0000000000139738
AUTHOR
Franziska Krajinski
Mtha1, a Plasma Membrane H+-ATPase Gene fromMedicago truncatula,Shows Arbuscule-Specific Induced Expression in Mycorrhizal Tissue
: Transport processes between plant and fungal cells are key elements in arbuscular mycorrhiza (AM), where H+-ATPases are considered to be involved in active uptake of nutrients from the symbiotic interface. Genes encoding H+-ATPases were identified in the genome of Medicago truncatula and three cDNA fragments of the H+-ATPase gene family (Mtha1 - 3) were obtained by RT-PCR using RNA from M. truncatula mycorrhizal roots as template. While Mtha2 and Mtha3 appeared to be constitutively expressed in roots and unaffected by AM development, transcripts of Mtha1 could only be detected in AM tissues and not in controls. Further analyses by RT-PCR revealed that Mtha1 transcripts are not detectable …
Construction and validation of cDNA-based Mt6k-RIT macro- and microarrays to explore root endosymbioses in the model legume Medicago truncatula
To construct macro- and microarray tools suitable for expression profiling in root endosymbioses of the model legume Medicago truncatula, we PCR-amplified a total of 6048 cDNA probes representing genes expressed in uninfected roots, mycorrhizal roots and young root nodules [Nucleic Acids Res. 30 (2002) 5579]. Including additional probes for either tissue-specific or constitutively expressed control genes, 5651 successfully amplified gene-specific probes were used to grid macro- and to spot microarrays designated Mt6k-RIT (M. truncatula 6k root interaction transcriptome). Subsequent to a technical validation of microarray printing, we performed two pilot expression profiling experiments usin…
Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile
Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments…
Genome of an arbuscular mycorrhizal fungus provides insight into the oldest plant symbiosis
International audience; The mutualistic symbiosis involving Glomeromycota, a distinctive phylum of early diverging Fungi, is widely hypothesized to have promoted the evolution of land plants during the middle Paleozoic. These arbuscular mycorrhizal fungi (AMF) perform vital functions in the phosphorus cycle that are fundamental to sustainable crop plant productivity. The unusual biological features of AMF have long fascinated evolutionary biologists. The coenocytic hyphae host a community of hundreds of nuclei and reproduce clonally through large multinucleated spores. It has been suggested that the AMF maintain a stable assemblage of several different genomes during the life cycle, but thi…