0000000000206599
AUTHOR
Johannes Reusch
Resolving Binding Events on the Multifunctional Human Serum Albumin
Abstract Physiological processes rely on initial recognition events between cellular components and other molecules or modalities. Biomolecules can have multiple sites or mode of interaction with other molecular entities, so that a resolution of the individual binding events in terms of spatial localization as well as association and dissociation kinetics is required for a meaningful description. Here we describe a trichromatic fluorescent binding‐ and displacement assay for simultaneous monitoring of three individual binding sites in the important transporter and binding protein human serum albumin. Independent investigations of binding events by X‐ray crystallography and time‐resolved dyn…
CCDC 1917091: Experimental Crystal Structure Determination
Related Article: Lea Wenskowsky, Michael Wagner, Johannes Reusch, Herman Schreuder, Hans Matter, Till Opatz, Stefan Matthias Petry|2020|ChemMedChem|15|738|doi:10.1002/cmdc.202000069