0000000000150255
AUTHOR
M. Azam Mansoor
A simple high-performance liquid chromatography (HPLC) method for the measurement of pyridoxal-5-phosphate and 4-pyridoxic acid in human plasma.
Abstract Background Low concentration of plasma pyridoxal-5-phosphate (PLP) is associated with hyperhomocysteinemia and inflammation. Most methods for the measurement of plasma PLP require large specimen volume and involve the use of toxic reagents. Methods We have developed a HPLC method for the measurement of PLP and 4-pyridoxic acid (4-PA) in plasma, which requires small specimen volume. The samples are prepared without adding any toxic reagents. Furthermore, we have examined whether intake of vitamin B 6 affects the concentration of plasma PLP and 4-PA. Results The coefficient of variation of the method was 6% and the recovery of the added vitamin in plasma was about 100%. The concentra…
Effects of polymorphisms in endothelial nitric oxide synthase and folate metabolizing genes on the concentration of serum nitrate, folate, and plasma total homocysteine after folic acid supplementation: a double-blind crossover study.
Abstract Objectives A number of studies have explored the effects of dietary nitrate on human health. Nitrate in the blood can be recycled to nitric oxide, which is an essential mediator involved in many important biochemical mechanisms. Nitric oxide is also formed in the body from l -arginine by nitric oxide synthase. The aim of this study was to investigate whether genetic polymorphisms in endothelial nitric oxide synthase (eNOS) and genes involved in folate metabolism affect the concentration of serum nitrate, serum folate, and plasma total homocysteine in healthy individuals after folic acid supplementation. Methods In a randomized double-blind, crossover study, participants were given …
Effect of genetic polymorphisms involved in folate metabolism on the concentration of serum folate and plasma total homocysteine (p-tHcy) in healthy subjects after short-term folic acid supplementation: a randomized, double blind, crossover study
Data on the effect of combined genetic polymorphisms, involved in folate metabolism, on the concentration of serum folate after folic acid supplementation are scarce. Therefore, we investigated the impact of seven gene polymorphisms on the concentration of serum folate and p-tHcy in healthy subjects after short-term folic acid supplementation. In a randomized, double blind, crossover study, apparently healthy subjects were given either 0.8 mg folic acid per day (n = 46) or placebo (n = 45) for 14 days. The washout period was 14 days. Fasting blood samples were collected on day 1, 15, 30 and 45. Data on subjects on folic acid supplementation (n = 91) and on placebo (n = 45) were used for the…
Higher leukocyte subpopulation counts in healthy smoker industrial workers than in nonsmoker industrial workers: possible health consequences.
<b><i>Objectives:</i></b> Cigarette smoke contains free radicals, which cause injury to endothelial cells and oxidize bioactive components in the blood. Neutrophils, a subpopulation of leukocytes, contain the enzyme myeloperoxidase that mediates production of hypochlorous acid during oxidative stress. In this study, we investigated whether smoker industrial workers had significantly higher neutrophil counts than nonsmoker industrial workers. <b><i>Design and Methods:</i></b> We collected blood samples from 183 apparently healthy male and 30 female industrial workers. We obtained blood cell counts, measured the concentration of plasma aminothio…
Early Biochemical and Hematological Response to Intramuscular Cyanocobalamin Therapy in Vitamin B<sub>12</sub>-Deficient Patients
<b><i>Background:</i></b> Data on early biochemical and hematological responses to cobalamin therapy in vitamin B<sub>12</sub>-deficient patients are scarce. Therefore, we investigated whether cobalamin injections would include prompt biochemical and hematological responses in vitamin B<sub>12</sub>-deficient patients. <b><i>Subjects and Methods:</i></b> Seven female patients (mean age: 69.4 years, range: 61-78) with a mean serum cobalamin level of 104 ± 38 pmol/l mean ± SD and 7 male patients (mean age: 67.0 years, range: 53-78) with a mean serum cobalamin level of 84 ± 40 (±SD) participated in the study. They were adm…