathy is a common clinical entity that most frequently affects women and children during spring and fall. It is usually caused by Rickettsia slovaca and R. raoultii. Typical clinical signs are a scalp lesion at the tick bite site and regional, often painful, lymphadenopathy. Acute disease can be followed by residual alopecia at the bite site (1,2). Two designations have been proposed for this syndrome: tick-borne lymphadenopathy and Dermacentor-borne necrosis-erythema-lymphadenopathy (both have been associated with R. slovaca); however, the most generic and all-inclusive term is scalp eschar and neck lymphadenopathy. R. massiliae belongs to the…