Detection of Mycobacterium tuberculosis complex in cattle by PCR on milk, lymphnode and nasal swabs.

The PCR technique was applied to the diagnosis of tuberculosis in live cattle, and both skin-test-negative and skin-test-positive animals were studied. DNA was taken from various sources including specimens of lymph node aspirates, milk, and nasal swabs. After slaughter and visual inspection, tissues such as lymph nodes, lungs, and udders from tuberculin reactors were tested by the same technique. Specific oligonucleotide primers internal to the IS6110 insertion element were used to amplify a 580-bp fragment. A 182-bp fragment was obtained by designating a nested PCR from the first amplification product. This fragment was cloned and sequenced, and after being labeled it was employed in dot …

Soil and Regulated Deficit Irrigation Affect Growth, Yield and Quality of ‘Nero d’Avola’ Grapes in a Semi-Arid Environment

The present work studied the effect of two consecutive years of regulated deficit irrigation (RDI) compared to rain fed management on the vegetative growth, yield, and quality of ‘Nero d’Avola’ grapes. The trial was conducted separately in two soils (vertisol and entisol) located at the top and bottom hillside of the same vineyard. Vertisol was characterized by greater depth, organic matter, exchangeable K20, and total N than entisol. RDI was based on an irrigation volume at 25% of estimated crop evapotranspiration (ETc) up to end of veraison and 10% of estimated ETc up to 15 days before harvest. Predawn water potential (PDWP) was used as indicator of plant water status and irrigation timin…

AlKam Metabolic Syndrome Study (AlkaMeSy Study): Studio di prevalenza sulla sindrome metabolica.

Analysis of seroprevalence against [i]Coxiella burnetii[/i] in a sample of farm workers in Western Sicily

[b]Introduction and objective. [/b]Little is known about the development of chronic Q fever caused by [i]Coxiella burnetii [/i]in occupational risk groups and in the general population in Italy, as well as in many countries in the world. The aim of this study was to highlight the presence of the infection in a sample of workers operating outdoors (but not directly in contact with animals), in three provinces of western Sicily, in order to detect the human seroprevalence and compare the obtained data with those found in animals raised in the same territory. [b]Materials and methods.[/b] The study included 126 generic seasonal agricultural workers (labourers), 84 male and 42 female; none of w…