0000000000162599
AUTHOR
Arnaud Hélary
IMAGINE: A Cold Neutron Imaging Station at the Laboratoire Léon Brillouin
International audience; A second cold neutron imaging station has been open to users at the Laboratoire Léon Brillouin. The station is designed for high resolution neutron imaging and tomography. The typical field of view is 100x100 mm2 with a spatial resolution of 100 μm. Betterspatial resolutions (∼50 μm) can be achieved when reducing the field of view down to 30x30mm2. The L/D ratio can be varied from 200 to1000with pinhole sizes ranging from 20 to7 mm. Future upgrades will provide capabilities for energy resolved measurements using either a velocity selector or a double crystal monochromator. The possibility to perform polarized neutron experiments will also be provided next year.
Effect of Ligands on HP-Induced Unfolding and Oligomerization of β-Lactoglobulin
ABSTRACTTo probe intermediate states during unfolding and oligomerization of proteins remains a major challenge. High pressure (HP) is a powerful tool for studying these problems, revealing subtle structural changes in proteins not accessible by other means of denaturation. Bovine β-lactoglobulin (BLG), the main whey protein, has a strong propensity to bind various bioactive molecules, such as retinol and resveratrol, two ligands with different affinity and binding sites. By combining in situ HP-small-angle neutron scattering (SANS) and HP-UV/visible absorption spectroscopy, we report the specific effects of these ligands on 3D conformational and local changes in BLG induced by HP. Dependin…
Neutron imaging and tomography: Applications in food science
International audience; Neutron imaging and tomography allow observing the structure of large objects (from few to hundred centimeters) at a resolution around 100 μm. This non-destructive method is based on the detection of the transmission of a neutron beam through an object in 2D (imaging) or 3D after rotating the sample (tomography). The components of the object attenuate the neutron beam differently, depending on their composition, and produce accurate pictures of objects which give information about their structure. Neutron imaging has been used for quality control purposes in industries (aircraft, motor engineering...), but also find application from materials sciences (fuel cells, li…