0000000000190676
AUTHOR
L. Valero
iTRAQ and DIGE in the ecophysiological characterization of zebra mussel populations (Dreissena polymorpha) invading the Ebro and the Júcar hydrographic basins
Poster presentado en el 9th Iberian and 6th Iberoamerican Congress on Environmental Contamination and Toxicology "The environmental research: essential for sustainability" celebrado en Valencia del 1 al 3 de julio de 2013
An insight into the proteome of the saliva of the argasid tick Ornithodoros moubata reveals important differences in saliva protein composition between the sexes
26 páginas, 3 tablas, 6 figuras. -- The definitive version is available at http://www.elsevier.com
Evolutionary Changes after Translational Challenges Imposed by Horizontal Gene Transfer
International audience; Genes acquired by horizontal gene transfer (HGT) may provide the recipient organism with potentially new functions, but proper expression level and integration of the transferred genes in the novel environment are not granted. Notably, transferred genes can differ from the receiving genome in codon usage preferences, leading to impaired translation and reduced functionality. Here, we characterize the genomic and proteomic changes undergone during experimental evolution of Escherichia coli after HGT of three synonymous versions, presenting very different codon usage preference, of an antibiotic resistance gene. The experimental evolution was conducted with and without…
Proteomic analysis of the somatic and surface compartments from Dirofilaria immitis adult worms.
31 páginas, 1 tabla, 4 figuras.-- The definitive version is available at http://www.elsevier.com
Identification of antigenic proteins from Echinostoma caproni (Trematoda) recognized by mouse immunoglobulins M, A and G using an immunoproteomic approach.
Antigenic proteins of Echinostoma caproni (Trematoda) against mouse IgM, IgA, IgG, IgG1 and IgG2a were investigated by immunoproteomics. Excretory/secretory products (ESP) of E. caproni separated by two-dimensional (2D) gel electrophoresis were transferred to nitrocellulose membranes and probed with the different mouse immunoglobulin classes. A total of four proteins (enolase, 70 kDa heat-shock protein (HSP-70), actin and aldolase) were accurately identified. Enolase was recognized in eight different spots of which seven of them were detected in the expected molecular weight and were recognized by IgA, IgG or IgG and IgG1. Another spot identified as enolase at 72 kDa was only recognized by …