Recombinant human vasostatin-1 in adult rat myocardiocytes: interactions with Heat Shock Protein 90, endothelial nitric oxide synthase expression and extracellular matrix components

3D cultures as an in vitro model to study human vasostatin-1 effects

Cell- extracellular matrix interaction: a putative non-conventional target of vasostatin-1

Human recombinant vasostatin-1 may interfere with cell-extracellular matrix interactions

Vasostatin-1 (VS-1), the N-terminal fragment derived from the cleavage of chromogranin A (CgA), has been shown to exert several biological activities on several tissues and organs. Recently, it has been reported that human recombinant VS-1 (STA-CGA(1-78)) may alter myocardial contractility in eel, frog., and rat hearts. In this article we have explored if STA-CGA(1-78) can induce intracellular cascades interacting both with adhesion molecules and/or extracellular matrix (ECM), components, that is, involvement of the heat shock protein 90 (HSP90) and the endothelial NOS (eNOS), known to be implicated in signal transduction mechanisms affecting myocardial contractility. We used 3D cultured ad…

Cell-extracellular matrix interaction: a putative non-conventional target of Vasostatin-1. A confocal analysis