0000000000160554
AUTHOR
Benoit Valot
Plasma membrane in arbuscular mycorrhiza
Protein profiling analyses in arbuscular mycorrhizal symbiosis
Cellular and subcellular studies of the arbuscular mycorrhizal symbiosis in M. truncatula: a proteomic survey
A Medicago truncatula root plasma membrane enriched fraction as a prerequisite for label free quantitative analysis of plasmalemma protein changes upon arbuscular mycorrhizal symbiosis
High Prevalence of Human-Associated Escherichia coli in Wetlands Located in Eastern France
International audience; Escherichia coli that are present in the rivers are mostly brought by human and animal feces. Contamination occurs mostly through wastewater treatment plant (WWTP) outflows and field amendment with sewage sludge or manure. However, the survival of these isolates in river-associated wetlands remains unknown. Here, we assessed E. coli population structure in low-anthropized wetlands located along three floodplains to identify the major source of contamination of wetlands, whose functioning is different from the rivers. We retrieved 179 E. coli in water samples collected monthly from 19 sites located in eastern France over 1 year. Phylogroups B1 and B2 were dominant in …
Could subcellular proteomics of root plastids teach us more about mycorrhizal symbiosis?
International audience; The arbuscular mycorrhizal (AM) symbiosis is a mutualistic association between soil-borne fungi and the roots of most plant species. Involving the bilateral exchange of nutrients, the symbiosis is connected to drastic changes in plant cell organelle morphology and physiology. Root plastids, in particular, are forming extensive, network-like structures covering the main symbiotic interface, i.e., intracellular, highly branched haustorium-like fungal structures called arbuscules. These plastid networks are highly dynamic and are formed and degraded concomitantly with the formation and degradation of arbuscules. By producing basic metabolic building blocks like fatty ac…
Studying the arbuscular mycorrhizal symbiosis from axenic system to soilborne interactions by proteomics: which challenges to face?
Membrane proteomic of arbuscular mycorrhiza
Membrane proteomic analyses to reveal and identify arbuscular mycorrhizal-related proteins
Proteomics: a functional genomic approach for studying mycorrhizal interactions in legumes
Subcellular proteomics sheds light on root plastid involvement in Medicago truncatula arbuscular mycorrhizal symbiosis
Technical improvements for analysis of récalcitrant proteins by LC-MS
Membrane proteomics of arbuscular mycorrhiza
International audience
Mycorrhization-induced changes in the root plastid proteome of Medicago truncatula
Detection of Temporal Clusters of Healthcare-Associated Infections or Colonizations with Pseudomonas aeruginosa in Two Hospitals: Comparison of SaTScan and WHONET Software Packages.
International audience; The identification of temporal clusters of healthcare-associated colonizations or infections is a challenge in infection control. WHONET software is available to achieve these objectives using laboratory databases of hospitals but it has never been compared with SaTScan regarding its detection performance. This study provided the opportunity to evaluate the performance of WHONET software in comparison with SaTScan software as a reference to detect clusters of Pseudomonas aeruginosa. A retrospective study was conducted in two French university hospitals. Cases of P. aeruginosa colonizations or infections occurring between 1st January 2005 and 30th April 2014 in the fi…
The arbuscular mycorrhizal symbiosis a modulator of cadmium stress
International audience; Ecosystems are submitted to various abiotic stresses, among which heavy metals represent major industrial pollutants. Cadmium (Cd), that has damaging effects on plant metabolism, occurs in agricultural environments through industrial pollution and human activities, including phosphate fertiliser and sewage sludge applications. Metal availability to plants can be modulated by soil microorganisms, such as arbuscular mycorrhizal (AM) fungi. In the present work, Cd effects on the model legume Medicago truncatula inoculated or not with the AM fungus Glomus intraradices have been studied at 3 levels: (1) plant biomass production together with green part chlorophyll quantif…
Proteomes of arbuscular mycorrhizal symbiosis : past and present achievements
Label-free 1-DE-LC-MS/MS and iTRAQ-OFFGEL-LC-MS/MS to identify arbuscular mycorrhiza-related membrane proteins.
Plant mycorrhizal interaction: What can we learn from a proteomic approach?
Populations of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae are different in human-polluted environment and food items: a multicentre European study
Abstract Objectives To assess the extent to which food items are a source of extended-spectrum β-lactamase (ESBL) -producing Escherichia coli (ESBL-Ec) and ESBL-producing Klebsiella pneumoniae (ESBL-Kp) for humans in five European cities. Methods We sampled 122 human polluted (hp)-environments (sewers and polluted rivers, as a proxy of human contamination) and 714 food items in Besancon (France), Geneva (Switzerland), Sevilla (Spain), Tubingen (Germany) and Utrecht (The Netherlands). A total of 254 ESBL-Ec and 39 ESBL-Kp isolates were cultured. All genomes were fully sequenced to compare their sequence types (ST) and core genomes, along with the distribution of blaESBL genes and their genet…
Functional analysis of the membrane proteome of Medicago truncatula roots upon colonization by the arbuscular mycorrhizal fungus Glomus irregulare
International audience
Arbuscular mycorhizal proteomes: what news at the nearby and distant horizon?
International audience; Proteomics has soon emerged as a powerful tool to point out protein modifications in roots interacting with arbuscular mycorrhiza (AM) fungi. Depending on the developmental mycorrhizal stage and on the available amount of mycorrhizal material, untargeted and/or sub-cellular proteomic approaches were applied to reveal and identify proteins whose accumulation was modified during the AM colonisation of Medicago truncatula roots. For the early stage of symbiosis, the protein patterns obtained from noninoculated roots and roots synchronized for appressorium formation in wild-type (Jemalong J5), penetration-defective (TRV25, dmi3) and autoregulation-defective (TR122, sunn)…
Contribution of proteomics to arbuscular mycorrhiza in Medicago truncatula
International audience; Because proteins are key effectors of plant responses to environmental cues including recognition, signalling, transport and defence reactions, main interest has been paid to characterize those involved in the establishment and functioning of arbuscular mycorrhizal (AM) symbiosis. In our group, the setting up of high throughput proteomic techniques on the model species, Medicago truncatula, is providing step-by-step a large-scale analysis of AM symbiosis-related proteins. Depending on the symbiotic stage targeted and on the abundance of mycorrhizal material, different proteomic strategies that can be combined with other large-scale approaches (transcriptomic and meta…